HSP70/HSPA1A [p Ser153] Antibody - Azide Free

Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773] - Western blot of T47D cell lysate treated with EGF (1nM) for 60minutes showing specific labeling of the ~70 kDa Hsp70 in thefirst lane (-). Phosphospecificity is shown in the second lane(+) where immunolabeling is eliminated or significantlydecreased with lambda phosphatase (Lambda-Ptase, 1200 units for30 minutes).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

HSP70/HSPA1A [p Ser153] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr153 conjugated to KLH. Accession # P0DMV8
• Modification: p Ser153
• Specificity: Specific for the ~70 kDa HSP70/HSPA1A phosphorylated at Ser153. Immunolabeling of HSP70/HSPA1A is completely eliminated with lambda-phosphatase treatment. Additional bands detected are thought to be other members of the Hsp70 family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for HSP70/HSPA1A [p Ser153] Antibody - Azide Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A