HMG-CoA Reductase/HMGCR Antibody (6Y9L8)

Western Blot: HMG-CoA Reductase/HMGCR Antibody (6Y9L8) [NBP3-15641] - Analysis of extracts of various cell lines, using HMG-CoA Reductase/HMGCR antibody (NBP3-15641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 6Y9L8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HMG-CoA Reductase/HMGCR Antibody (6Y9L8) Summary

• Description: Novus Biologicals Rabbit HMG-CoA Reductase/HMGCR Antibody (6Y9L8) (NBP3-15641) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HMG-CoA Reductase/HMGCR (P04035). IKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMGCR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 -1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HMG-CoA Reductase/HMGCR Antibody (6Y9L8)

• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• EC 1.1.1
• EC 1.1.1.34
• HMG-CoA Reductase
• HMGCR
• Hydroxymethylglutaryl-CoA Reductase
• LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGCR