GTF2IRD1 Antibody

Western Blot: GTF2IRD1 Antibody [NB100-61056] - Detection of Human GTF2IRD1/TFII-IRD1 on HeLa whole cell lysate using NB100-61056. GTF2IRD1/TFII-IRD1 was also immunoprecipitated by rabbit anti-GTF2IRD1/TFII-IRD1 antibody NB100-61055.

Immunohistochemistry: GTF2IRD1 Antibody [NB100-61056] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GTF2IRD/TFII-IRD1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GTF2IRD1 Antibody Summary

• Description: Novus Biologicals Rabbit GTF2IRD1 Antibody (NB100-61056) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 909 and 959 of human general transcription factor 2I repeat domain containing 1 using the numbering given in entry NP_057412.1 (GeneID 9569).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2IRD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GTF2IRD1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GTF2IRD1 Antibody

• BEN
• CREAM1
• general transcription factor II-I repeat domain-containing protein 1
• General transcription factor III
• GTF2I repeat domain containing 1
• GTF2I repeat domain-containing protein 1
• GTF3GTF2I repeat domain-containing 1
• hMusTRD1alpha1
• muscle TFII-I repeat domain-containing protein 1 alpha 1
• Muscle TFII-I repeat domain-containing protein 1
• MusTRD1
• MusTRD1/BEN
• MUSTRD1general transcription factor 3
• RBAP2WBSCR12binding factor for early enhancer
• Slow-muscle-fiber enhancer-binding protein
• USE B1-binding protein
• WBS
• WBSCR11
• Williams-Beuren syndrome chromosomal region 11 protein
• Williams-Beuren syndrome chromosomal region 12 protein
• Williams-Beuren syndrome chromosome region 11

Background

The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2IRD1
• Entrez:
  • 9569(Human)
• Uniprot:
  • Q9UHL9(Human)