HDAC4+5+9 Antibody


Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Extracts from 3T3 cell using HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody (#21517, Lane 1 and 2) and HDAC4/HDAC5/HDAC9 (phospho- Ser246/259/220) Antibody
Immunocytochemistry/ Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.
Immunohistochemistry: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody

Product Details

Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1.0 mg/ml

Order Details

HDAC4 5 9 Antibody Summary

The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of serine 246/259/220 (T-A-Sp-E-P).
HDAC4/HDAC5/HDAC9 (Ab-246/259/220) detects endogenous levels of total HDAC4/HDAC5/HDAC9 protein.
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Read Publication using
NBP1-04967 in the following applications:

  • WB
    1 publication

Reactivity Notes

This antibody is cross-reactive against Human, Mouse. Drosophila reactivity reported in scientific literature (PMID: 25329053)

Alternate Names for HDAC4 5 9 Antibody

  • HA6116
  • HD4EC
  • HDAC-A
  • histone deacetylase 4
  • histone deacetylase A
  • KIAA0288AHO3


Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC4+5+9 Antibody (NBP1-04967)(1)

We have publications tested in 1 confirmed species: Drosophila.

We have publications tested in 1 application: WB.

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