alpha-Sarcoglycan Antibody (JA51-81)

Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Western blot analysis of alpha-Sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: human skeletal muscle tissue lysate

Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-Fr, IHC-P, IP
• Clone: JA51-81
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

alpha-Sarcoglycan Antibody (JA51-81) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human alpha-Sarcoglycan aa 338-387 / 387. (SwissProt: Q16586 Human; SwissProt: P82350 Mouse; SwissProt: Q5SWB2 Rat)
• Localization: Sarcolemma, cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SGCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Application Notes: Alpha-Sarcoglycan Antibody (JA51-81) validated for IHC-Fr from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for alpha-Sarcoglycan Antibody (JA51-81)

• 50-DAG
• Adhalin
• ADL
• alphaSarcoglycan
• alpha-Sarcoglycan
• DAG2
• DMDA2
• DMDA250kD DAG
• Dystroglycan-2
• LGMD2D
• LGMD2Dalpha (50kD dystrophin-associated glycoprotein)
• sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
• SCARMD1
• SGCA

Background

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for alpha-Sarcoglycan Antibody (NBP2-67150) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 10/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Skeletal muscle tissue
• Species: Human
• Lot: HK0213

Bioinformatics

• Gene Symbol: SGCA