ICAM-1/CD54 Antibody (ST0487)

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human kidney tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human lung tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human spleen tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: ST0487
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ICAM-1/CD54 Antibody (ST0487) Summary

• Description: Novus Biologicals Rabbit ICAM-1/CD54 Antibody (ST0487) (NBP2-67518) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human ICAM-1/CD54 aa 30-70. (SwissProt: P05362 Human)
• Localization: Membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ICAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (ST0487)

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM1