HSP60 Antibody


Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Simple Western: HSP60 Antibody [NBP1-77396] - Simple Western lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, Rt, Ha, Pm, BvSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P
1.0 mg/ml

HSP60 Antibody Summary

A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 300-360). [Swiss-Prot P10809]
Mitochondrion matrix.
Mitochondria Marker
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/ml
  • Simple Western 1:5000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
Application Notes
This Hsp60 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Human, mouse, rat, hamster, and primate. Predicted to react with bovine based on 100% sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for HSP60 Antibody

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13


Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, Hsp60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP60 Antibody (NBP1-77396) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77396AF405
Alexa Fluor 488 Labeled NBP1-77396AF488
Alexa Fluor 647 Labeled NBP1-77396AF647
Alexa Fluor 700 Labeled NBP1-77396AF700
Biotin Labeled NBP1-77396B
DyLight 405 Labeled NBP1-77396V
DyLight 488 Labeled NBP1-77396G
DyLight 550 Labeled NBP1-77396R
DyLight 650 Labeled NBP1-77396C
FITC Labeled NBP1-77396F
HRP Labeled NBP1-77396H

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Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Gene Symbol HSPD1