HMGA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA‑MD‑231 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HMGA2 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

HMGA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HMGA2
Ser2-Asp109
Accession # P52926
Specificity
Detects human and mouse HMGA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HMGB1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HMGA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3184 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGA2 Antibody [Unconjugated]

  • BABL
  • BABLHMGI-C
  • high mobility group AT-hook 2
  • High mobility group AT-hook protein 2
  • high mobility group protein HMGI-C
  • high-mobility group (nonhistone chromosomal) protein isoform I-C
  • High-mobility group protein HMGI-C
  • HMGA2
  • HMGIC
  • HMGICSTQTL9
  • LIPO

Background

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGA2 Antibody (AF3184)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 3 applications: IHC, IHC Paraffin-embedded, WB.


Filter By Application
IHC
(2)
IHC Paraffin-embedded
(4)
WB
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Canine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF3184 Applications Species
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
Sterenczak K, Eckardt A, Kampmann A, Willenbrock S, Eberle N, Langer F, Kleinschmidt S, Hewicker-Trautwein M, Kreipe H, Nolte I, Murua Escobar H, Gellrich N HMGA1 and HMGA2 expression and comparative analyses of HMGA2, Lin28 and let-7 miRNAs in oral squamous cell carcinoma. BMC Cancer, 2014;14(0):694. 2014 [PMID: 25245141] (IHC,, Human) IHC Human
Willenbrock, Saskia, Wagner, Siegfrie, Reimann-Berg, Nicola, Moulay, Mohammed, Hewicker-Trautwein, Marion, Nolte, Ingo, Murua Escobar, Hugo Generation and characterisation of a canine EGFP-HMGA2 prostate cancer in vitro model. PLoS ONE, 2014;9(6):e98788. 2014 [PMID: 24914948] (IHC Paraffin-embedded,, Canine) IHC Paraffin-embedded Canine
Boudoukha S, Rivera Vargas T, Dang I, Kropp J, Cuvellier S, Gautreau A, Polesskaya A MiRNA let-7g regulates skeletal myoblast motility via Pinch-2. FEBS Lett, 2014;588(9):1623-9. 2014 [PMID: 24613920] (WB,, Mouse) WB Mouse
Downregulation of HMGA2 by the pan-deacetylase inhibitor panobinostat is dependent on hsa-let-7b expression in liver cancer cell lines. Exp Cell Res, 2012;318(15):1832-43. 2012 [PMID: 22683924] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Sredni ST, Gadd S, Huang CC, Breslow N, Grundy P, Green DM, Dome JS, Shamberger RC, Beckwith JB, Perlman EJ, Subsets of very low risk wilms tumor show distinctive gene expression, histologic, and clinical features. Clin. Cancer Res., 2009;15(22):6800-9. 2009 [PMID: 19903788] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Motoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family. Clin. Cancer Res., 2008;14(8):2334-40. 2008 [PMID: 18413822] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human

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Bioinformatics

Gene Symbol HMGA2
Entrez
Uniprot