HSPA2 Antibody - BSA Free

Western Blot: HSPA2 Antibody [NBP2-16897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.

Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP2-16897] - HSPA2 antibody detects HSPA2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSPA2 antibody diluted at 1:500.

Western Blot: HspA2 Antibody [NBP2-16897] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: HspA2 Antibody [NBP2-16897] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: HSPA2 Antibody [NBP2-16897] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.

Western Blot: HSPA2 Antibody [NBP2-16897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.

Immunohistochemistry-Paraffin: HspA2 Antibody [NBP2-16897] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSPA2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSPA2. The exact sequence is proprietary.
• Predicted Species: Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HSPA2 Antibody - BSA Free

• Heat shock 70 kDa protein 2
• heat shock 70kD protein 2
• heat shock 70kDa protein 2
• heat shock-related 70 kDa protein 2
• HSP70-2
• HSP70-3
• HSPA2

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HSPA2
• Uniprot:
  • P54652(Human)