MYH Antibody (4D10)

Knockdown Validated: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 hrs of Dox treatment of cells with shRNA set1 (d). Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. The graphs represent mean +/-s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, NS, not significant. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/oncsis201672) licensed under a CC-BY license.

Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.

Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.

ELISA: MYH Antibody (4D10) [H00004595-M01] - Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP, KD
• Clone: 4D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MYH Antibody (4D10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
• Localization: Nuclear
• Specificity: MUTYH - mutY homolog (E. coli)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUTYH
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MYH Antibody (4D10)

• EC 3.2.2
• EC 3.2.2.-
• EC 3.2.2.31
• hMYH
• MGC4416
• MutY homolog
• MUTYH
• Muyth
• MYH
• MYHA/G-specific adenine DNA glycosylase

Background

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH Antibody (H00004595-M01)(10)

Publications using H00004595-M01

• Eshtad S, Mavajian Z, Rudd SG et al. hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia. Oncogenesis 2016-12-05 [PMID: 27918552]
• Shinmura K, Kato H, Kawanishi Y et al. Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma. Mol Carcinog 2016-06-02 [PMID: 27253753]
• Komine K, Shimodaira H, Takao M et al. Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli strain. Hum Mutat. 2015-03-27 [PMID: 25820570]
• Agustina L, Hahm SH, Han SH et al. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC Mol Biol. 2014-08-15 [PMID: 25127721]
• Shinmura K, Goto M, Tao H et al. Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer. Oxid Med Cell Longev. 2014-03-23 [PMID: 24799981]
• Hahm SH, Chung JH, Agustina L et al Human MutY homolog induces apoptosis in EPE-treated HEK293 cells Oncol Lett 2012-12-01 [PMID: 23226797]
• Raetz AG, Xie Y, Kundu S et al. Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells. Carcinogenesis. 2012-09-20 [PMID: 22926731]
• Shinmura K, Goto M, Suzuki M et al. Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer. The Journal of Pathology. 2011-11-01 [PMID: 21826668]
• Suzuki T, Harashima H, Kamiya H. Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. DNA Repair (Amst). 2010-02-28 [PMID: 20197241]
• van der Post RS, Kets CM, Ligtenberg MJ et al. Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis. Virchows Arch. 2009-01-01 [PMID: 19031083]

Bioinformatics

• Gene Symbol: MUTYH
• Entrez:
  • 4595(Human)
• Uniprot:
  • AAH03178(Human)