Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The ...read more
Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at ...read more
Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ICC/IF, IP usage reported in (PMID: 11912211). Use in Radioimmunoassay reported in (PMID: 9603940). Use In vitro assay reported in scientific literature (PMID: 11912211). WB, ICC/IF, IHC-P, IP-Assay dependent.
125 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.. Rabbit reactivity reported in scientific literature (PMID: 12668657). Hamster reactivity reported in scientific literature (PMID: 11912211). Fish, and chicken reactivity reported in scientific literature (PMID: 12928478). Bacteria reactivity reported in scientific literature (PMID: 9603940).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Protein G purified
Alternate Names for DNA Ligase I Antibody (10H5)
DNA ligase 1
DNA ligase I
ligase I, DNA, ATP-dependent
Polydeoxyribonucleotide synthase [ATP] 1
polydeoxyribonucleotide synthase ATP 1
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repairand recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Using PCNA as an Antibody Marker PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ... Read full blog post.
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