Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.IP samples: Jurkat whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of HDAC4 protein by 4 ug HDAC4 antibody [N3C1], Internal (NBP2-16793)5 % SDS-PAGEThe immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone Deacetylase 4/HDAC4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Histone Deacetylase 4/HDAC4. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Application Notes: IHC-FR-Assay dependent
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

• HA6116
• HD4EC 3.5.1.98
• HDAC4
• HDAC-A
• HDACABDMR
• Histone Deacetylase 4
• histone deacetylase A
• KIAA0288AHO3

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793)(2)

Publications using NBP2-16793

• Srivastava P, Mishra N, Dubey S et al. Dual regulatory role of hsa-miR-122b-5p in chikungunya virus infection via interaction with CHIKV 3'-UTR and HDAC4 modulation. Journal of Virology 2025-09-16 [PMID: 40956096]
• Sirchia SM, Faversani A, Rovina D et al. Epigenetic effects of chromatin remodeling agents on organotypic cultures Epigenomics 2016-03-01 [PMID: 26949823] (IHC-P, Human)

Review for Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Liyong Zhang WB Human 08/15/2014

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: HDAC4
• Uniprot:
  • P56524(Human)