HDAC5 Antibody (CL8121)

Images

 
Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of RH-30 cells using the Anti-HDAC5 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. ...read more
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Staining of human stomach cancer shows moderate to strong cytoplasmic and nuclear positivity.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HDAC5 Antibody (CL8121) [NBP2-88913] - Analysis in human cerebral cortex and liver tissues using NBP2-88913 antibody. Corresponding HDAC5 RNA-seq data are ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
CL8121
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

HDAC5 Antibody (CL8121) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: KSKEPTPGGLNHSLPQHPK
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HDAC5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for HDAC5 Antibody (CL8121)

  • Antigen NY-CO-9
  • EC 3.5.1.98
  • FLJ90614
  • HD5
  • histone deacetylase 5
  • KIAA0600
  • NY-CO-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for HDAC5 Antibody (NBP2-88913) (0)

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Secondary Antibodies

 

Isotype Controls

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Additional HDAC5 Products

Array NBP2-88913

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Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

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Bioinformatics

Gene Symbol HDAC5