IRE1 alpha [p Ser724] Antibody - BSA Free

P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license.

Hepatic ER stress markers with cachexia progression. ER stress markers Bip, IRE1a, ATF6p50 and CHOP were examined in the liver of non, pre and severely cachectic mice. (n = 6-8 per group, p < 0.05) Dotted line indicates levels of Non-cachectic mice. Non = Non-Cachectic Apc Min/+ Sev = severely cachectic Apc Min/+; * denotes significantly different from Non-cachectic Apc Min/+ Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0119888) licensed under a CC-BY license.

IRF-1 plays a central role in ER stress-mediated modulation of VCAM-1 expression by TGRL. HAEC were conditioned for 4 hr with TNFa (0.3 ng/ml) E: CHOP knockdown decreased TNFa-induced VCAM-1 expression. n=4. **P<0.01 vs. siCtrl+TNFa. Shown are representative blots from 3 independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0078322) licensed under a CC-BY license.

Analysis using HRP conjugate of NB100-2323. Detection of phosphorylated IRE-1 alpha using NB100-2323. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE1 alpha. Theoretical molecular weight: 110 kDa.

Analysis of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Theoretical molecular weight: 110 kDa.

Regulation of the unfolded protein response by KLF15. Western analysis of UPR activity in WT versus KLF15-/- primary hepatocytes. Hepatocytes were isolated from standard chow-fed 4-month-old male WT and KLF15 -/- mice. Two individual experiments were performed in triplicate; each lane indicates a technical replicate. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0077851) licensed under a CC-BY license.

HeLa cell lysate, 20 ug. Antibody at 1:1000, 1% skim milk in TBST, overnight incubation at 4C. WB image submitted by a verified customer review.

Detection in Min6 cells which were treated with different concentrations of glucose for 3 hours prior to lysates preparation.

IRE1 (pS724) was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human IRE1 (pS724) polyclonal Antibody (Catalog # NB100-2323) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the perinuclear cytoplasm in splenocytes.

• Reactivity: Hu, Mu, Rt, Po, Dr, Gt, Ma, Pm, Pm, Rb, SyHa
• Applications: WB, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IRE1 alpha [p Ser724] Antibody - BSA Free Summary

• Description: When detecting phospho-IRE1 Alpha (Ser-724) using NB100-2323, it is recommended to normalize its band intensity/immunoreactivity with total-IRE1 alpha. NB100-2324 and NB110-59971 can be used for the detection of endogenous total IRE1 alpha.
• Immunogen: This IRE1 alpha [p Ser724] antibody was raised against a synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. [Swiss-Prot #O75460]
• Modification: p Ser724
• Localization: Endoplasmic reticulum
• Specificity: NB100-2323 IRE1 alpha [p Ser724] Antibody detects IRE-1 alpha when phosphorylated at Ser724 residue.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100 - 1:2000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Knockdown Validated
  • Western Blot 1:500 - 1:1000
• Application Notes: This IRE1 pS724 antibody is useful for WB, ELISA, and IHC-P sections (PMID: 19264902). Use in IHC-Frozen reported in scientific literature (PMID: 24823368). Use in ICC reported in scientific literature (PMID: 26762342). Use in immunoblotting reported in scientific literature (PMID: 24089213). Use In vitro assay reported in scientific literature (PMID: 24327956). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 25225294). Knockdown Validated reported in scientific literature (PMID: 31159306).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  IRE1 alpha Antibody
  IRE1 alpha Antibody
  Human Pancreas Whole Tissue Lysate (Adult Whole Tumor)
  Human Epididymis Whole Tissue Lysate (Adult Whole Normal)
  IRE1 alpha Antibody Pack
  HeLa DTT Treated / Untreated Cell Lysate

• Control Peptide:

  IRE1 alpha Protein (NB100-2323PEP)

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:35492579).Drosophila reactivity reported in scientific literature (PMID: 31641108). Goat reactivity reported in scientific literature (PMID: 29046053). Use in Golden Syrian Hamster reported in scientific literature (PMID:31167774).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Take a look at IRE1 alpha Antibody Sampler Pack [NBP2-50067] if you want to try 25ug aliquots of phospho-IRE1 alpha (Ser724) Antibody [NB100-2323SS] and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials.

