![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg "Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, IHC, IHC-Fr, IHC-P, IP |
| Clone | JA51-81 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant Protein |
| Localization | Cell membrane. Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SGCA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Alpha-Sarcoglycan Antibody (JA51-81) validated for IHC-Fr from a verified customer review. |
|
| Reviewed Applications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH 7.4), 1% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Margherita verardo |
IHC-Fr | Human | 10/08/2019 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Diseases for alpha-Sarcoglycan Antibody (NBP2-67150)Discover more about diseases related to alpha-Sarcoglycan Antibody (NBP2-67150).
| Pathways for alpha-Sarcoglycan Antibody (NBP2-67150)View related products by pathway.
|
PTMs for alpha-Sarcoglycan Antibody (NBP2-67150)Learn more about PTMs related to alpha-Sarcoglycan Antibody (NBP2-67150).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SGCA |
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg "Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg "Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.")
![Western Blot: ATP6V0A1 Antibody [NBP1-89342] - In Cln1-/- mice V0a1 is misrouted to plasma membrane preventing its interaction with AP-3. Western blot analysis and densitometric quantitation of V0a1 in isolated plasma membrane fraction from WT and Cln1-/- mouse brain (n=4, *P<0.05). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms14612), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Western-Blot-NBP1-89342-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89342-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0016.jpg)
![Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize2/RFC2 Antibody (2B2)-Western Blot-NBP2-45570-img0013.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Immunohistochemistry-NBP2-45570-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Flow-Cytometry-NBP2-45570-img0001.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize2/RFC4-Antibody-(OTI1A8)-Western-Blot-NBP2-45946-img0005.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/RFC4-Antibody-(OTI1A8)-Immunohistochemistry-NBP2-45946-img0004.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/RFC4-Antibody-(OTI1A8)-Western-Blot-NBP2-45946-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PLEKHM1 Antibody [NBP2-38449] - Staining of human cell line U-2 OS shows localization to nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38449-img0003.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human small intestine shows moderate cytoplasmic postivity in glandular cells.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0008.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human cerebral cortex shows moderate postivity in neuropil.](http://images.novusbio.com/fullsize2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/TCIRG1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89333-img0007.jpg)
![Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize2/TCIRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89333-img0006.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Western Blot: BBS9 Antibody [NBP1-88927] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Western-Blot-NBP1-88927-img0004.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/SPANXB1 Antibody-Western Blot-H00728695-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane synthase Antibody (2C1)-Western Blot-NBP2-02710-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.](http://images.novusbio.com/fullsize/Thromboxane synthase Antibody (2C1)-Immunocytochemistry-Immunofluorescence-NBP2-02710-img0007.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane synthase Antibody (2C1)-Immunohistochemistry-Paraffin-NBP2-02710-img0006.jpg)
![Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Lane 1 mouse Heart; 2 mouse Liver (expected negative); 3 mouse Liver; 4 mouse Quadriceps; 5 mouse Triceps; 6 mouse Triceps; 7 mouse Triceps. All lanes 25ug mouse protein. Image from verified customer review.](http://images.novusbio.com/fullsize2/gamma-Sarcoglycan-Antibody-Western-Blot-NBP1-59744-img0003.jpg)
![Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-59744] - Mouse tibialis anterior frozen sections. Image from verified customer review.](http://images.novusbio.com/fullsize2/gamma-Sarcoglycan-Antibody-Immunohistochemistry-Frozen-NBP1-59744-img0004.jpg)
![Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Human Heart lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize2/gamma Sarcoglycan Antibody-Western Blot-NBP1-59744-img0002.jpg)
![Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.](http://images.novusbio.com/fullsize2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-NBP2-13075-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).](http://images.novusbio.com/fullsize2/beta Amyloid Antibody (MOAB-2)-Immunocytochemistry-Immunofluorescence-NBP2-13075-img0009.jpg)
![Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.](http://images.novusbio.com/fullsize2/beta-Amyloid-Antibody-(MOAB-2)-Western-Blot-NBP2-13075-img0017.jpg)
![Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.](http://images.novusbio.com/fullsize2/DBT-Antibody-(OTI1G2)-Western-Blot-NBP2-02042-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.](http://images.novusbio.com/fullsize2/DBT-Antibody-(OTI1G2)-Immunocytochemistry-Immunofluorescence-NBP2-02042-img0003.jpg)
![Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize2/DBT-Antibody-(OTI1G2)-Flow-Cytometry-NBP2-02042-img0001.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human colon.](http://images.novusbio.com/fullsize2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0006.jpg)
![SDS-Page: Recombinant Human PLP2 Protein [H00005355-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005355-P01-1.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0009.jpg)
![Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human colon.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Immunohistochemistry-Paraffin-NBP1-88527-img0016.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0007.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")