TXNIP Antibody (JY2)

Images

 
Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Western blot analysis of TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was ...read more
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was ...read more
Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Immunoprecipitation of Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clone
JY2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TXNIP Antibody (JY2) Summary

Immunogen
Human recombinant TXNIP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TXNIP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2ug/200ul of cell extract from 5x10^6 cells
  • Proximity Ligation Assay
Publications
Read Publications using
NBP1-54578 in the following applications:

  • 1 publication
  • PLA
    1 publication
  • WB
    6 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for TXNIP Antibody (JY2)

  • HHCPA78
  • THIF
  • thioredoxin binding protein 2
  • thioredoxin interacting protein
  • Thioredoxin-binding protein 2
  • thioredoxin-interacting protein
  • upregulated by 1,25-dihydroxyvitamin D-3
  • VDUP1EST01027
  • Vitamin D3 up-regulated protein 1

Background

Vitamin D3 up-regulated protein 1 (VDUP-1), also called Thioredoxin (TRX)-interacting protein, is an endogenous inhibitor of TRX. Redox-dependent regulation of VDUP-1 by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of VDUP-1 to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that TXNIP plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TXNIP Antibody (NBP1-54578)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC-P, PLA, WB.


Filter By Application
IHC-P
(1)
PLA
(1)
WB
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-54578 Applications Species
Zaragoza-Campillo MA, Moran J. Reactive oxygen species evoked by potassium deprivation and staurosporine inactivate Akt and induce the expression of TXNIP in cerebellar granule neurons. Oxidative Medicine and Cellular Longevity. Jan 22 2017 [PMID: 28367274] (WB, Rat) WB Rat
Chen W, Wang J, Luo Y et al. Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue. J Ginseng Res 2016 Oct [PMID: 27746687]
Ahrens TD, Timme S, Ostendorp J et al. Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity. Lab. Invest. 2015 Dec 21 [PMID: 26692290] (IHC-P, PLA, WB, Human)

Details:
TXNIP antibody clone JY2 was used at 1:5000 dilution for IHC analysis of formalin-fixed paraffin-embedded tissue specimens from esophageal cancer patients. The antibody was also used for WB (1:1000 dilution) and Proximity Ligation Assay (PLA) in experiments involving esophageal cell lines Het-1A, OE21 and OE33.
IHC-P, PLA, WB Human
Bronner DN, Abuaita BH, Chen X et al. Endoplasmic Reticulum Stress Activates the Inflammasome via NLRP3- and Caspase-2-Driven Mitochondrial Damage. Immunity 2015 Sep 15 [PMID: 26341399] (WB, Mouse) WB Mouse
Wang X, Li R, Wang X, Fu Q, Ma. Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome. Neurosci Lett. 2015 Jul 23 [PMID: 26071904]
Li Y, Yang J, Chen MH et al. Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner. Pharmacol Res. 2015 Sep [PMID: 26054569]
Zhao Y, Li Q, Zhao W et al. Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium. J Ethnopharmacol. 2015 Jul 1 [PMID: 25922268]
Li Y, Li J, Li S et al. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol. Appl. Pharmacol. 2015 Mar 16 [PMID: 25791922] (WB, Mouse) WB Mouse
Song J, Li J, Hou F et al. Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells. Metab. Clin. Exp. 2014 Nov 28 [PMID: 25499441] (WB, Human) WB Human
Wu J, Xu X, Li Y et al. Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells. Eur J Pharmacol. 2014 Dec 15 [PMID: 25446924]
Show All 14 Publications.

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Bioinformatics

Gene Symbol TXNIP
Entrez
Uniprot