DR3/TNFRSF25 Antibody

Images

 
Western Blot: DR3/TNFRSF25 Antibody [NBP1-77019] - Jurkat total cell lysate with DR3/TNFRSF25 antibody at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: DR3/TNFRSF25 Antibody [NBP1-77019] - Jurkat cells.
Immunocytochemistry/ Immunofluorescence: DR3/TNFRSF25 Antibody [NBP1-77019] - Jurkat cells with DR3/TNFRSF25 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DR3/TNFRSF25 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor. The immunogen is located within amino acids 50 - 100 of DR3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNFRSF25
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
DR3 antibody can be used for detection of DR3 expression by Western blot at 1:500 to 1:1000 dilution. 59 kDa band should be detected.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DR3/TNFRSF25 Peptide (NBP1-77019PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for DR3/TNFRSF25 Antibody

  • APO3
  • Apo-3
  • Apoptosis-inducing receptor AIR
  • Apoptosis-mediating receptor DR3
  • Apoptosis-mediating receptor TRAMP
  • Death receptor 3
  • DR3
  • DR3member 12 (translocatingchain-association membrane protein)
  • LARD
  • Lymphocyte-associated receptor of death
  • Protein WSL
  • Protein WSL-1
  • TNFRSF25
  • TRAMP
  • tumor necrosis factor receptor superfamily, member 25
  • WSL
  • WSL1
  • WSL-1

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-kappaB activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF25
Entrez
Uniprot