HSP70/HSPA1A Antibody (001) - Azide and BSA Free

Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSPA1A monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000).

Flow Cytometry: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Analysis of Human HSPA1A expression on HeLa cells. The cells were treated, stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000).

Immunoprecipitation: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: 001
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSP70/HSPA1A Antibody (001) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP70/HSPA1A (Accession#: NP_005337.2; Ala2-Asp641).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-4 ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for HSP70/HSPA1A Antibody (001) - Azide and BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NBP2-89951)(1)

Publication using NBP2-89951

• El-Ayoubi A, Klawitter M, Rüttinger J et al. Intranasal Delivery of the Oncolytic Adenovirus XVir-N-31 via Optimized Shuttle Cells Significantly Extends the Survival of Glioblastoma Bearing Mice preprints.org 2023-08-22 [PMID: 37894279] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: HSPA1A