HSP70/HSPA1A Antibody (S01-6H6)

Western Blot: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Western blot detection of HSP70/HSPA1A in Hela cell lysates using NBP3-19657 (1:1000 diluted). Predicted band size: 70kDa. Observed band size: 70kDa.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Immunohistochemistry of HSP70/HSPA1A in paraffin-embedded Human tonsil using NBP3-19657 at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S01-6H6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

HSP70/HSPA1A Antibody (S01-6H6) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of mouse HSP70/HSPA1A (Uniprot # P0DMV8)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for HSP70/HSPA1A Antibody (S01-6H6)

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A