HDAC4 5 9 Antibody

Images

 
Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Extracts from 3T3 cell using HDAC4/HDAC5/HDAC9 Antibody (Lane 1 and 2) and HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody
Immunocytochemistry/ Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.
Immunohistochemistry: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HDAC4+5+9 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of serine 246/259/220 (T-A-Sp-E-P).
Specificity
HDAC4/HDAC5/HDAC9 (Ab-246/259/220) detects endogenous levels of total HDAC4/HDAC5/HDAC9 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Publications
Read Publication using
NBP1-04967 in the following applications:

  • WB
    1 publication

Reactivity Notes

This antibody is cross-reactive against Human, Mouse. Drosophila reactivity reported in scientific literature (PMID: 25329053)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC4+5+9 Antibody

  • HA6116
  • HD4EC 3.5.1.98
  • HDAC-A
  • HDACABDMR
  • histone deacetylase 4
  • histone deacetylase A
  • KIAA0288AHO3

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-22152
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
H00004205-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NB100-1669
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NBP1-87109
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-44092
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56343
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, IP
NBP2-00493
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-27202
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Other, Pm
Applications: WB, Simple Western
NB100-58824
Species: Hu, Mu
Applications: WB, ChIP, IP, PLA
H00007001-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP2-14085
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85788
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-04967
Species: Hu, Mu, Dr
Applications: WB, ICC/IF, IHC, IHC-P

Publications for HDAC4+5+9 Antibody (NBP1-04967)(1)

We have publications tested in 1 confirmed species: Drosophila.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Drosophila
(1)
All Species

Reviews for HDAC4+5+9 Antibody (NBP1-04967) (0)

There are no reviews for HDAC4+5+9 Antibody (NBP1-04967). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC4+5+9 Antibody (NBP1-04967) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HDAC4+5+9 Products

HDAC4+5+9 NBP1-04967

Bioinformatics Tool for HDAC4+5+9 Antibody (NBP1-04967)

Discover related pathways, diseases and genes to HDAC4+5+9 Antibody (NBP1-04967). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HDAC4+5+9 Antibody (NBP1-04967)

Discover more about diseases related to HDAC4+5+9 Antibody (NBP1-04967).
 

Pathways for HDAC4+5+9 Antibody (NBP1-04967)

View related products by pathway.

PTMs for HDAC4+5+9 Antibody (NBP1-04967)

Learn more about PTMs related to HDAC4+5+9 Antibody (NBP1-04967).
 

Research Areas for HDAC4+5+9 Antibody (NBP1-04967)

Find related products by research area.

Blogs on HDAC4+5+9

There are no specific blogs for HDAC4+5+9, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HDAC4 5 9 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol HDAC4
Entrez
Uniprot