![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg "Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IHC-Fr, IHC-P, IP |
| Clone | JA51-81 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant Protein |
| Localization | Cell membrane. Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SGCA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Alpha-Sarcoglycan Antibody (JA51-81) validated for IHC-Fr from a verified customer review. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH 7.4), 1% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![RFC1 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "RFC1 Antibody [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Margherita verardo |
IHC-Fr | Human | 10/08/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for alpha-Sarcoglycan Antibody (NBP2-67150)Discover more about diseases related to alpha-Sarcoglycan Antibody (NBP2-67150).
| Pathways for alpha-Sarcoglycan Antibody (NBP2-67150)View related products by pathway.
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PTMs for alpha-Sarcoglycan Antibody (NBP2-67150)Learn more about PTMs related to alpha-Sarcoglycan Antibody (NBP2-67150).
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| Gene Symbol | SGCA |
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg "Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg "Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Analysis in human cerebral cortex and tonsil tissues. Corresponding ATP6V0A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0015.jpg)
![Western Blot: ATP6V0A1 Antibody [NBP1-89342] - In Cln1-/- mice V0a1 is misrouted to plasma membrane preventing its interaction with AP-3. Pull-down assay with AP-3 antibody using total lysates from untreated (lane 1) and bromopalmitate-treated (lane 2) WT brain slices to detect V0a1 and its densitometric quantitation (n=4, *P<0.05). HEK-293 cells were transfected with WT GFP-V0a1 and GFP-V0a1-Cys25Ser mutant construct, and pull-down experiments were conducted with AP-3 antibody to detect GFP-V0a1,*P<0.05(n=4). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms14612), licensed under a CC-BY licence.](http://images.novusbio.com/images2/ATP6V0A1 Antibody-Western Blot-NBP1-89342-img0018.jpg)
![Western Blot: ATP6V0A1 Antibody [NBP1-89342] - In Cln1-/- mice V0a1 is misrouted to plasma membrane preventing its interaction with AP-3. Western blot analysis and densitometric quantitation of V0a1 in isolated plasma membrane fraction from WT and Cln1-/- mouse brain (n=4, *P<0.05). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms14612), licensed under a CC-BY licence.](http://images.novusbio.com/images2/ATP6V0A1-Antibody-Western-Blot-NBP1-89342-img0017.jpg)
![Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Western-Blot-NBP2-73857-img0001.jpg)
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human prostate tissue.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.](http://images.novusbio.com/images2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73857-img0002.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)---Azide-and-BSA-Free-Western-Blot-NBP2-73859-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.](http://images.novusbio.com/images2/RFC4 Antibody (OTI1A8) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-73859-img0006.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/RFC4-Antibody-(OTI1A8)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73859-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PLEKHM1 Antibody [NBP2-38449] - Staining of human cell line U-2 OS shows localization to nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38449-img0003.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human small intestine shows moderate cytoplasmic postivity in glandular cells.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0008.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human cerebral cortex shows moderate postivity in neuropil.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/TCIRG1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89333-img0007.jpg)
![Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/images2/TCIRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89333-img0006.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/images2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Western Blot: CD20 Antibody (L26) [NBP2-44743] - Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).](http://images.novusbio.com/images2/CD20-Antibody-(L26)-Western-Blot-NBP2-44743-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD20 Antibody (L26) [NBP2-44743] - Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/images2/CD20-Antibody-(L26)-Immunocytochemistry-Immunofluorescence-NBP2-44743-img0004.jpg)
![Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (L26) [NBP2-44743] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26)](http://images.novusbio.com/images2/MS4A1-CD20-Antibody-(L26)-Immunohistochemistry-Paraffin-NBP2-44743-img0001.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/SPANXB1 Antibody-Western Blot-H00728695-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.](http://images.novusbio.com/images/Thromboxane synthase Antibody (2C1)-Western Blot-NBP2-02710-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.](http://images.novusbio.com/images/Thromboxane synthase Antibody (2C1)-Immunocytochemistry-Immunofluorescence-NBP2-02710-img0007.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/images/Thromboxane synthase Antibody (2C1)-Immunohistochemistry-Paraffin-NBP2-02710-img0006.jpg)
![Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Lane 1 mouse Heart; 2 mouse Liver (expected negative); 3 mouse Liver; 4 mouse Quadriceps; 5 mouse Triceps; 6 mouse Triceps; 7 mouse Triceps. All lanes 25ug mouse protein. Image from verified customer review.](http://images.novusbio.com/images2/gamma-Sarcoglycan-Antibody-Western-Blot-NBP1-59744-img0003.jpg)
![Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-59744] - Mouse tibialis anterior frozen sections. Image from verified customer review.](http://images.novusbio.com/images2/gamma-Sarcoglycan-Antibody-Immunohistochemistry-Frozen-NBP1-59744-img0004.jpg)
![Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Human Heart lysate, concentration 1.25ug/ml.](http://images.novusbio.com/images2/gamma Sarcoglycan Antibody-Western Blot-NBP1-59744-img0002.jpg)
![Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-NBP2-13075-img0014.jpg)
![Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Western-Blot-NBP2-13075-img0017.jpg)
![Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Western-Blot-NBP2-13075-img0016.jpg)
![Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Western-Blot-NBP2-02042-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Immunocytochemistry-Immunofluorescence-NBP2-02042-img0003.jpg)
![Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Flow-Cytometry-NBP2-02042-img0001.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human colon.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human kidney shows high expression.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0006.jpg)
![SDS-Page: Recombinant Human PLP2 Protein [H00005355-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005355-P01-1.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0009.jpg)
![Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human colon.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Immunohistochemistry-Paraffin-NBP1-88527-img0016.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0007.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")