Home » HUS1B » HUS1B Antibodies » HUS1B Antibody

HUS1B Antibody

 Submit a Review

Images

 
(
Enlarge)
Immunocytochemistry/Immunofluorescence: HUS1B Antibody [NBP2-56691] - Staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunocytochemistry/ Immunofluorescence: HUS1B Antibody [NBP2-56691] - Staining of human cell line HEK 293 shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

HUS1B Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GASSLKLQLTHKRRPSLTVAVELVSSLGRARSVVHDLPVRVLPRRVWRDCLPPSLRASDASIRLPRWRTLRSIVERMANVGSHVL
Specificity
Specificity of human HUS1B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HUS1B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HUS1B Recombinant Protein Antigen (NBP2-56691PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for HUS1B Antibody

  • checkpoint protein HUS1B
  • hHUS1B
  • HUS1 (S. pombe) checkpoint homolog b
  • HUS1 checkpoint homolog b (S. pombe)
  • MGC126746
  • MGC126748
  • RP11-532F6.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HUS1 Antibody
NBP1-33589
Western Blot: HUS1 Antibody [NBP1-33589] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-33589] - HUS1 antibody detects HUS1 protein at cytosol and nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. HUS1 antibody dilution: 1:500.
Western Blot: HUS1 Antibody [NBP1-33589] - 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Rad1 Antibody (1G2)
H00005810-M01J
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Western blot analysis of Rad1 over-expressed 293 cell line, cotransfected with Rad1 Validated Chimera RNAi [H00005810-R01V] (Lane 2) or non-transfected control (Lane 1). Blot probed with Rad1 monoclonal antibody (M01), clone 1G2 [H00005810-M01]. GAPDH ( 3
Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J] - Immunoperoxidase of monoclonal antibody to Rad1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01J), clone 1G2. Lane 1: RAD1 transfected lysate(32.43 KDa).Lane 2: Non-transfected lysate.
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, RNAi, S-ELISA, ELISA(Cap)
XPF Antibody
(OTI4E11) - Azide and ...
NBP2-74883
Western Blot: XPF Antibody (OTI4E11) - Azide and BSA Free [NBP2-74883] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.
Immunocytochemistry/Immunofluorescence: XPF Antibody (OTI4E11) - Azide and BSA Free [NBP2-74883] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.
Immunohistochemistry: XPF Antibody (OTI4E11) - Azide and BSA Free [NBP2-74883] - Staining of paraffin-embedded Human prostate tissue using anti-XPF mouse monoclonal antibody.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
Rad9 Antibody
(93A535)
NB120-13600
Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.
Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
(8 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
RRAD Antibody
NBP2-13266
Immunocytochemistry/Immunofluorescence: RRAD Antibody [NBP2-13266] - Staining of human cell line A549 shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
Immunohistochemistry-Paraffin: RRAD Antibody [NBP2-13266] - Staining of human heart muscle shows moderate membranous positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PCNA Antibody
(PC10)
NB500-106
Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
(5 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Rad17 [p Ser645]
Antibody
NBP2-24457
Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml.
Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - Western blot analysis of phosphorylated Rad17 (Ser645) in recombinant fusion protein containing 1) a phosphorylated serine at position 645 and 2) an unphosphorylated serine at position 645 using NBP2-24457 at 0.025 ug/ml.
Immunohistochemistry-Paraffin: Rad17 [p Ser645] Antibody [NBP2-24457] - Analysis of phosphorylated Rad17 in formalin-fixed, paraffin-embedded mouse liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
Chk1 Antibody
NB100-464
Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/19084), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(8 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
FEN-1 Antibody
(4E7)
NB100-150
Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.
(17 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IP
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
APE Antibody
(13B8E5C2)
NB100-116
Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Knockdown Validated: APE Antibody (13B8E5C2) [NB100-116] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1, p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
(3 Reviews)
(93 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD
ATR Antibody (2B5)
NB100-308
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
(18 Publications)
Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP, In vitro, KO
TEM8/ANTXR1
Antibody (200C1339(SB
NB100-56585
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
(5 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MYH Antibody (4D10)
H00004595-M01
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.
(10 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, RNAi, S-ELISA
RAD9B Antibody
NBP2-85585
Western Blot: RAD9B Antibody [NBP2-85585] - Host: Rabbit. Target Name: RAD9B. Sample Type: Fetal Thymus lysates. Antibody Dilution: 1.0ug/ml
Western Blot: RAD9B Antibody [NBP2-85585] - Host: Rabbit. Target Name: RAD9B. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml
Species: Hu
Applications: WB
MMAB Antibody
(OTI2G5)
NBP2-03417
Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry-Paraffin: MMAB Antibody (2G5) [NBP2-03417] Staining of paraffin-embedded Human lymph node tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Bim Antibody
NBP1-76963
Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
(7 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
PUMA Antibody
NB100-56370
Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.
Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.
(6 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
MSH6 Antibody (3E1)
NBP2-37537
Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: MSH6 Antibody (3E1) [NBP2-37537] - Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of colon cancer tissues using MSH6 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
DNA Ligase I
Antibody (10H5)
NB100-119
Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Detection of human Ligase I protein in Raji whole cell lysate using NB 100-119.
(23 Publications)
Species: Hu, Mu, Ba, Ch, Fi, Ha, Rb
Applications: WB, ICC/IF, IP, In vitro, RIA

Publications for HUS1B Antibody (NBP2-56691) (0)

There are no publications for HUS1B Antibody (NBP2-56691).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HUS1B Antibody (NBP2-56691) (0)

There are no reviews for HUS1B Antibody (NBP2-56691). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for HUS1B Antibody (NBP2-56691) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional HUS1B Products

Array NBP2-56691

Bioinformatics Tool for HUS1B Antibody (NBP2-56691)

Discover related pathways, diseases and genes to HUS1B Antibody (NBP2-56691). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HUS1B Antibody (NBP2-56691)

Discover more about diseases related to HUS1B Antibody (NBP2-56691).
 

Pathways for HUS1B Antibody (NBP2-56691)

View related products by pathway.

PTMs for HUS1B Antibody (NBP2-56691)

Learn more about PTMs related to HUS1B Antibody (NBP2-56691).

Blogs on HUS1B

There are no specific blogs for HUS1B, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HUS1B Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HUS1B