TFII-I Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TFII-I Antibody [NBP1-90010] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Analysis in human cell line SH-SY5Y.

ChIP-Exo-Seq composite graph for Anti-GTF2I (NBP1-90010) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TFII-I Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRIT
• Predicted Species: Mouse (100%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2I
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TFII-I Protein (NBP1-90010PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TFII-I Antibody - BSA Free

• BAP-135,135kD
• BAP135WBS
• Bruton tyrosine kinase-associated protein 135
• BTKAP1
• DIWS
• FLJ38776
• FLJ56355
• general transcription factor II, i
• general transcription factor IIi
• general transcription factor II-I
• GTFII-I
• IB291
• SPINBTK-associated protein 135
• TFII-ISRF-Phox1-interacting protein
• WBSCR6
• Williams-Beuren syndrome chromosome region 6

Background

TFII-I encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. The exon(s) encoding 5' UTR has not been fully defined, but this gene is known to contain at least 34 exons, and its alternative splicing generates 4 transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFII-I Antibody (NBP1-90010)(1)

Publication using NBP1-90010

• Lopez-Contreras AJ, Ruppen I, Nieto-Soler M et al. A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules Cell Rep 2013-03-26 [PMID: 23545495] (WB, Human)

Review for TFII-I Antibody (NBP1-90010) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Andres J Lopez-Contreras WB Human 06/03/2013

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: GTF2I