Huntingtin Antibody - Azide and BSA Free

Western Blot: Huntingtin Antibody [NBP1-44266] - Western blot analysis on mouse brain extract using 0.1ug/mL of NBP1-44266 (lane 1) and 0.1ug/mL of NBP1-44266 combined with 1ug/mL of blocking peptide (lane 2).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Huntingtin Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Huntingtin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Huntingtin disease protein. (Uniprot: P42858)
• Localization: Brain
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Western Blot 0.1ug/ml
• Application Notes: This product is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for Huntingtin Antibody - Azide and BSA Free

• HD protein
• HD
• HTT
• huntingtin (Huntington disease)
• Huntingtin
• IT15
• IT15HDHuntington disease protein

Background

Huntingtonʹs disease (HD) is an autosomal dominant neurological disorder caused by a polyglutamine (polyQ) repeat expansion in the huntingtin (Htt) protein. The disease is characterized by neurodegeneration and formation of neuronal intracellular inclusions primarily in the striatum and cortex, leading to personality changes, motor impairment, and dementia. The Huntington’s disease protein is ~350kD in size and is localized in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Huntingtin Antibody (NBP1-44266)(3)

Publications using NBP1-44266

• Imarisio S, Carmichael J, Korolchuk V et al. Huntington's disease: from pathology and genetics to potential therapies. Biochem J 2008-06-01 [PMID: 18466116]
• Vonsattel JP. Huntington disease models and human neuropathology: similarities and differences. Acta Neuropathol. 2008-01-01 [PMID: 17978822]
• CHD proteins: a diverse family with strong ties Hall JA, Georgel PT. CHD proteins: a diverse family with strong ties. Biochem Cell Biol;85(4):463-76. 2007-08-01 [PMID: 17713581]

Bioinformatics

• Gene Symbol: HTT
• Entrez:
  • 3064(Human)
• Uniprot:
  • AAB38240(Human)
  • P42858(Human)