HSP70/HSPA1A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Western Blot: HSP70/HSPA1A Antibody [NBP2-46806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Hsp70 / Hsc70 Antibody [NBP2-46806] - Staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human prostate shows moderate nuclear positivity in a subset of glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSP70/HSPA1A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ACERAKRTLSSSTQASLEIDSL
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  **** Hsp70 / Hsc70 Recombinant Protein Antigen (NBP2-46806PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody - BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NBP2-46806)(2)

Publications using NBP2-46806

• Benjamin J. Lang, Kristina M. Holton, Martin E. Guerrero-Gimenez, Yuka Okusha, Patrick T. Magahis, Amy Shi, Mary Neguse, Shreya Venkatesh, Anh M. Nhu, Jason E. Gestwicki, Stuart K. Calderwood Heat shock protein 72 supports extracellular matrix production in metastatic mammary tumors Cell Stress & Chaperones 2024-06-01 [PMID: 38703814]
• Doigneaux C, Pedley AM, Mistry IN et al. Hypoxia Drives the Assembly of the Multi-Enzyme Purinosome Complex J. Biol. Chem. 2020-05-21 [PMID: 32439803] (WB, Human)

Bioinformatics

• Gene Symbol: HSPA1A
• Uniprot:
  • E7EP94(Human)