Alternate Names for IRE1 alpha [p Ser724] Antibody - BSA Free

• EC 2.7.11
• endoplasmic reticulum to nucleus signaling 1
• Endoplasmic reticulum-to-nucleus signaling 1
• ER to nucleus signalling 1
• ERN1
• FLJ30999
• hIRE1p
• inositol-requiring 1
• Inositol-requiring protein 1
• IRE 1a
• ire1 alpha p Ser724
• ire1 alpha phosphorylation
• IRE1 alpha
• IRE1
• IRE1a
• Ire1-alpha
• IRE1MGC163279
• IRE1P
• MGC163277
• phospho ire1 alpha
• phospho-ire1 alpha
• phospho-ire1a
• p-ire1 alpha
• protein kinase/endoribonuclease
• serine/threonine-protein kinase/endoribonuclease IRE1

Background

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).

Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.

IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

References

1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3

2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0

3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y., . . . Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRE1 alpha Antibody (NB100-2323)(296)

Publications using NB100-2323

• Cui J, Xu H, Shi J et al. Carbonic anhydrase IX inhibitor S4 triggers release of DAMPs related to immunogenic cell death in glioma cells via endoplasmic reticulum stress pathway Cell communication and signaling : CCS 2023-06-29 [PMID: 37386564] (WB, Human)
• Zhou F, Gao H, Shang L et al. Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer Journal of experimental & clinical cancer research : CR 2023-06-19 [PMID: 37337284] (WB, Human)
• Reut D, Jhee H, Caroline B et al. Neuronal IRE-1 coordinates an organism-wide cold stress response by regulating fat metabolism Cell reports 2022-11-29 [PMID: 36450261] (WB)
• Wang W, Hawkridge AM, Ma Y et al. Ubiquitin-like protein 5 is a novel player in the UPR-PERK arm and ER stress-induced cell death The Journal of biological chemistry 2023-06-12 [PMID: 37315790] (WB, Mouse)
• Varghese D, Oommen D, John A, Ali B GRP78/BiP alleviates oxLDL-induced hepatotoxicity in familial hypercholesterolemia caused by missense variants of LDLR Research Square 2023-02-24 [PMID: 37248472] (WB, Human)
• Xiao J, Huang J, Jian X et al. IRE1? arm of unfolded protein response in muscle-specific TGF-? signaling-mediated regulation of muscle cell immunological properties Cellular & molecular biology letters 2023-02-27 [PMID: 36849929] (IHC, WB, Mouse)
• Yu J, Shen S, Yan Y et al. Iodide Excess Inhibits Thyroid Hormone Synthesis Pathway Involving XBP1-Mediated Regulation Nutrients 2023-02-09 [PMID: 36839245] (WB, Mouse)
• Yu D, Li J, Wang Y et al. Oridonin ameliorates acetaminophen-induced acute liver injury through ATF4/PGC-1 alpha pathway Drug development research 2022-12-26 [PMID: 36567664]
• Rahman MK, Umashankar B, Choucair H et al. Inclusion of the in-chain sulfur in 3-thiaCTU increases the efficiency of mitochondrial targeting and cell killing by anticancer aryl-urea fatty acids European journal of pharmacology 2023-01-15 [PMID: 36543287] (WB, Human)
• Madhamanchi K, Madhamanchi P, Jayalakshmi S et al. Endoplasmic reticulum stress and unfolded protein accumulation correlate to seizure recurrence in focal cortical dysplasia patients Cell stress & chaperones 2022-10-18 [PMID: 36258150]
• Pritchard KA, Jing X, Teng M et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia PloS one 2022-08-26 [PMID: 36018859] (IHC-P, WB, Human, Rat)
• Peng D, Wang YX, Huang TH et al. Ligustilide Improves Cognitive Impairment via Regulating the SIRT1/IRE1 alpha/XBP1s/CHOP Pathway in Vascular Dementia Rats Oxidative medicine and cellular longevity 2022-08-16 [PMID: 36017237] (IHC-P, WB, Rat)
• Jian Z, Han Y, Zhang W et al. Anti-tumor effects of dual PI3K-HDAC inhibitor CUDC-907 on activation of ROS-IRE1 alpha-JNK-mediated cytotoxic autophagy in esophageal cancer Cell & bioscience 2022-08-21 [PMID: 35989326] (IHC-P, Human)
  
