Human Clusterin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Clusterin Quantikine ELISA Kit Summary

• Background: The Quantikine Human Clusterin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Clusterin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Clusterin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Clusterin showed linear curves that were parallel to the stand...ard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Clusterin.
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• Specificity: Natural and recombinant human Clusterin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CLU

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Clusterin Quantikine ELISA Kit

• 40
• 40, sulfated glycoprotein 2
• Aging-associated gene 4 protein
• aging-associated protein 4
• APOJ
• apo-J
• Apolipoprotein J
• CLI
• CLIclusterin (complement lysis inhibitor, SP-40
• CLU
• Clusterin
• Complement cytolysis inhibitor
• complement lysis inhibitor
• Complement-associated protein SP-40
• Ku70-binding protein 1
• KUB1SGP2
• MGC24903
• NA1/NA2
• SGP-2
• SP-40
• sulfated glycoprotein 2
• Testosterone-repressed prostate message 2
• testosterone-repressed prostate message 2, apolipoprotein J)
• TRPM-2
• TRPM-2TRPM2

Background

Clusterin [also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40], is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, brain, and in many epithelial tissues during embryonic development (3). Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain (4 - 6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an approximately 80 kDa N-glycosylated and disulfide-linked heterodimer (7-9). Mature human Clusterin shares a 77% amino acid sequence identity with mouse and rat Clusterin. 

High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (10, 11). The ability of Clusterin to bind and neutralize non-oxidatively modified LDL reduces cytotoxicity in atherosclerotic plaques (12). The chaperone function of Clusterin helps to reduce the accumulation of beta -amyloid fibrils and damage due to amyloid plaques in Alzheimer's disease (13). An alternately spliced 50 kDa isoform of human Clusterin (nCLU) remains intracellular and is neither glycosylated nor cleaved into alpha and beta chains (5, 14). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 14). This function contrasts with the cytoprotective effect of secreted Clusterin (15). During tumor progression, nCLU is down regulated while the secreted form is upregulated and may be aberrantly glycosylated (14, 16, 17). Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting the epithelial to mesenchymal transition (18-20).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Clusterin/APOJ (DCLU00)(11)

Publications using DCLU00

• D Holmannova, P Borsky, L Borska, C Andrys, K Hamakova, V Rehacek, T Svadlakova, A Malkova, M Beranek, V Palicka, J Krejsek, Z Fiala Metabolic Syndrome, Clusterin and Elafin in Patients with Psoriasis Vulgaris Int J Mol Sci, 2020;21(16):. 2020 [PMID: 32764517] (Human)
• V Yu, D Bhattachar, A Webster, A Bauskar, C Flowers, M Heur, SK Chintala, T Itakura, MR Wilson, JT Barr, S Jeong, M Wang, ME Fini Clusterin from human clinical tear samples: Positive correlation between tear concentration and Schirmer strip test results Ocul Surf, 2018;0(0):. 2018 [PMID: 30077709] (Human)
• L Donner, L Gremer, T Ziehm, CGW Gertzen, H Gohlke, D Willbold, M Elvers Relevance of N-terminal residues for amyloid-? binding to platelet integrin ?IIb?3, integrin outside-in signaling and amyloid-? fibril formation Cell. Signal., 2018;0(0):. 2018 [PMID: 29964150] (Human)
• GS Choi, HKT Trinh, EM Yang, YM Ye, YS Shin, SH Kim, HS Park Role of clusterin/progranulin in toluene diisocyanate-induced occupational asthma Exp. Mol. Med., 2018;50(5):58. 2018 [PMID: 29717106] (Human)
• KR Ting, M Henry, J Meiller, A Larkin, M Clynes, P Meleady, D Bazou, P Dowling, P O'Gorman Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients BBA Clin, 2017;8(0):28-34. 2017 [PMID: 28725572] (Human)
• JL Hsu, WJ Lee, YC Liao, JF Lirng, SJ Wang, JL Fuh Plasma biomarkers are associated with agitation and regional brain atrophy in Alzheimer's disease Sci Rep, 2017;7(1):5035. 2017 [PMID: 28698646] (Human)
• JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28132833] (Human)
• Mayado A, Teodosio C, Garcia-Montero A, Matito A, Rodriguez-Caballero A, Morgado J, Muniz C, Jara-Acevedo M, Alvarez-Twose I, Sanchez-Munoz L, Matarraz S, Caldas C, Munoz-Gonzalez J, Escribano L, Orfao A Increased IL6 plasma levels in indolent systemic mastocytosis patients are associated with high risk of disease progression. Leukemia, 2016;30(1):124-30. 2016 [PMID: 26153655] (Human)
• Zhou Y, Hayashi I, Wong J, Tugusheva K, Renger J, Zerbinatti C Intracellular clusterin interacts with brain isoforms of the bridging integrator 1 and with the microtubule-associated protein Tau in Alzheimer's disease. PLoS ONE, 2014;9(7):e103187. 2014 [PMID: 25051234] (Human)
• Doudevski , Ivo, Rostagno , Agueda, Cowman , Mary, Liebmann , Jeffrey, Ritch , Robert, Ghiso , Jorge Clusterin and complement activation in exfoliation glaucoma. Invest Ophthalmol Vis Sci, 2014;55(4):2491-9. 2014 [PMID: 24550356] (Human)
• Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S Semen clusterin is a novel DC-SIGN ligand. J. Immunol., 2011;187(10):5299-309. 2011 [PMID: 22013110] (Human)

Bioinformatics

• Gene Symbol: CLU