Human/Canine/Porcine Insulin Quantikine ELISA Kit

• Reactivity: Hu, Po, Ca
• Applications: ELISA
• Conjugate: HRP

Human/Canine/Porcine Insulin Quantikine ELISA Kit Summary

• Background: The Quantikine Human/Canine/Porcine Insulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Insulin in cell culture supernates, serum, and plasma. It contains S. cerevisiae-expressed recombinant human Insulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Insulin showed linear curves that were parallel to the standa...rd curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Insulin.
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• Specificity: Recombinant human Insulin and natural human, canine, and porcine Insulin. This assay cross-reacts 0.25% with Human Proinsulin.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: INS

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Canine/Porcine Insulin Quantikine ELISA Kit

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Insulin (DINS00)(19)

Publications using DINS00

• Solano-Aguilar, G;Matuszek, G;Matthan, NR;Lichtenstein, AH;Wang, X;Lakshman, S;Barger, K;Urban, JF;Molokin, A;Bennett, RE;Hyman, BT;Lamon-Fava, S; Differential regulation of brain microvessel transcriptome and brain metabolome by western and heart-healthy dietary patterns in Ossabaw pigs Scientific reports 2024-11-28 [PMID: 39609531] (Porcine)
• Al-Dwairi, A;Al-Shboul, O;Al-U'datt, DGF;Saadeh, R;AlQudah, M;Khassawneh, A;Alfaqih, M;Albtoush, A;Hweidi, A;Alnemer, A; Effect of poor glycemic control on the prevalence and determinants of anemia and chronic kidney disease among type 2 diabetes mellitus patients in Jordan: An observational cross-sectional study PloS one 2024-11-14 [PMID: 39541418] (Human)
• Ribeiro, RV;Senior, AM;Simpson, SJ;Tan, J;Raubenheimer, D;Le Couteur, D;Macia, L;Holmes, A;Eberhard, J;O'Sullivan, J;Koay, YC;Kanjrawi, A;Yang, J;Kim, T;Gosby, A; Rapid benefits in older age from transition to whole food diet regardless of protein source or fat to carbohydrate ratio: Arandomised control trial Aging cell 2024-07-16 [PMID: 39011855] (Human)
• Kang, DW;Field, CJ;Patel, D;Fairey, AS;Boulé, NG;Dieli-Conwright, CM;Courneya, KS; Effects of high-intensity interval training on cardiometabolic biomarkers in patients with prostate cancer undergoing active surveillance: a randomized controlled trial Prostate cancer and prostatic diseases 2024-07-15 [PMID: 39009705] (Human)
• Hernandez, JD;Li, T;Ghannam, H;Rau, CM;Masuda, MY;Madura, JA;Jacobsen, EA;Filippis, E; Linking adipose tissue eosinophils, interleukin-4, and leptin in human obesity and insulin resistance JCI insight 2024-01-11 [PMID: 38206766] (Human)
• Buga, A;Harper, DG;Sapper, TN;Hyde, PN;Fell, B;Dickerson, R;Stoner, JT;Kackley, ML;Crabtree, CD;Decker, DD;Robinson, BT;Krystal, G;Binzel, K;Lustberg, MB;Volek, JS; Feasibility and metabolic outcomes of a well-formulated ketogenic diet as an adjuvant therapeutic intervention for women with stage IV metastatic breast cancer: The Keto-CARE trial PloS one 2024-01-02 [PMID: 38166036] (Human)
• Vrachnis, D;Antonakopoulos, N;Fotiou, A;Pergialiotis, V;Loukas, N;Valsamakis, G;Iavazzo, C;Stavros, S;Maroudias, G;Panagopoulos, P;Vlahos, N;Peppa, M;Stefos, T;Mastorakos, G; Is There a Correlation between Apelin and Insulin Concentrations in Early Second Trimester Amniotic Fluid with Fetal Growth Disorders? Journal of clinical medicine 2023-04-28 [PMID: 37176607] (Human)
• B Rhee, RM Mahbubur, C Jin, JS Choi, HW Lim, W Huh, JS Park, J Han, S Kim, Y Lee, J Park Evaluation of safety and anti-obesity effects of DWP16001 in naturally obese dogs Bmc Veterinary Research, 2022-06-22;18(1):237. 2022-06-22 [PMID: 35733159] (Canine)
• N Vrachnis, S Argyridis, D Vrachnis, N Antonakopo, G Valsamakis, C Iavazzo, D Zygouris, N Salakos, A Rodolakis, N Vlahos, G Mastorakos, P Drakakis, Z Iliodromit Increased Fibroblast Growth Factor 21 (FGF21) Concentration in Early Second Trimester Amniotic Fluid and Its Association with Fetal Growth Metabolites, 2021-08-28;11(9):. 2021-08-28 [PMID: 34564397] (Human)