  Details:
  Dilutions: 1:500
• Soto I, Couvillion M, Hansen KG et al. Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes Genome biology 2022-08-09 [PMID: 35945592] (WB)
• Lei X, Lin H, Wang J et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression Nature communications 2022-07-06 [PMID: 35794100] (WB, Human)
• Li J, Lei X, Ruan Y et al. Inhibition of Mitochondrial Fission Reverses the Immunoescape of Solid Tumors via IRE1 alpha-XBP-1s-TPP2 axis Research Square 2020-08-14 (WB, Mouse, Human)
• Chen Y, Zhang T, Zeng S et al. Transketolase in human Muller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways Redox Biology 2022-08-01 [PMID: 35779441] (WB, Human)
• Chen JH, Wu CH, Jheng JR et al. The down-regulation of XBP1, an unfolded protein response effector, promotes acute kidney injury to chronic kidney disease transition Journal of biomedical science 2022-06-28 [PMID: 35765067] (WB, Mouse)
• Chakraborty S, Basu A miR-451a Regulates Neuronal Apoptosis by Modulating 14-3-3 zeta-JNK Axis upon Flaviviral Infection mSphere 2022-06-21 [PMID: 35727063] (WB, Mouse)
• Fanning S, Cirka H, Thies JL et al. Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy NPJ Parkinson's disease 2022-06-09 [PMID: 35680956]
• Wang X, Xin H, Xing M et al. Acute Endoplasmic Reticulum Stress Induces Inflammation Reaction, Complement System Activation, and Lipid Metabolism Disorder of Piglet Livers: A Proteomic Approach Frontiers in Physiology 2022-04-13 [PMID: 35492579] (WB, Porcine)
• Li Q, Fan X, Lu W et al. Novel NPR2 Gene Mutations Affect Chondrocytes Function via ER Stress in Short Stature Cells 2022-04-08 [PMID: 35455946] (WB)
• Visa, A;Alza, L;CantI, C;Herreros, J; Tetralol derivative NNC-55-0396 induces glioblastoma cell death by activating IRE1alpha, JNK1 and calcium signaling Biomedicine & Pharmacotherapy [PMID: 35367758]
• Lu H, Gomaa A, Wang-Bishop L et al. Unfolded Protein Response Is Activated by Aurora Kinase A in Esophageal Adenocarcinoma Cancers 2022-03-09 [PMID: 35326553] (IHC-P, Human)
• Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology 2022-01-05 [PMID: 34984929] (WB, Mouse)
• Abdullah CS, Aishwarya R, Alam S et al. The molecular role of Sigmar1 in regulating mitochondrial function through mitochondrial localization in cardiomyocytes Mitochondrion 2022-01-01 [PMID: 34902622] (WB, Rat, Mouse, Human)
• Tanaka K, Kandori S, Sakka S Et al. ELOVL2 promotes cancer progression by inhibiting cell apoptosis in renal cell carcinoma Oncology reports 2022-02-01 [PMID: 34841437] (WB, Human)
• Xia H, Zhao H, Yang W Et al. MiR-195-5p represses inflammation, apoptosis, oxidative stress, and endoplasmic reticulum stress in sepsis-induced myocardial injury by targeting activating transcription factor 6 Cell biology international 2021-11-24 [PMID: 34816499] (WB, Mouse)
• Li S, Zhao F, Tang Q Et al. Sarco/endoplasmic reticulum Ca2+ -ATPase 2b mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain British journal of pharmacology 2021-11-22 [PMID: 34811737]
• Fan Y, Wolford B, Lu H Et al. Type 2 diabetes sex-specific effects associated with E167K coding variant in TM6SF2 iScience 2021-11-01 [PMID: 34746691] (WB, Mouse)
• Venkatesh P, Kumar S, Kumar N, et al. Molecular mechanisms underlying attenuation of live attenuated Japanese encephalitis virus vaccine SA14-14-2 bioRxiv 2021-01-15 (WB, Mouse, Porcine)
