HSP60 Antibody - BSA Free

Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7

Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.

Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Simple Western: HSP60 Antibody [NBP1-77396] - Image shows a specific band for Hsp60 in 0.05 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Ha, Pm, Bv
• Applications: WB, Simple Western, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HSP60 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSP60 Antibody - BSA Free (NBP1-77396) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-HSP60 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 300-360). [Swiss-Prot P10809]
• Localization: Mitochondrion matrix.
• Marker: Mitochondria Marker
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:5000
  • Western Blot 0.5 ug/mL
• Application Notes: In Western blot, a band is seen at ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSP60 Antibody - BSA Free

• 60 kDa chaperonin
• Chaperonin 60
• cpn60
• GROEL
• heat shock 60kD protein 1 (chaperonin)
• heat shock 60kDa protein 1 (chaperonin)
• Heat shock protein 60
• heat shock protein 65
• HLD4
• HSP60
• HSP-60
• HSP60SPG13
• HSP65
• HSPD1
• HuCHA60
• Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
• P60 lymphocyte protein
• short heat shock protein 60 Hsp60s1
• spastic paraplegia 13 (autosomal dominant)
• SPG13

Background

Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, Hsp60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP60 Antibody (NBP1-77396)(2)

Publications using NBP1-77396

• Brandwein D Investigating 14-3-3 Protein Subcellular Localization, Colocalization with Subcellular Markers and Interaction with Rac1 Thesis (ICC/IF, Monkey, Human)
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: HSPD1
• Entrez:
  • 3329(Human)
  • 15510(Mouse)
• Uniprot:
  • P10809(Mouse)
  • P63038(Mouse)