HDAC7 Antibody - Azide and BSA Free

Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa

Immunoprecipitation: HDAC7 Antibody [NBP2-98759] - HDAC7 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-HDAC7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HDAC7 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HDAC7 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HDAC7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC7
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for HDAC7 Antibody - Azide and BSA Free

• DKFZP586J0917
• EC 3.5.1.98
• HD7
• HD7a
• HDAC7
• HDAC7A
• HDAC7AFLJ99588
• histone deacetylase 7
• Histone deacetylase 7ADKFZp586J0917

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC7