MYH Antibody (4D10)

Images

 
Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: ...read more
Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal ...read more
ELISA: MYH Antibody (4D10) [H00004595-M01] - Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP, RNAi
Clone
4D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MYH Antibody (4D10) Summary

Immunogen
MUTYH (AAH03178 436 a.a. - 535 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
Localization
Nuclear
Specificity
MUTYH - mutY homolog (E. coli)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUTYH
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunoprecipitation
  • RNA Inhibition
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Publications
Read Publications using H00004595-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MYH Antibody (4D10)

  • CYP2C
  • EC 3.2.2
  • EC 3.2.2.-
  • hMYH
  • MGC4416
  • mutY (E. coli) homolog
  • mutY homolog (E. coli)
  • MutY homolog
  • MYHA/G-specific adenine DNA glycosylase

Background

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MYH Antibody (H00004595-M01)(10)


Showing Publications 1 - 10 of 10.
Publications using H00004595-M01 Applications Species
Eshtad S, Mavajian Z, Rudd SG et al. hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia. Oncogenesis 2016 Dec 5 [PMID: 27918552]
Shinmura K, Kato H, Kawanishi Y et al. Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma. Mol Carcinog 2016 Jun 2 [PMID: 27253753]
Komine K, Shimodaira H, Takao M et al. Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli strain. Hum Mutat. 2015 Mar 27 [PMID: 25820570]
Agustina L, Hahm SH, Han SH et al. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC Mol Biol. 2014 Aug 15 [PMID: 25127721]
Shinmura K, Goto M, Tao H et al. Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer. Oxid Med Cell Longev. 2014 Mar 23 [PMID: 24799981]
Hahm SH, Chung JH, Agustina L et al. Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells. Oncol Lett. 2012 Dec 01 [PMID: 23226797]
Raetz AG, Xie Y, Kundu S et al. Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells. Carcinogenesis. 2012 Sep 20 [PMID: 22926731]
Shinmura K, Goto M, Suzuki M et al. Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer. The Journal of Pathology. 2011 Nov [PMID: 21826668]
Suzuki T, Harashima H, Kamiya H. Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. DNA Repair (Amst). 2010 Feb 28 [PMID: 20197241]
van der Post RS, Kets CM, Ligtenberg MJ et al. Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis. Virchows Arch. 2009 Jan 01 [PMID: 19031083]

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Product General Protocols

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Bioinformatics

Gene Symbol MUTYH
Entrez
Uniprot