TXNIP Antibody (JY2) - Azide and BSA Free

Images

 
Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.
Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).
Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population and the right pointed the ...read more
Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - An intracellular stain was performed on THP-1 cells with TXNIP Antibody (JY2) NBP1-54578AF488 (blue) and a matched isotype control (orange). Cells ...read more
Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per ...read more
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using ...read more
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using ...read more
Flow Cytometry: TXNIP Antibody (JY2) [NBP1-54578] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready, KO
Clone
JY2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

TXNIP Antibody (JY2) - Azide and BSA Free Summary

Immunogen
Human recombinant TXNIP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TXNIP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2-5 ug/0.1x10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2ug/200ul of cell extract from 5x10^6 cells
  • Knockout Validated
  • Proximity Ligation Assay
  • Western Blot 1 ug/ml
Application Notes
IHC-Fr reported in verified customer review, see for experimental details. Knockout validation (Brocker et al).
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP1-54578 in the following applications:

Publications
Read Publications using
NBP1-54578 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34576095). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for TXNIP Antibody (JY2) - Azide and BSA Free

  • HHCPA78
  • THIF
  • thioredoxin binding protein 2
  • thioredoxin interacting protein
  • Thioredoxin-binding protein 2
  • thioredoxin-interacting protein
  • upregulated by 1,25-dihydroxyvitamin D-3
  • VDUP1EST01027
  • Vitamin D3 up-regulated protein 1

Background

Vitamin D3 up-regulated protein 1 (VDUP-1), also called Thioredoxin (TRX)-interacting protein, is an endogenous inhibitor of TRX. Redox-dependent regulation of VDUP-1 by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of VDUP-1 to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that TXNIP plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready, KO

Publications for TXNIP Antibody (NBP1-54578)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: IHC, IHC-P, IP, KD, Knockout Validated, PLA, WB.


Filter By Application
IHC
(2)
IHC-P
(2)
IP
(1)
KD
(1)
Knockout Validated
(1)
PLA
(1)
WB
(16)
All Applications
Filter By Species
Human
(4)
Mouse
(13)
Rat
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NBP1-54578 Applications Species
Bronner DN. Cells on Fire: Regulation of the NLRP3 inflammasome via endoplasmic reticulum and mitochondrial crosstalk. Thesis. 2015 (WB, Mouse) WB Mouse
Brocker CN, Kim D, Melia T et al. Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress bioRxiv (WB, Knockout Validated, Mouse) WB, Knockout Validated Mouse
Tsubaki H, Mendsaikhan A, Buyandelger U et al. Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer's Disease Brains NeuroSci Mar 31 2022 (WB, IHC, Human) WB, IHC Human
Girdhar K, Thakur S, Gaur P et al. Design, synthesis, and biological evaluation of a small molecule oral agonist of the glucagon-like-peptide-1 receptor Journal of Biological Chemistry Apr 1 2022 [PMID: 35378127] (WB, Mouse) WB Mouse
Salman M, Ismael S, Lexiao L Et al. Acute Hyperglycemia Exacerbates Hemorrhagic Transformation after Embolic Stroke and Reperfusion with tPA: A Possible Role of TXNIP-NLRP3 Inflammasome Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association Nov 27 2021 [PMID: 34847489] (WB, Mouse) WB Mouse
Salman M, Ismael S, Li L Et al. Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy Pharmaceuticals (Basel, Switzerland) Sep 27 2021 [PMID: 34681207] (WB, Mouse) WB Mouse
Yin H, Wang K, Das A et al. The REDD1/TXNIP Complex Accelerates Oxidative Stress-Induced Apoptosis of Nucleus Pulposus Cells through the Mitochondrial Pathway Oxidative Medicine and Cellular Longevity Sep 22 2021 [PMID: 34603601] (IP, Human) IP Human
Lim J, Lee S, Kim W Et al. Titanium dioxide nanoparticles exacerbate allergic airway inflammation via TXNIP upregulation in a mouse model of asthma Research Square Aug 3 2021 [PMID: 34576095] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Show All 30 Publications.

Reviews for TXNIP Antibody (NBP1-54578) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Mouse and mouse brain.

Reviews using NBP1-54578:
Filter by Applications
Immunohistochemistry-Frozen
(1)
Western Blot
(2)
All Applications
Filter by Species
Mouse
(2)
Mouse and mouse brain
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen TXNIP NBP1-54578
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 02/28/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotA2

Comments

CommentsAntibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100.
Western Blot TXNIP NBP1-54578
Enlarge
4
reviewed by:
Verified Customer
Western Blot Mouse and mouse brain 08/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain cortex whole lysate,Mouse brain
SpeciesMouse and mouse brain
LotA2
Western Blot TXNIP NBP1-54578
Enlarge
5
reviewed by:
Verified Customer
Western Blot Mouse 05/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedHepa1 cells transfected with mouse TXNIP expression vector,Hepa1 whole cell lysates
SpeciesMouse
Lot036

Comments

CommentsHepa1 lysates probed with anti-TXNIP (JY2) @ 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). Loaded 30ug protein per lane.

Product General Protocols

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FAQs for TXNIP Antibody (NBP1-54578) (0)

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Secondary Antibodies

 

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Verified Customer
02/28/2019
Application: Immunohistochemistry-Frozen
Species: Mouse

Verified Customer
08/22/2018
Application: Western Blot
Species: Mouse and mouse brain

Verified Customer
05/22/2018
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol TXNIP
Entrez
Uniprot