• C Liu, L Zhang, W Zheng, X Liang, L Zhang, Z Tian, G Li Lifestyle Intervention for Overweight/Obese Pregnant Women with Polycystic Ovarian Syndrome: Lessons and Challenges Obesity facts, 2021-07-26;14(4):405-414. 2021-07-26 [PMID: 34311460] (Human)
• BA Gordon, CJ Taylor, JE Church, SD Cousins A Comparison of the Gluco-Regulatory Responses to High-Intensity Interval Exercise and Resistance Exercise International Journal of Environmental Research and Public Health, 2021-01-02;18(1):. 2021-01-02 [PMID: 33401694] (Human)
• K Tokuda, T Ikemoto, Y Saito, K Miyazaki, S Yamashita, S Yamada, S Imura, Y Morine, M Shimada The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells Cell Transplant, 2020-01-01;29(0):9636897209547. 2020-01-01 [PMID: 32878465] (Human)
• PG Saracino, HE Saylor, BR Hanna, RC Hickner, JS Kim, MJ Ormsbee Effects of Pre-Sleep Whey vs. Plant-Based Protein Consumption on Muscle Recovery Following Damaging Morning Exercise Nutrients, 2020-07-10;12(7):. 2020-07-10 [PMID: 32664290] (Human)
• MVC Rudge, FP Souza, JF Abbade, RLS Hallur, JPC Marcondes, F Piculo, G Marini, G Vesentini, L Thabane, SS Witkin, IMP Calderon, AMP Barbosa, MV Rudge, AMP Barbosa, IMP Calderon, FP Souza, JF Abbade, LSR Hallur, F Piculo, G Marini, G Vesentini, L Thabane, MS Palma, CFO Graeff, RK Arni, RD Herculano, DF Salvadori, S Mateus, M Dal Pai Si, CG Magalhães, RA Costa, SAM Lima, SL Felisbino, W Barbosa, A Atallah, MJB Girão, Z Di Bella, SM Uchoa, S Payão, A Hijas, B Berghman, R De Bie, L Sobrevia, B Junginger, FCB Alves, PS Rossignoli, CB Prudencio, MIG Orlandi, MI Gonçalves, SK Nunes, BB Catinelli, S Quiroz, BV Sarmento, FA Pinheiro, CI Sartorão, RR Lucas, DRA Reyes, SBCV Quiroz, EMA Enriquez, RG Oliveira, JF Floriano, JPC Marcondes, S Barneze, TD Dangió, T Pascon, P Rossignoli, JV Freitas, L Takano, F Reis, TD Caldeirão, JN Fernandes, AM Carr, MVC Gaitero, JE Corrente, HRC Nunes, AF Candido, SMB Costa, TD Dangió, T Pascon, JVF Melo, L Takano, FVDS Reis, TD Caldeirão, AM Carr, GA Garcia, GB Rabadan, HCM Bassin, KS Suyama, LN Damasceno, MLS Takemoto, MD Menezes, DG Bussaneli, VKC Nogueira, PR Lima, IO Lourenço, J Marostica, RA Megid, IP Caruso, LT Rasmussen, GM Prata, F Piculo, G Vesentini, MA Arantes, GAR Ferraz, LP Camargo, MR Kron, JE Corrente, HRC Nunes Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus BMC Pregnancy Childbirth, 2020-02-19;20(1):117. 2020-02-19 [PMID: 32075598] (Human)
• S Ohta, T Ikemoto, Y Wada, Y Saito, S Yamada, S Imura, Y Morine, M Shimada A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells Sci Rep, 2019-12-10;9(1):18731. 2019-12-10 [PMID: 31822724] (Human)
• XB Hu, TT Tang, YJ Li, JY Wu, JM Wang, XY Liu, DX Xiang Phospholipid complex based nanoemulsion system for oral insulin delivery: preparation, in vitro, and in vivo evaluations Int J Nanomedicine, 2019-05-01;14(0):3055-3067. 2019-05-01 [PMID: 31118622] (Rat)
• SM Curry, ER Burrough, KJ Schwartz, KJ Yoon, SM Lonergan, NK Gabler Porcine epidemic diarrhea virus reduces feed efficiency in nursery pigs J. Anim. Sci., 2018-02-15;0(0):. 2018-02-15 [PMID: 29378029] (Porcine)
• M Philippe, H Gatterer, EM Eder, A Dzien, M Somavilla, A Melmer, C Ebenbichle, T Müller, M Burtscher The Effects of 3 Weeks of Uphill and Downhill Walking on Blood Lipids and Glucose Metabolism in Pre-Diabetic Men: A Pilot Study J Sports Sci Med, 2017-03-01;16(1):35-43. 2017-03-01 [PMID: 28344449] (Human)
• Yan J, Mei F, Cheng H, Lao D, Hu Y, Wei J, Patrikeev I, Hao D, Stutz S, Dineley K, Motamedi M, Hommel J, Cunningham K, Chen J, Cheng X Enhanced leptin sensitivity, reduced adiposity, and improved glucose homeostasis in mice lacking exchange protein directly activated by cyclic AMP isoform 1. Mol Cell Biol, 2012-12-21;33(5):918-26. 2012-12-21 [PMID: 23263987] (Mouse)

FAQs for Insulin (DINS00). (Showing 1 - 2 of 2 FAQs).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: INS