DNA Ligase I Antibody (10H5)

Images

 
Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The ...read more
Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at ...read more
Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Ba, Ch, Fi, Ha, RbSpecies Glossary
Applications WB, ICC/IF, IHC-P, In vitro, IP, RIA
Clone
10H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DNA Ligase I Antibody (10H5) Summary

Immunogen
Full-length recombinant human DNA Ligase I protein
Localization
Nuclear
Specificity
This is specific for DNA ligase 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIG1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • In vitro assay
  • Radioimmunoassay
  • Western Blot
Application Notes
ICC/IF, IP usage reported in (PMID: 11912211). Use in Radioimmunoassay reported in (PMID: 9603940). Use In vitro assay reported in scientific literature (PMID: 11912211). WB, ICC/IF, IHC-P, IP-Assay dependent.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-119 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.. Rabbit reactivity reported in scientific literature (PMID: 12668657). Hamster reactivity reported in scientific literature (PMID: 11912211). Fish, and chicken reactivity reported in scientific literature (PMID: 12928478). Bacteria reactivity reported in scientific literature (PMID: 9603940).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for DNA Ligase I Antibody (10H5)

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repairand recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Ba, Ch, Fi, Ha, Rb
Applications: WB, ICC/IF, IHC-P, In vitro, IP, RIA

Publications for DNA Ligase I Antibody (NB100-119)(23)

We have publications tested in 7 confirmed species: Human, Bacteria, Human, Chicken, Fish, Hamster, Rabbit.

We have publications tested in 4 applications: Bacteria, ICC/IF, IP, WB.


Filter By Application
Bacteria
(1)
ICC/IF
(1)
IP
(4)
WB
(2)
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Filter By Species
Human
(7)
Bacteria
(1)
Human
(5)
Chicken
(1)
Fish
(1)
Hamster
(1)
Rabbit
(1)
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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-119 Applications Species
Jaiswal AS, Narayan S. Assembly of the Base Excision Repair Complex on Abasic DNA and Role of Adenomatous Polyposis Coli on Its Functional Activity. Biochemistry;50(11):1901-9. 2011 Mar 22. [PMID: 21261287]
Asagoshi K, Liu Y, Masaoka A et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst) 2010 Feb [PMID: 20006562] (Human) Human
Guo Z, Zheng L, Dai H et al. Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity. Nucleic Acids Res 2009 Jun [PMID: 19336415] (Human) Human
Song W, Levin DS, Varkey J et al. A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes. J Biol Chem 2007 Aug [PMID: 17561505] (Human) Human
Windhofer F, Wu W, Iliakis G. Low levels of DNA ligases III and IV sufficient for effective NHEJ. J Cell Physiol 2007 Nov [PMID: 17492771] (Human) Human
Rose, J L et al. Base Excision Repair Proteins Are Required for Integrin-Mediated Suppression of Bleomycin-Induced DNA Breakage in Murine Lung Endothelial Cells. J. Pharmacol. Exp. Ther. 321: 318-326. 2007 [PMID: 17202402]
Wang W, Lindsey-Boltz LA, Sancar A et al. Mechanism of stimulation of human DNA ligase I by the Rad9-rad1-Hus1 checkpoint complex. J Biol Chem 2006 Jul [PMID: 16731526] (Human) Human
Wang H, Rosidi B, Perrault R et al. DNA ligase III as a candidate component of backup pathways of nonhomologous end joining. Cancer Res 2005 May [PMID: 15899791] (Human) Human
Warren, M et al. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum. Mol. Genetics. 12:2645-2656. 2003 [PMID: 12928478] (Fish, Chicken) Fish, Chicken
Kao, GD et al. Histone deacetylase 4 interacts with 53BPI to mediate the DNA damage response. J. Cell. Biol. 160:1017-1027. 2003 [PMID: 12668657] (Rabbit) Rabbit
Show All 23 Publications.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

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Bioinformatics

Gene Symbol LIG1
Entrez