• Chunhacha P, Pinkaew D, Sinthujaroen P Et al. Fortilin inhibits p53, halts cardiomyocyte apoptosis, and protects the heart against heart failure Cell death discovery 2021-10-23 [PMID: 34689154] (WB, Mouse)
• Khoi CS, Lin YW, Chen JH Et al. Selective Activation of Endoplasmic Reticulum Stress by Reactive-Oxygen-Species-Mediated Ochratoxin A-Induced Apoptosis in Tubular Epithelial Cells International journal of molecular sciences 2021-10-11 [PMID: 34681610] (WB, Human)
• Mareninova OA, Dillon DL, Wightman CJM Et al. Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis Cellular and molecular gastroenterology and hepatology 2021-10-02 [PMID: 34610499]
• Shaban MS, MUller C, Mayr-Buro C et al. Multi-level inhibition of coronavirus replication by chemical ER stress Nature communications 2021-09-20 [PMID: 34545074] (WB)
• Zhao P, Huang P, Xu T Et Al. Fat body Ire1 regulates lipid homeostasis through the Xbp1s-FoxO axis in Drosophila iScience 2021-08-01 [PMID: 34381963]
• Einstein Jm, Perelis M, Chaim Ia Et Al. Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer Molecular cell 2021-06-29 [PMID: 34216543] (WB)
• Witte F, Ruiz-Orera J, Mattioli C et al. A trans locus causes a ribosomopathy in hypertrophic hearts that affects mRNA translation in a protein length-dependent fashion Genome Biology 2021-12-01 [PMID: 34183069] (WB, Rat)
• Zhou Y, Ye X, Zhang C et al. Ufl1 deficiency causes kidney atrophy associated with disruption of endoplasmic reticulum homeostasis Journal of Genetics and Genomics 2021-05-01 [PMID: 34148841]
• Ricci D, Tutton S, Marrocco I et al. An interdomain helix in IRE1 alpha mediates the conformational change required for the sensor's activation The Journal of biological chemistry 2021-05-14 [PMID: 34000298] (WB, Human)
• Zhemkov V, Ditlev JA, Lee WR et al. The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains eLife 2021-05-11 [PMID: 33973848] (WB, Human)
• Zhang Y, Wu N, Gan-Schreier H et al. FATP4 inactivation in cultured macrophages attenuates M1- and ER stress-induced cytokine release via a metabolic shift towards triacylglycerides The Biochemical journal 2021-04-26 [PMID: 33900381]
• Cao X, Shu Y, Chen Y et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis Cellular and molecular gastroenterology and hepatology 2021-04-10 [PMID: 33848642] (WB, Mouse)
• Rigby MJ, Lawton AJ, Kaur G et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism Communications biology 2021-04-12 [PMID: 33846551] (WB, Mouse)
• Lim Y, Kim S, Kim EK Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells Molecular brain 2021-04-06 [PMID: 33823883]
• Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports 2021-04-01 [PMID: 33795715] (WB, Mouse)
• Parthibane V, Lin J, Acharya D et al. SSSPTA is essential for serine palmitoyltransferase function during development and hematopoiesis Journal of Biological Chemistry 2021-03-01 [PMID: 33662400] (WB, Mouse)
• Chen N, Zhang Y, Wang M et al. Maternal Obesity Interrupts the Coordination of the Unfolded Protein Response and Heat Shock Response in the Postnatal Developing Hypothalamus of Male Offspring in Mice Molecular and cellular endocrinology 2021-02-23 [PMID: 33636254] (WB, Mouse)
• Cao X, An J, Cao Y et al. EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development Investigative ophthalmology & visual science 2021-02-01 [PMID: 33605987] (ICC/IF, WB, Mouse)
• De Nisco NJ, Casey AK, Kanchwala M et al. Manipulation of IRE1-Dependent MAPK Signaling by a Vibrio Agonist-Antagonist Effector Pair mSystems 2021-02-09 [PMID: 33563785] (WB, Mouse)
