![Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0006.jpg "Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Po, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Huntingtin. The exact sequence is proprietary. |
| Predicted Species | Porcine (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HTT |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7) |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![ELISA: FMN1 Antibody (4F4) [H00342184-M07] - Detection limit for recombinant GST tagged FMN1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-ELISA-H00342184-M07-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of monoclonal antibody to FMN1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-Immunocytochemistry-Immunofluorescence-H00342184-M07-img0001.jpg)
![Western Blot: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of FMN1 expression in human colon.](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-Western-Blot-H00342184-M07-img0004.jpg)
![Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Flow-Cytometry-NB100-56708-img0043.jpg)
![Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Flow-Cytometry-NB100-56708-img0045.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0044.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Huntingtin Antibody (NBP3-13106)Discover more about diseases related to Huntingtin Antibody (NBP3-13106).
| Pathways for Huntingtin Antibody (NBP3-13106)View related products by pathway.
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Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HTT |
![Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Huntingtin protein stained by Huntingtin antibody (NBP3-13106) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13106-img0003.jpg "Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Huntingtin protein stained by Huntingtin antibody (NBP3-13106) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg "Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg "Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0004.jpg "Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0005.jpg "Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Analysis of CRH expression in transfected 293T cell line by CRH monoclonal antibody (M02), clone 2B11.Lane 1: CRH transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/Corticotropin-Releasing-Factor-Antibody-(2B11)-Western-Blot-H00001392-M02-img0002.jpg)
![Sandwich ELISA: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Detection limit for recombinant GST tagged CRH is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/images2/Corticotropin-Releasing-Factor-Antibody-(2B11)-Sandwich-ELISA-H00001392-M02-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")