DNA Polymerase delta, catalytic subunit Antibody - BSA Free

Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Analysis of HeLa, using NBP1-31541 at 1:200 dilution.

Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Human lung adenocarcinoma. DNA polymerase delta antibody diluted at 1:250.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE, antibody diluted at 1:3000.

Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP1-31541] - Rat2 whole cell lysate/extract.

• Reactivity: Hu, Rt, Mu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNA Polymerase delta, catalytic subunit Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA Polymerase delta, catalytic subunit. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (92%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody - BSA Free

• CDC2 homolog
• CDC2
• DNA polymerase delta catalytic subunit
• DNA polymerase subunit delta p125
• DNA-directed polymerase delta 1
• EC 2.7.7.7
• POLD
• polymerase (DNA directed), delta 1, catalytic subunit (125kD)
• polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

DNA polymerase d (delta) functions in DNA replication and DNA repair. It also possesses 3' to 5' exonuclease activity. In leading strand synthesis it requires the processivity factor, PCNA. It is also possibly involved in the completion of DNA synthesis during Okazaki fragment elongation following the initiation of synthesis by the DNA polymerase a/primase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DNA Polymerase delta, catalytic subunit Antibody (NBP1-31541)(1)

Publication using NBP1-31541

• Murdocca M, Spitalieri P, De Masi C et al. Functional analysis of POLD1 p.ser605del variant: the aging phenotype of MDPL syndrome is associated with an impaired DNA repair capacity Aging 2021-02-22 [PMID: 33618333] (WB, Human)

Bioinformatics

• Gene Symbol: POLD1
• Entrez:
  • 5424(Human)