HMGA1B Antibody

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGA1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5956) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HMGA1B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

HMGA1B Antibody Summary

• Immunogen: E. coli-derived recombinant human HMGA1B
  Met1-Glu96
  Accession # P17096
• Specificity: Detects endogenous human and mouse HMGA1B in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HMGA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGA1B Antibody

• HMGA1
• HMGA1A
• HMGA1B
• HMGIY
• HMG-R
• HMG-Y

Background

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10‑11 kDa member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96 amino acids (aa) in length and contains three A-T hook DNA-binding domains (aa 21‑31; 42‑52; 67‑78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11 aa insertion after Pro34. In addition, one form shows an additional 56 aa substitution for aa 55‑96, while a second shows a 14 aa substitution for aa 80‑96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGA1B Antibody (AF5956)(1)

Publication using AF5956

• Nikoli? MZ, Caritg O, Jeng Q et al. Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids Elife 6/30/2017 [PMID: 28665271]

Bioinformatics

• Gene Symbol: HMGA1
• Uniprot:
  • P17096()