HSP60 Antibodies
We offer HSP60 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each HSP60 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSP60 Antibodies can be used in a variety of model species: Bacteria, Bovine, Canine, Chicken, Drosophila, Fish, Fungi, Guinea Pig, Hamster, Human, Monkey, Mouse, Parasite, Porcine, Primate, Rabbit, Rat, Reptile, Sheep, Xenopus. Use the list below to choose the HSP60 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSP60 polyclonal antibodies and browse our HSP60 monoclonal antibody catalog.
Alternate Names for HSP60 Antibodies
anti-HSP60 antibody, anti-HSPD1 antibody, anti-60 kDa chaperonin antibody, anti-Chaperonin 60 antibody, anti-CPN60 antibody, anti-GROEL antibody, anti-heat shock 60kD protein 1 (chaperonin) antibody, anti-heat shock 60kDa protein 1 (chaperonin) antibody, anti-Heat shock protein 60 antibody, anti-heat shock protein 65 antibody, anti-HLD4 antibody, anti-HSP-60 antibody, anti-HSP60SPG13 antibody, anti-HSP65 antibody, anti-HuCHA60 antibody, anti-Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial antibody, anti-P60 lymphocyte protein antibody, anti-short heat shock protein 60 Hsp60s1 antibody, anti-spastic paraplegia 13 (autosomal dominant) antibody
37 Results for "hsp60" in Primary Antibodies
HAF007). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, DA3 mouse myeloma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # MAB1800) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
HAF008). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0015.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0019.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77397] - Staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77397-img0006.jpg)
![Flow Cytometry: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Cytometry-NBP1-77397-img0014.jpg)
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1) HeLa, 2) HepG2, 3) NIH/3T3, 4) Jurkat, 5) CHO, 6) A431, 7) PC12 and 8) COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0018.jpg)
![Simple Western: HSP60 Antibody [NBP1-77397] - Lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77397-img0013.jpg)
![Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0003.jpg "Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.")
![Immunohistochemistry: HSP60 Antibody [NBP2-12734] - Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Tissue: Liver. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734).](http://images.novusbio.com/fullsize/HSP60 Antibody-Immunohistochemistry-NBP2-12734-img0004.jpg)
![Western Blot: HSP60 Antibody [NBP2-12734] - Analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0002.jpg)
![Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77396-img0007.jpg "Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77396-img0006.jpg)
![Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Intracellular-NBP1-77396-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0005.jpg)
![Simple Western: HSP60 Antibody [NBP1-77396] - Image shows a specific band for Hsp60 in 0.05 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77396-img0009.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0002.jpg "Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-NBP2-32867-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunocytochemistry-Immunofluorescence-NBP2-32867-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunohistochemistry-Paraffin-NBP2-32867-img0008.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0004.jpg)
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Mtb Rv1468c directly binds to poly-Ub chains in a UBA-dependent manner. a, b Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated w/ (+) or w/out (–) proteinase K followed by incubation w/ K632–7 (a) or K482–7 (b) poly-Ub chains at 4 °C for 4 h. c Confocal microscopy analysis for colocalization of pSC301-Mtb stably expressing green fluorescent protein (GFP) w/ K632–7 or K482–7 Ub chains in vitro. Bacteria (green) treated w/ (+) or w/out (–) proteinase K further incubated w/ poly-Ub chains as in a & b, & then immunostained using anti-Ub antibody (red). Scale bars, 10 μm. The % colocalizations of Ub & Mtb shown at the bottom. A total of 100 bacterial cells counted. Two-tailed unpaired t-test performed. d Schematic representation of Mtb Rv1468c constructs used. e In vitro interaction analysis of Ub & GST-tagged full-length Mtb Rv1468c or the truncated mutants as indicated in d. Purified recombinant GST-tagged Mtb Rv1468c variants immobilized on glutathione sepharose resin. Mono-Ub (His6-tagged), K63 or K48-linked poly-Ub (Ub2–7) added, & then the protein complexes assayed by immunoblotting w/ indicated antibodies. f Immunoblot analysis of proteins immunoprecipitated (IP) w/ anti-Flag M2 Affinity Gel from lysates of HEK293T cells transfected w/ empty vector (–) or hemagglutinin (HA)-tagged Ub (+) & Flag-tagged wild-type (WT) Rv1468c or its mutants (V64G or L65G). g Confocal microscopy analysis of HeLa cells cotransfected w/ vectors encoding HA-tagged Ub (red) & GFP-tagged WT Rv1468c, V64G or L65G mutant (green). Nuclei (blue) stained w/ DNA-binding dye DAPI. Scale bars, 10 μm. Results representatives from at least 3 independent experiments (mean ± s.e.m. of n = 5 in c). The source data used in c provided in Source Data Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31036822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32867_mouse-monoclonal-hsp60-antibody-lk2-31020241612249.jpg)
![Western Blot: HSP60 Antibody [NBP3-05536] - Western blot analysis of different cell lysates using HSP60 Antibody at a dilution of 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP3-05536-img0002.jpg "Western Blot: HSP60 Antibody [NBP3-05536] - Western blot analysis of different cell lysates using HSP60 Antibody at a dilution of 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP3-05536] - Immunofluorescent analysis of of HeLa cells stained with HSP60 Antibody at a dilution of 1:5,000 in green and costained with mouse mAb to actin at a dilution of 1:500 in red. Blue is DAPI staining of nuclear DNA. The HSP60 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05536-img0001.jpg)
![Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Western-Blot-NBP2-32973-img0010.jpg "Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973] - Immunofluorescent staining of PFA-fixed MCF-7 cells. followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunocytochemistry-Immunofluorescence-NBP2-32973-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP60 MAb (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0001.jpg)
![Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using HSP60 antibody (LK1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Flow-Cytometry-NBP2-32973-img0011.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (LK1).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0005.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0002.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0004.jpg)
![Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Western-Blot-NBP2-44758-img0008.jpg "Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of PFA-fixed MCF-7 cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Renal Cell Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of MeOH-fixed HeLa cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Bladder Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Lung Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Pancreas stained with HSP60 Monoclonal Antibody (HSPD1/780).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0003.jpg)
![Protein Array: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP60 Antibody (HSPD1/780) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Protein-Array-NBP2-44758-img0007.jpg)
![Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Western-Blot-NBP2-22440-img0015.jpg "Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0014.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in A2058 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in ATDC5 Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) shows staining in Hela Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 using a Hsp60 Monoclonal Antibody in human endothelial cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (Hsp60, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunocytochemistry-Immunofluorescence-NBP2-22440-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Kidney tissue.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunohistochemistry-Paraffin-NBP2-22440-img0004.jpg)
![Immunoprecipitation: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (HRP) at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-4B9-89-Immunoprecipitation-NBP2-22440-img0002.jpg)
![Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Western-Blot-NB120-5479-img0016.jpg "Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0013.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0014.jpg)
![Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight rotating at 4C, washed in TBST, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunoprecipitation-NB120-5479-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-89730] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89730-img0012.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-89730] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: HSP60 Antibody [NBP1-89730] - Staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-Paraffin-NBP1-89730-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: HSP60 Antibody [NBP1-89730] - Analysis using Anti-HSPD1 antibody NBP1-89730 (A) shows similar pattern to independent antibody NBP2-55503 (B).](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-89730-img0013.jpg)
