IRE1 alpha [p Ser724] Antibody

Images

 
Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate ...read more
Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Detection in Min6 cells which were treated with different concentrations of glucose for 3 hours prior to lysates preparation.
Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Analysis using HRP conjugate of NB100-2323. Detection of phosphorylated IRE-1 alpha using NB100-2323. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ma, Mk, Pm, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

IRE1 alpha [p Ser724] Antibody Summary

Description
When detecting phospho-IRE1 Alpha (Ser-724) using NB100-2323, it is recommended to normalize its band intensity/immunoreactivity with total-IRE1 alpha. NB100-2324 and NB110-59971 can be used for the detection of endogenous total IRE1 alpha.
Immunogen
A synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. [Swiss-Prot #O75460]
Modification
p Ser724
Localization
Endoplasmic reticulum
Specificity
NB100-2323 detects IRE-1 alpha when phosphorylated at Ser724 residue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
This IRE1 pS724 antibody is useful for Western blot, ELISA, and Immunohistochemistry paraffin embedded sections (PMID: 19264902). Use in IHC-Frozen reported in scientific literature (PMID 24823368). Use in ICC reported in scientific literature (PMID 26762342).
Positive Control
IRE1 alpha Antibody (NB100-2324)
Pancreas Lysate (NB820-59443)
Epididymis Lysate (NB820-59213)
IRE1 alpha Antibody Pack (NBP2-50067)
Control Peptide
IRE1 alpha Peptide (NB100-2323PEP)
Reviewed Applications
Read 5 Reviews rated 4.4
using
NB100-2323 in the following applications:

Publications
Read Publications using
NB100-2323 in the following applications:

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.025% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Take a look at IRE1 alpha Antibody Sampler Pack [NBP2-50067] if you want to try 25ug aliquots of phospho-IRE1 alpha (Ser724) Antibody [NB100-2323SS] and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials.

Alternate Names for IRE1 alpha [p Ser724] Antibody

  • EC 2.7.11
  • endoplasmic reticulum to nucleus signaling 1
  • Endoplasmic reticulum-to-nucleus signaling 1
  • ER to nucleus signalling 1
  • FLJ30999
  • hIRE1p
  • inositol-requiring 1
  • Inositol-requiring protein 1
  • IRE 1a
  • ire1 alpha p Ser724
  • ire1 alpha phosphorylation
  • IRE1a
  • ire1-alpha
  • IRE1MGC163279
  • IRE1P
  • MGC163277
  • phospho ire1 alpha
  • phospho-ire1 alpha
  • phospho-ire1a
  • p-ire1 alpha
  • protein kinase/endoribonuclease
  • serine/threonine-protein kinase/endoribonuclease IRE1

Background

Unfolded protein response (UPR) signaling, a mechanism used by eukaryotic cells to cope ER stress. It is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptional activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of signaling to increase production of molecular chaperones involved in protein folding; and activation of signaling that leads to targeting of misfolded proteins in ER for ubiquitination and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kinase as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-terminal domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent UPR transcriptional activator. IRE1 alpha inactivation in mice has been shown to result in widespread developmental defects, leading to intra-gestational embryonic lethality and ER stress mediated cell death is responsible for several pathologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2323
Species: Hu, Mu, Rt, Po, Ma, Mk, Pm, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for IRE1 alpha Antibody (NB100-2323)(156)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mammal, Primate, Rabbit.

We have publications tested in 7 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(5)
IHC
(5)
IHC-Fr
(1)
IHC-P
(6)
IP
(2)
WB
(120)
All Applications
Filter By Species
Human
(60)
Mouse
(57)
Rat
(19)
Mammal
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 156. Show All 156 Publications.
Publications using NB100-2323 Applications Species
Ji C, Yang B, Huang SY et al. Salubrinal protects human skin fibroblasts against UVB-induced cell death by blocking endoplasmic reticulum (ER) stress and regulating calcium homeostasis. Biochem. Biophys. Res. Commun. 2017 Dec 02 [PMID: 28988108] (Human) Human
Sun J, Ren D. IER3IP1 deficiency leads to increased B-cell death and decreased B-cell proliferation Oncotarget 2017 Aug 22 [PMID: 28915629] (WB, Human) WB Human
Plaas M, Seppa K, Reimets R et al. Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration Sci Rep 2017 Aug 31 [PMID: 28860598] (IHC, Rat) IHC Rat
Zhang X, Wu WK, Xu W et al. C-X-C Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis Theranostics 2017 Aug 21 [PMID: 28824718] (WB, Human) WB Human
Zhang XH, Wang XY, Zhou ZW et al. The combination of digoxin and GSK2606414 exerts synergistic anticancer activity against leukemia in vitro and in vivo Biofactors 2017 Aug 17 [PMID: 28817203] (Human) Human
Rius B, Duran-Guell M, Flores-Costa R et al. The specialized pro-resolving lipid mediator maresin 1 protects hepatocytes from lipotoxic and hypoxia-induced endoplasmic reticulum stress FASEB J. 2017 Aug 02 [PMID: 28768719] (Mouse) Mouse
Guo H, Wang Y, Zhang X et al. Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKalpha-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Sci Rep 2017 Jul 31 [PMID: 28761152] (WB, Mouse) WB Mouse
Nakazawa H, Ikeda K, Shinozaki S et al. Burn-induced muscle metabolic derangements and mitochondrial dysfunction are associated with activation of HIF-1alpha and mTORC1: Role of protein farnesylation Sci Rep 2017 Jul 26 [PMID: 28747716] (WB, Mouse) WB Mouse
Marcassa E, Raimondi M, Anwar T et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. Mar 16 2017 [PMID: 28302665] (WB, Human) WB Human
Imbernon Piedra M. Efectos centrales de MCH sobre el metabolismo periferico. Thesis Jun 1 2016 (WB, IHC) WB, IHC
Show All 156 Publications.