• Wanschel ACBA, Guizoni DM, Lorza-Gil E, et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction Biomolecules 2021-01-07 [PMID: 33430172]
• Zhou L, Song Z, Hu J et al. ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3 Theranostics 2020-11-06 [PMID: 33391508] (WB, Human)
• Kalish BT, Kim E, Finander B et al. Maternal immune activation in mice disrupts proteostasis in the fetal brain Nature neuroscience 2020-12-23 [PMID: 33361822]
• Bontemps-Karcher A, Magneron V, Conquet L et al. Renal adaptive response to exposure to low doses of uranyl nitrate and sodium fluoride in mice Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2020-12-17 [PMID: 33360916] (IHC-P, Mouse)
• Arioka Y, Shishido E, Kushima I et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons EBioMedicine 2020-12-17 [PMID: 33341442] (WB, Human)
• Lanzillotta C, Greco V, Valentini D et al. Proteomics Study of Peripheral Blood Mononuclear Cells in Down Syndrome Children Stem Cells 2020-11-07 [PMID: 33187268] (WB, Mouse)
• O'Brien CE, Santos PT, Kulikowicz E et al. Neurologic effects of short-term treatment with a soluble epoxide hydrolase inhibitor after cardiac arrest in pediatric swine Elife 2020-10-19 [PMID: 33129262]
• Mello A, Hsu MF, Koike S et al. Soluble epoxide hydrolase hepatic deficiency ameliorates alcohol-associated liver disease Cell Mol Gastroenterol Hepatol 2020-10-14 [PMID: 33068774] (WB, Mouse)
• Xiong Z, Yuan C, Shi J et al. Restoring the epigenetically silenced PCK2 suppresses renal cell carcinoma progression and increases sensitivity to sunitinib by promoting endoplasmic reticulum stress Theranostics 2020-09-15 [PMID: 33052225] (WB, Human)
• Chen Y, Griffiths A, Wang J et al. Inositol requiring enzyme 1 alpha (IRE1a) links palmitate-induced mTOR activation and lipotoxicity in Hepatocytes Am J Physiol Cell Physiol 2020-10-14 [PMID: 33052067]
• Kim H, Cho Y, Kim HS et al. A system-level approach identifies HIF-2 alpha as a critical regulator of chondrosarcoma progression Nat Commun 2020-10-06 [PMID: 33024108] (WB, Rat, Human)
• Ding R, Ou W, Chen C et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation Neurochem. Int. 2020-09-24 [PMID: 32980492] (WB, Rat)
• Zhao Q, Zhong J, Bi Y et al. Gambogenic acid induces Noxa-mediated apoptosis in colorectal cancer through ROS-dependent activation of IRE1alpha/JNK Phytomedicine 2020-01-01 [PMID: 32854039]
• Lanzillotta C, Zuliani I, Tramutola A et al. Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention Prog. Neurobiol. 2020-08-11 [PMID: 32795489] (WB, Mouse, Human)
• Aryal YP, Lee ES, Kim TY et al. Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development Gene Expr. Patterns 2020-08-03 [PMID: 32758541] (IHC-P, Mouse)
• Ganesan M, Mathews S, Makarov E et al. Acetaldehyde Suppresses HBV-MHC Class I Complex Presentation on Hepatocytes Via Induction of ER Stress and Golgi Fragmentation Am. J. Physiol. Gastrointest. Liver Physiol. 2020-08-05 [PMID: 32755306]
• Winnay JN, Solheim MH, Sakaguchi M et al. Inhibition of the PI 3-kinase pathway disrupts the unfolded protein response and reduces sensitivity to ER stress-dependent apoptosis FASEB J. 2020-08-03 [PMID: 32744782]
• Galeone A, Adams JM, Matsuda S et al. Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation Elife 2020-07-28 [PMID: 32720893]
• Yue XY, Sun XF, Che K et al. Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle Biochem. Biophys. Res. Commun. 2020-08-20 [PMID: 32703455] (WB, Mouse)
