MITF Antibody (21D1418)

Images

 
Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.
Western Blot: MITF Antibody (21D1418) [NB100-56561] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
21D1418
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

MITF Antibody (21D1418) Summary

Immunogen
A synthetic peptide mapping to the 400-450 a.a. region of accession number O75030.
Specificity
Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed. Users are encouraged to utilize the peptide immunogen sequence to gain further information about the isoforms recognized by the antibody.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MITF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Publications
Read Publication using NB100-56561.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MITF Antibody (21D1418)

  • bHLHe32
  • bHLHe32MI
  • Class E basic helix-loop-helix protein 32
  • MI
  • microphthalmia-associated transcription factor
  • MITF
  • Waardenburg syndrome, type 2A
  • WS2A

Background

Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MITF Antibody (NB100-56561)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56561AF405
Alexa Fluor 488 Labeled NB100-56561AF488
Alexa Fluor 647 Labeled NB100-56561AF647
Alexa Fluor 700 Labeled NB100-56561AF700
Biotin Labeled NB100-56561B
DyLight 350 Labeled NB100-56561UV
DyLight 405 Labeled NB100-56561V
DyLight 488 Labeled NB100-56561G
DyLight 550 Labeled NB100-56561R
DyLight 650 Labeled NB100-56561C
DyLight 680 Labeled NB100-56561FR
DyLight 755 Labeled NB100-56561IR
HRP Labeled NB100-56561H

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Bioinformatics

Gene Symbol MITF
Entrez
Uniprot