HSP70/HSPA1A Antibody - BSA Free

Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).

Immunohistochemistry-Paraffin: HSP70 / HSC70 Antibody [NBP1-97872] - Analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Sh, Ye
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSP70/HSPA1A Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to a portion of rat Hsp70.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:1000
• Application Notes: Use in ELISA reported in scientific literature (PMID: 31283944).

Reactivity Notes

Avian reactivity reported in scientific literature (PMID:31283944).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for HSP70/HSPA1A Antibody - BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NBP1-97872)(1)

Publication using NBP1-97872

• Shanmugasundaram R, Wick M, Lilburn MS Effect of a post-hatch lipopolysaccharide challenge in Turkey poults and ducklings after a primary embryonic heat stress Dev. Comp. Immunol. 2019-07-05 [PMID: 31283944] (ELISA, Avian)

Review for HSP70/HSPA1A Antibody (NBP1-97872) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Duck.

reviewed by: Verified Customer ELISA Duck 02/14/2018

Summary

• Application: ELISA
• Sample Tested: EDTA Plasma
• Species: Duck
• Lot: 01031728

Bioinformatics

• Gene Symbol: HSPA1A
• Uniprot:
  • Q07439(Rat)