• Cicalese S, Okuno K, Elliott KJ et al. 78 kDa Glucose-Regulated Protein Attenuates Protein Aggregation and Monocyte Adhesion Induced by Angiotensin II in Vascular Cells Int J Mol Sci 2020-07-15 [PMID: 32679678] (WB, Rat)
• Hotokezaka Y, Katayama I, Nakamura T ATM-associated signalling triggers the unfolded protein response and cell death in response to stress Commun Biol 2020-07-14 [PMID: 32665601] (WB, Human)
• Murray, H C, Dieriks, B V Et al. The unfolded protein response is activated in the olfactory system in Alzheimer's disease. Acta Neuropathol Commun 2020-07-14 [PMID: 32665027] (IF/IHC, Mouse)
• Zhao Y, Ma S, Cao M, Sun C G604S-HERG mutation in LQT2 leads to autophagy via the UPR-related pathway J. Biosci. 2020-07-15 [PMID: 32661217] (WB, Human)
• Wu XL, Deng MZ, Gao ZJ et al. Neferine alleviates memory and cognitive dysfunction in diabetic mice through modulation of the NLRP3 inflammasome pathway and alleviation of endoplasmic-reticulum stress Int. Immunopharmacol. 2020-05-08 [PMID: 32402951] (WB, Mouse)
• Li C, Deng M, Gao Z et al. Effects of compound K, a metabolite of ginsenosides, on memory and cognitive dysfunction in db/db mice involves inhibition of ER stress and NLRP3 inflammasome pathway Food Funct. 2020-04-13 [PMID: 32374299] (WB)
• Bellini L, Strub T, Habel N et al. Endoplasmic reticulum stress mediates resistance to BCL-2 inhibitor in uveal melanoma cells Cell Death Discov 2020-04-17 [PMID: 32337074]
• Liu D, Zhang P, Zhou J et al. TNFAIP3 Interacting Protein 3 Overexpression Suppresses Nonalcoholic Steatohepatitis by Blocking TAK1 Activation Cell Metab. 2020-04-07 [PMID: 32268115]
• Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules 2020-03-03 [PMID: 32138264] (WB, Human)
• Hinte F, van Anken E, Tirosh B, Brune W Repression of viral gene expression and replication by the unfolded protein response effector XBP1u Elife 2020-02-17 [PMID: 32065579] (WB, Mouse)
• Liu XQ, Deng YX, Dai Z et al. Sodium tanshinone IIA sulfonate protects against A alpha 1-42-induced cellular toxicity by modulating A beta-degrading enzymes in HT22 cells Int. J. Biol. Macromol. 2020-02-06 [PMID: 32035959] (WB, Human)
• Woo M, Choi HI, Park SH et al. The unc-51 like autophagy activating kinase 1-autophagy related 13 complex has distinct functions in tunicamycin-treated cells Biochem. Biophys. Res. Commun. 2020-02-05 [PMID: 32035621]
• Maeyashiki C, Melhem H, Hering L et al. Activation of pH-Sensing Receptor OGR1 (GPR68) Induces ER Stress Via the IRE1 alpha/JNK Pathway in an Intestinal Epithelial Cell Model Sci Rep 2020-01-29 [PMID: 31996710] (WB, Human)
• Han L, Bittner S, Dong D et al. Molecular changes in hepatic metabolism in ZDSD rats-A new polygenic rodent model of obesity, metabolic syndrome, and diabetes Biochim Biophys Acta Mol Basis Dis 2020-01-24 [PMID: 31987840] (WB, Rat)
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• Enos R T, Velazquez K T et al. A Low Dose of Dietary Quercetin Fails to Protect against the Development of an Obese Phenotype in Mice. PLoS One 2016-12-14 [PMID: 27959936] (WB, Mouse)
• Mukhopadhyay C, Triplett A et al. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. Proc Natl Acad Sci U S A 2016-12-20 [PMID: 27930322] (WB, Mouse)
• Takatori O, Usui S, Okajima M et al. Sodium 4-Phenylbutyrate Attenuates Myocardial Reperfusion Injury by Reducing the Unfolded Protein Response. J. Cardiovasc. Pharmacol. Ther. 2016-12-01 [PMID: 27909014] (WB, Mouse)
