HSD3B1 Antibody (FDO66Q) [DyLight 650]

• Reactivity: Hu, Mu, Rt, Po, Bv, Gt, Pm, Pm, Sh
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: FDO66Q
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

HSD3B1 Antibody (FDO66Q) [DyLight 650] Summary

• Immunogen: JEG Choriocarcinoma cells
• Localization: Membrane
• Marker: Trophoblast-associated Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD3B1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.

Reactivity Notes

Human, baboon, bovine, rat and marmoset.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HSD3B1 Antibody (FDO66Q) [DyLight 650]

• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
• 3-beta-HSD I
• 3BETAHSD
• 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
• 3BH
• delta-5-3-ketosteroid isomerase
• EC 1.1.1
• HSD3B
• HSDB3
• HSDB3A
• hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
• I
• progesterone reductase
• SDR11E1
• short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
• steroid Delta-isomerase
• Trophoblast antigen FDO161G

Background

3-beta hydroxysteroid dehydrogenase (HSD3B1) is a bifunctional enzyme involved in the oxidative conversion of ketosteroids that plays an important role in the synthesis of all steroid hormones. There are two HSD3B1 proteins, designated type I and type II, that are expressed by different genes and function in different areas of the body. HSD3B1 has also been shown to be a highly specific and sensitive trophoblast-associated marker (Mao et. al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD3B1