IRE1 alpha [p Ser724, p Ser724] Antibody


Western Blot: IRE1 alpha [p Ser724, p Ser724] Antibody [NB100-2323] - Detection in Min6 cells which were treated with different concentrations of glucose for 3 hours prior to lysates preparation.
Western Blot: IRE1 alpha [p Ser724, p Ser724] Antibody [NB100-2323] - Analysis using HRP conjugate of NB100-2323. Detection of phosphorylated IRE-1 alpha using NB100-2323. Lane 1: COS-7 untransfected Lane 2: COS-7 more
IRE1 alpha [p Ser724, p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AK and AL of NB100-2323. HeLa cells were left untreated (-) or were treated with 10 mM DTT for 60 min (+) to more

Product Details

Reactivity Hu, Mu, Rt, Po, Ma, Mk, Pm, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml

IRE1 alpha [p Ser724, p Ser724] Antibody Summary

When detecting phospho-IRE1 Alpha (Ser-724) using NB100-2323, it is recommended to normalize its band intensity/immunoreactivity with total-IRE1 alpha. NB100-2324 and NB110-59971 are a couple of options for the detection of endogenous total IRE1 Alpha.
A synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. [Swiss-Prot #O75460]
p Ser724
Endoplasmic reticulum
NB100-2323 detects IRE-1 alpha when phosphorylated at Ser724 residue.
Immunogen affinity purified
Innovator's Reward
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.025% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This IRE1 pS724 antibody is useful for Western blot, ELISA, and Immunohistochemistry paraffin embedded sections.(PMID: 19264902) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24823368). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26762342)
Control Peptide
IRE1 alpha Peptide (NB100-2323PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
NB100-2323 in the following application:

Read Publications using
NB100-2323 in the following applications:

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817).


Take a look at IRE1 alpha Antibody Sampler Pack [NBP2-50067] if you want to try 25ug aliquots of phospho-IRE1 alpha (Ser724) Antibody [NB100-2323SS] and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials.

Alternate Names for IRE1 alpha [p Ser724, p Ser724] Antibody

  • EC 2.7.11
  • endoplasmic reticulum to nucleus signaling 1
  • Endoplasmic reticulum-to-nucleus signaling 1
  • ER to nucleus signalling 1
  • FLJ30999
  • hIRE1p
  • inositol-requiring 1
  • Inositol-requiring protein 1
  • IRE 1a
  • ire1 alpha p Ser724
  • ire1 alpha phosphorylation
  • IRE1a
  • ire1-alpha
  • IRE1MGC163279
  • IRE1P
  • MGC163277
  • phospho ire1 alpha
  • phospho-ire1 alpha
  • phospho-ire1a
  • p-ire1 alpha
  • protein kinase/endoribonuclease
  • serine/threonine-protein kinase/endoribonuclease IRE1


Unfolded protein response (UPR) signaling, a mechanism used by eukaryotic cells to cope ER stress. It is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptional activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of signaling to increase production of molecular chaperones involved in protein folding; and activation of signaling that leads to targeting of misfolded proteins in ER for ubiquitination and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kinase as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-terminal domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent UPR transcriptional activator. IRE1 alpha inactivation in mice has been shown to result in widespread developmental defects, leading to intra-gestational embryonic lethality and ER stress mediated cell death is responsible for several pathologies.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRE1 alpha Antibody (NB100-2323)(127)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mammal, Primate, Rabbit.

