ICAM-1/CD54 Antibody (009) - Azide and BSA Free

Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Immunofluorescence staining of Mouse ICAM-1/CD54 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90585 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 009
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ICAM-1/CD54 Antibody (009) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ICAM-1/CD54 (Accession#: NP_034623.1; Met1-Asn485).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ICAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (009) - Azide and BSA Free

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (NBP2-90585)(1)

Publication using NBP2-90585

• Chen M, Kim S, Li L et al. Identification of an adipose tissue-resident pro-preadipocyte population Cell reports 2023-04-27 [PMID: 37119138] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ICAM1