HMGA1B Antibody

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

HMGA1B Antibody Summary

Immunogen
E. coli-derived recombinant human HMGA1B
Met1-Glu96
Accession # P17096
Specificity
Detects endogenous human and mouse HMGA1B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
HMGA1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGA1B Antibody

  • HMGA1
  • HMGA1B
  • HMGIY
  • HMG-R
  • HMG-Y

Background

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10‑11 kDa member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96 amino acids (aa) in length and contains three A-T hook DNA-binding domains (aa 21‑31; 42‑52; 67‑78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11 aa insertion after Pro34. In addition, one form shows an additional 56 aa substitution for aa 55‑96, while a second shows a 14 aa substitution for aa 80‑96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HMGA1
Uniprot