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ICAM-1/CD54 Antibody (1A29)

Images

 
Biological Strategies: Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of ...read more
Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.
Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).
Characteristic expression of MTH1 in human gastric cancer tissues and ten digestive tract cancer cell lines.a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level ...read more
Comparison of Ki-67 or CD11b immunoreactivity of Aldara-treated dorsal skin samples of C57BL/6 mice using OP or MP. Nuclear proliferation marker Ki-67 immunostaining on OP (a) and MP (b) dorsal skin tissue samples at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, MS, B/N, IHC
Clone
1A29
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

ICAM-1/CD54 Antibody (1A29) Summary

Immunogen
Rat lymph node stroma
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Electron Microscopy
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Proximity Ligation Assay
  • Western Blot 1 - 3 ug/ml
Application Notes
Use in PLA reported in scientific literature (PMID:34572594). Use in Immunohistochemistry free floating, and Electron Microscopy reported in scientific literature (PMID: 8574890). .
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-22541 in the following applications:

Publications
Read Publications using
NBP2-22541 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for ICAM-1/CD54 Antibody (1A29)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (NBP2-22541)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: B/N, EM, FLOW, IF/IHC, IHC-FrFl, IHC-P, PLA, Simple Western, WB.


Filter By Application
B/N
(2)
EM
(1)
FLOW
(3)
IF/IHC
(1)
IHC-FrFl
(1)
IHC-P
(2)
PLA
(1)
Simple Western
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(4)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP2-22541 Applications Species
Xu Z, Wang X, Kuang W et al. Kaempferol improves acute kidney injury via inhibition of macrophage infiltration in septic mice Bioscience Reports 2023-07-26 [PMID: 37440431] (WB, B/N) WB, B/N
Liu Q, Li C, Deng B et al. Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development Cell reports 2023-02-28 [PMID: 36857185] (FLOW, IHC-P, Mouse) FLOW, IHC-P Mouse
Sulimai N, Brown J, Lominadze D The Effects of Fibrinogen's Interactions with Its Neuronal Receptors, Intercellular Adhesion Molecule-1 and Cellular Prion Protein Biomolecules 2021-09-18 [PMID: 34572594] (PLA, Mouse) PLA Mouse
Sulimai N, Brown J, Lominadze D. Fibrinogen Interaction with Astrocyte ICAM-1 and PrP(C) Results in the Generation of ROS and Neuronal Death International Journal of Molecular Sciences 2021-02-27 [PMID: 33673626] (B/N) B/N
Vazquez Rodriguez G, Abrahamsson A, Turkina MV, Dabrosin C. Lysine in Combination With Estradiol Promote Dissemination of Estrogen Receptor Positive Breast Cancer via Upregulation of U2AF1 and RPN2 Proteins Frontiers in Oncology 2020-11-30 [PMID: 33330095] (WB) WB
Sadhukhan R, Leung J. W. C, et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 2020-05-07 [PMID: 32382091] (IF/IHC, Mouse) IF/IHC Mouse
Hlavac N, VandeVord PJ. Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins Front Neurol 2019-02-22 [PMID: 30853931] (Simple Western, Rat) Simple Western Rat
Vazquez Rodriguez G, Abrahamsson A, Jensen LDE et al. Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8 Front. Immunol 2018-07-30 [PMID: 30105032] (WB, Human)

Details:
This citation used the Azide and BSA Free version of this antibody.
WB Human
Pistorius K, Souza PR, De Matteis R et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 [PMID: 29805036] (Flow) Cell Chem Biol. 2018-05-24 [PMID: 29805036] (FLOW)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
FLOW
Hu S, Liu Y, You T et al. Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial B1 Integrin. Arterioscler. Thromb. Vasc. Biol. 2017-12-21 [PMID: 29269512] (Mouse) Mouse
Show All 17 Publications.

Review for ICAM-1/CD54 Antibody (NBP2-22541) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse and Mouse lung.

Reviews using NBP2-22541:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse and Mouse lung
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ICAM-1/CD54 NBP2-22541
Enlarge
4
reviewed by:
James Xiao
IHC-Fr Mouse and Mouse lung 03/20/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLung tissue
SpeciesMouse and Mouse lung
LotRE228542

Comments

CommentsFrozen section of the mouse lung was stained with 1:100 dilution of ICAM-1 ab (NBP-2-22541) for 1 hr RT. Tissue was washed and stained with Alexa-594 Goat anti-mouse antibody for an additional of 30 minutes RT. Section was then washed and analyzed by fluorescence microbe.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ICAM-1/CD54 Antibody (NBP2-22541). (Showing 1 - 1 of 1 FAQs).

  1. Our customer found it difficult to choose between NBP2-22541 and NB100-65608. They will perform IHC-P with muscle sections from rat. Would you please help recommend one?
    • Both NBP2-22541 and NB100-65608 are covered by our 100% guarantee for IHC-P with rat tissue. Please find more information about our quality guarantee, which you may wish to pass on to your customer. Your customer may be interested in our antibody with catalogue number NB500-318. This has also been validated for IHC-P with rat tissue, and furthermore has a publication citing its use for IHC-P with rat heart tissue. Here is a link to the product page of this antibody NB500-318.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
0
4
1
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2
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1
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James Xiao
03/20/2017
Application: IHC-Fr
Species: Mouse and Mouse lung

Bioinformatics

Gene Symbol ICAM1
Entrez