Reviews for IRE1 alpha Antibody (NB100-2323) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Mouse.

Reviews using NB100-2323:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Mouse
(5)
All Species
Images Ratings Applications Species Date Details
Western Blot IRE1 alpha NB100-2323
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reviewed by:
Insu Kwon
WB Mouse 04/03/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotAO-1

Comments

Comments C57BL/6 Mice
Western Blot IRE1 alpha NB100-2323
Enlarge
reviewed by:
Kihong Lim
WB Mouse 03/15/2017
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates: Hamster, Mouse, Human, Monkey
SpeciesMouse
LotAO-1

Comments

Comments6132 Pro cells (mouse fibrosarcoma) were treated with or without 10 mM DTT for 3 hr and total lysates were prepared. The membrane blot was incubated with 5 ul of primary antibody (IRE1 alpha [p Ser724]) in 10 ml of blocker (2% BSA in TBS containing 0.05% Tween20) for 2hr at RT, followed by incubation with HRP-conjugated secondary antibody (anti-rabbit IgG H+L, from Pierce) for 1hr. The signal was visualized using chemilluminedcent substrate (Pico, from Pierce).
Western Blot IRE1 alpha NB100-2323
Enlarge
reviewed by:
INSU KWON
WB Mouse 03/07/2017
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain
SpeciesMouse

Comments

CommentsC57BL/6 mice
 
reviewed by:
Anonymous
WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22951927
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22951927

Details

Detection NotesSee PMID 22951927

Comments

CommentsPublished in PMID: 22951927
Western Blot IRE1 alpha NB100-2323
Enlarge
reviewed by:
Anonymous
WB Mouse 05/27/2009
View

Summary

ApplicationWestern Blot
Sample TestedMouse lens tissue, Sample Amount: 40ug
SpeciesMouse
LotI

Blocking

Blocking DetailsBlocking Buffer: 5% TBS+BSA, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: TBS+BSA, Incubation Time: 2 hours, Incubation Temp: 24 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: ANTI-RABBIT (CALBIOCHEM), Secondary Ab Dilution Ratio: 1/20000

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5 minutes, Specific Bands: 110 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRE1 alpha Antibody (NB100-2323). (Showing 1 - 3 of 3 FAQs).

  1. Can you please tell me the immunogen sequence of this antibody?
    • The immunogen of NB100-2323 is a synthetic peptide from an amino acids sequence surrounding the phosphorylated serine 724 of the human IRE1 alpha protein (Swiss-Prot #O75460). Unfortunately, I cannot provide any additional detail, as that is proprietary information.
  2. What is the size of the western blot band?
    • The weight observed in our in-house testing is typically 100-110kDa
  3. Can this antibody detect non-phosphorylated IRE1a?
    • NB100-2323 is phospho-specific. In all of our testing done with this antibody, we have only detected the phosphorylated IRE1a protein. There has not been any observed cross reactivity with the non-phosphorylated protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2323AF405
Alexa Fluor 488 Labeled NB100-2323AF488
Alexa Fluor 647 Labeled NB100-2323AF647
Alexa Fluor 700 Labeled NB100-2323AF700
Biotin Labeled NB100-2323B
DyLight 405 Labeled NB100-2323V
DyLight 488 Labeled NB100-2323G
DyLight 550 Labeled NB100-2323R
DyLight 650 Labeled NB100-2323C
FITC Labeled NB100-2323F
HRP Labeled NB100-2323H

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Blogs on IRE1 alpha.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

IRE1 alpha (inositol-requiring enzyme 1 alpha)
The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress. UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (...  Read full blog post.

IRE1 alpha and ER Stress: Keys to Disease Progression Pathways
Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s...  Read full blog post.

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Recent Reviews

4.4
5
2
4
3
3
0
2
0
1
0

Insu Kwon
04/03/2017
Application: WB
Species: Mouse

Kihong Lim
03/15/2017
Application: WB
Species: Mouse

INSU KWON
03/07/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ERN1