We have publications tested in 7 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 127. Show All 127 Publications.
Publications using NB100-2323 Applications Species
Takatani T, Shirakawa J, Roe MW et al. IRS1 deficiency protects Beta -cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation Sci Rep 2016 Jul 05 [PMID:27378176] (WB) WB
Jialin G, Yao Z, Cui Y et al. Spontaneous nonalcoholic fatty liver disease and ER stress in Sidt2 deficiency mice. Biochem. Biophys. Res. Commun. May 24 2016 12:00AM [PMID:27233614] (WB, Mouse) WB Mouse
Cao A, Wang L, Chen X et al. Ursodeoxycholic acid ameliorated diabetic nephropathy by attenuating hyperglycemia-mediated oxidative stress. Biol. Pharm. Bull. May 18 2016 12:00AM [PMID:27193377] (WB, Mouse) WB Mouse
Mandal A, Mandal S, Park MH. Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells. Nature Publishing Group. 2016 May 16 [PMID:27180817] (WB, Mammal) WB Mammal
Ning B, Deng M, Zhang Q et al. beta-Asarone Inhibits IRE1/XBP1 Endoplasmic Reticulum Stress Pathway in 6-OHDA-Induced Parkinsonian Rats. Neurochem Res. [PMID:27097550] (WB, Rat) WB Rat
Merlot AM, Shafie NH, Yu Y et al. Mechanism of the induction of endoplasmic reticulum stress by the anti-cancer agent, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT): Activation of PERK/eIF2alpha, IRE1alpha, ATF6 and calmodulin kinase. Biochem Pharmacol. [PMID:27059255] (WB, Human) WB Human
Park MJ, Oh KS, Nho JH et al. Asymmetric dimethylarginine (ADMA) treatment induces apoptosis in cultured rat mesangial cells via endoplasmic reticulum stress activation. Cell Biol Int. [PMID:26992443] (WB, Rat) WB Rat
Soeda J, Mouralidarane A, Cordero P et al. Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas. J Physiol Biochem. [PMID:26979740] (WB, Human) WB Human
Kang K, Tarchick MJ, Yu X et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep. [PMID:26961159] (WB, Mouse) WB Mouse
Hong MN, Nam KY, Kim KK et al. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones. [PMID:26873011] (WB, Rat) WB Rat
Show All 127 Publications.

Reviews for IRE1 alpha Antibody (NB100-2323) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB100-2323:
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Images Ratings Applications Species Date Details
reviewed by:
WB Mouse 12/12/2014


ApplicationWestern Blot
Sample TestedSee PMID 22951927


Blocking DetailsSee PMID 22951927


Detection NotesSee PMID 22951927


CommentsPublished in PMID: 22951927
Western Blot IRE1 alpha NB100-2323
reviewed by:
WB Mouse 05/27/2009


ApplicationWestern Blot
Sample TestedMouse lens tissue, Sample Amount: 40ug


Blocking DetailsBlocking Buffer: 5% TBS+BSA, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: TBS+BSA, Incubation Time: 2 hours, Incubation Temp: 24 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: ANTI-RABBIT (CALBIOCHEM), Secondary Ab Dilution Ratio: 1/20000


Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5 minutes, Specific Bands: 110 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRE1 alpha Antibody (NB100-2323). (Showing 1 - 3 of 3 FAQs).

  1. Can you please tell me the immunogen sequence of this antibody?
    • The immunogen of NB100-2323 is a synthetic peptide from an amino acids sequence surrounding the phosphorylated serine 724 of the human IRE1 alpha protein (Swiss-Prot #O75460). Unfortunately, I cannot provide any additional detail, as that is proprietary information.
  2. What is the size of the western blot band?
    • The weight observed in our in-house testing is typically 100-110kDa
  3. Can this antibody detect non-phosphorylated IRE1a?
    • NB100-2323 is phospho-specific. In all of our testing done with this antibody, we have only detected the phosphorylated IRE1a protein. There has not been any observed cross reactivity with the non-phosphorylated protein.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2323AF405
Alexa Fluor (R) 488 Labeled NB100-2323AF488
Alexa Fluor (R) 647 Labeled NB100-2323AF647
Alexa Fluor (R) 700 Labeled NB100-2323AF700
Biotin Labeled NB100-2323B
DyLight 405 Labeled NB100-2323V
DyLight 488 Labeled NB100-2323G
DyLight 550 Labeled NB100-2323R
DyLight 650 Labeled NB100-2323C
FITC Labeled NB100-2323F
HRP Labeled NB100-2323H

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Blogs on IRE1 alpha.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

IRE1 alpha (inositol-requiring enzyme 1 alpha)
The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress. UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (...  Read full blog post.

IRE1 alpha and ER Stress: Keys to Disease Progression Pathways
Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s...  Read full blog post.

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Product PDFs


Gene Symbol ERN1

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