DNAJB6 Antibody (2C11-C1)

Images

 
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - DNAJB6 monoclonal antibody (M01), clone 2C11-C1 Analysis of DNAJB6 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Analysis of monoclonal antibody to DNAJB6 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - DNAJB6 monoclonal antibody (M01), clone 2C11-C1. Analysis of DNAJB6 expression in C32.
Western Blot: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody (M01), clone 2C11-C1.Lane 1: DNAJB6 transfected lysate(27 KDa).Lane 2: ...read more
ELISA: DNAJB6 Antibody (2C11-C1) [H00010049-M01] - Detection limit for recombinant GST tagged DNAJB6 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, S-ELISA
Clone
2C11-C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DNAJB6 Antibody (2C11-C1) Summary

Immunogen
DNAJB6 (AAH00177, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK
Specificity
DNAJB6 - DnaJ (Hsp40) homolog, subfamily B, member 6
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNAJB6
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Frozen
  • Sandwich ELISA
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31955980).
Publications
Read Publications using
H00010049-M01 in the following applications:

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNAJB6 Antibody (2C11-C1)

  • DJ4
  • DKFZp566D0824
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • dnaJ homolog subfamily B member 6
  • DnaJ
  • DnaJ-like 2 protein
  • FLJ42837
  • Heat shock protein J2
  • HHDJ1
  • HSJ2
  • HSJ-2
  • MGC1152
  • MRJMGC117297
  • MSJ1
  • MSJ-1

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00010049-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-Fr, S-ELISA

Publications for DNAJB6 Antibody (H00010049-M01)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-Fr, WB.


Filter By Application
IHC-Fr
(1)
WB
(4)
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Filter By Species
Human
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using H00010049-M01 Applications Species
Palmio J, Jonson PH, Inoue M et al. Mutations in the J domain of DNAJB6 cause dominant distal myopathy Neuromuscul. Disord. Nov 19 2019 [PMID: 31955980] (IHC-Fr, Human) IHC-Fr Human
Hong YM, Hong Y, Choi YG et al. The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss. Oxid Med Cell Longev 2017 Feb 9 [PMID: 28280525]
Ding Y, Long PA, Bos JM et al. A modifier screen identifies DNAJB6 as a cardiomyopathy susceptibility gene. JCI Insight 2016 Sep 08 [PMID: 27642634] (WB) WB
Bengoechea R, Pittman SK, Tuck EP et al. Myofibrillar disruption and RNA-binding protein aggregation in a mouse model of limb-girdle muscular dystrophy 1D. Hum. Mol. Genet. 2015 Sep 11 [PMID: 26362252] (WB, Human) WB Human
Stein KC, Bengoechea R, Harms MB et al. Myopathy-Causing Mutations in an Hsp40 Chaperone Disrupt Processing of Specific Client Conformers. J. Biol. Chem. 2014 Jun 11 [PMID: 24920671] (WB, Human)

Details:
Used in WB at 1:500 dilution; Figure 6 C - Extracts of HeLa cells transfected with various DNAJB6b constructs or untransfected cells/control processed for WB with TDP-43, DNAJB6b, HSP70 and GAPDH antibodies. Figure 7C - WB of DNAJB6b and GAPDH on fibroblasts from three independent disease control patients (C1, C2, C3) having sporadic amyotrophic lateral sclerosis and three different patients from the same family having the DNAJB6-F93L mutation (P1, P2, P3).
WB Human
Blandin G, Marchand S, Charton K et al. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome. Skelet Muscle. 2013 [PMID: 23414517] (WB, Human) WB Human
Edo De Bock C, Lin Z, Mekkawy AH et al. Interaction between urokinase receptor and heat shock protein MRJ enhances cell adhesion. Int J Oncol. 2010 May 01 [PMID: 20372789]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DNAJB6
Entrez
Uniprot