ATP6V0A2 Antibody

Images

 
Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.
Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody
Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: ATP6V0A2 Antibody [NBP1-59069] - overexpressed mouse sequences tissue
Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Application: IHC/Immunofluorescence Species+tissue/cell type:A. untransfected HeLa cellsB. transfected HeLa cellsC. mATP6V0A2 (partial) transfected HeLa cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, GP, Ye, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP6V0A2 Antibody Summary

Immunogen
Synthetic peptides corresponding to ATP6V0A2(ATPase, H+ transporting, lysosomal V0 subunit a2) The peptide sequence was selected from the N terminal of ATP6V0A2. Peptide sequence INRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKN.
Specificity
This product is specific to Subunit or Isoform: a isoform 2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V0A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against ATP6V0A2 and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for ATP6V0A2 Antibody

  • A2
  • ARCL
  • ATP6A2
  • ATP6N1D
  • ATP6V0
  • ATPase, H+ transporting, lysosomal V0 subunit a isoform 2
  • ATPase, H+ transporting, lysosomal V0 subunit a2
  • infantile malignant osteopetrosis
  • J6B7
  • Lysosomal H(+)-transporting ATPase V0 subunit a2
  • regeneration and tolerance factor
  • RTF
  • STV1
  • TJ6A2V-ATPase
  • TJ6M
  • TJ6S
  • Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
  • vacuolar proton translocating ATPase 116 kDa subunit a
  • V-ATPase 116 kDa isoform a2
  • v-ATPase 116 kDa
  • VPH1
  • V-type proton ATPase 116 kDa subunit a isoform 2
  • v-type proton ATPase 116 kDa subunit a
  • WSS

Background

The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain; ATP6V0A2 is a component of the V(0) domain.The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain; ATP6V0A2 is a component of the V(0) domain (Smith et al., 2003 [PubMed 14580332]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP6V0A2
Uniprot