The Quantikine Human Clusterin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Clusterin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Clusterin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Clusterin showed linear curves that were parallel to the stand...ard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Clusterin.
Clusterin [also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40],
is a secreted multifunctional protein that was named for its ability to induce cellular clustering.
It binds a wide range of molecules and may function as a chaperone of misfolded extracellular
proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis
(1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart,
brain, and in many epithelial tissues during embryonic development (3). Human Clusterin is
synthesized as a precursor that contains two coiled coil domains, three nuclear localization
signals (NLS), and one heparin binding domain (4 - 6). Intracellular cleavages of the precursor
remove the signal peptide and generate comparably sized alpha and beta chains which are secreted
as an approximately 80 kDa N-glycosylated and disulfide-linked heterodimer (7-9). Mature
human Clusterin shares a 77% amino acid sequence identity with mouse and rat Clusterin.
High μg/mL concentrations of Clusterin circulate predominantly as a component of high
density lipoprotein particles, and these are internalized and degraded through interactions
with LRP-2/Megalin (10, 11). The ability of Clusterin to bind and neutralize non-oxidatively
modified LDL reduces cytotoxicity in atherosclerotic plaques (12). The chaperone function of
Clusterin helps to reduce the accumulation of beta -amyloid fibrils and damage due to amyloid
plaques in Alzheimer's disease (13). An alternately spliced 50 kDa isoform of human Clusterin
(nCLU) remains intracellular and is neither glycosylated nor cleaved into alpha and beta chains (5, 14).
Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus
where it interacts with Ku70 and promotes apoptosis (5, 14). This function contrasts with the
cytoprotective effect of secreted Clusterin (15). During tumor progression, nCLU is down
regulated while the secreted form is upregulated and may be aberrantly glycosylated (14, 16,
17). Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting
apoptosis and by promoting the epithelial to mesenchymal transition (18-20).
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Publications for Clusterin/APOJ (DCLU00)(10)
We have publications tested in 1 confirmed species: Human.
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Diseases for Clusterin/APOJ (DCLU00)
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