• Liu X, Zhao J, Liu Q et al. MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3. Sci Rep. 2016-11-15 [PMID: 27845424] (WB, Human)
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• Cicalese S Exploring the Role of Smooth Muscle GRP78 in Angiotensin II-Induced Vascular Amyloid Deposition and Remodeling Thesis 2022-01-01
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  Details:
  p-IRE1 (Ser724) antibody used for Immunofluorescence staining on human PMBCs that were untreated or treated with intralipid for 5 hours (Figure 4C and D).
• Lopez-Gonzalez I, Perez-Mediavilla A, Zamarbide M et al. Limited Unfolded Protein Response and Inflammation in Neuroserpinopathy. J Neuropathol Exp Neurol. [PMID: 26733586] (WB, Human)
• Vannuvel K, Van Steenbrugge M, Demazy C et al. Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population. J. Cell. Physiol. 2015-12-17 [PMID: 26680008] (WB, Human)
• El-Khattouti A, Selimovic D, Hannig M et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-kB inhibition. J Cell Mol Med. [PMID: 26578344] (WB, Human)
• Kim Ok-Kyung, Nam Da-Eun, Jun Woojin, Lee Jeongmin. Cudrania tricuspidata water extract improved obesity-induced hepatic insulin resistance in db/db mice by suppressing ER stress and inflammation. Food Nutr Res 2015-01-01 [PMID: 26507490] (WB, Mouse)
• Jensen BK, Monnerie H, Mannell MV et al. Altered Oligodendrocyte Maturation and Myelin Maintenance: The Role of Antiretrovirals in HIV-Associated Neurocognitive Disorders. J Neuropathol Exp Neurol. [PMID: 26469251] (WB, Mouse)
• Aumailley L, Garand C, Dubois MJ et al. Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice. PLoS One 2015-01-01 [PMID: 26447695] (ICC/IF, Mouse)
• Concha NO, Smallwood A, Bonnette W et al. Long-Range Inhibitor-Induced Conformational Regulation of Human IRE1alpha Endoribonuclease Activity. Mol Pharmacol. [PMID: 26438213] (WB, Human)
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• Cai X, Chen L, McGinnis JF. Correlation of ER stress and retinal degeneration in tubby mice. Exp. Eye Res. 2015-09-08 [PMID: 26325328] (WB, Mouse)
• Wang Y, Yamada E, Zong H, Pessin JE. Fyn Activation of mTORC1 Stimulate the IRE1a-JNK Pathway Leading to Cell Death. J. Biol. Chem. 2015-08-25 [PMID: 26306048] (WB, Mouse, Human)
• Naughton MC, McMahon JM, FitzGerald U. Differential activation of ER stress pathways in myelinating cerebellar tracts. Int. J. Dev. Neurosci. 2015-08-20 [PMID: 26297908] (IHC-P, Rat)
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• Quan X, Wang J, Liang C et al. Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells Biochem. Biophys. Res. Commun. 2015-06-12 [PMID: 26079879] (WB, Mouse)
  
  Details:
  IRE1 alpha antibody was used for WB on the lysates of C2C12 cells ( a primary line of murine myoblasts) that were serum-starved and treated with 0.5 ug/mL tunicamycin or without in the presence or absence of 100 nM melatonin or 10 uM Luzindole for 16 hours (Fig. 3).
• Bi X, He X, Xu M et al. Acetylcholine ameliorates endoplasmic reticulum stress in endothelial cells after hypoxia/reoxygenation via M3 AChR-AMPK signaling Cell Cycle. 2015-06-11 [PMID: 26066647] (WB, Human)
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• Borsa M, Ferreira PL, Petry A et al. HIV infection and antiretroviral therapy lead to unfolded protein response activation. Virol J. [PMID: 25976933] (WB, Human)
• Won Suk Jahng J, Turdi S, Kovacevic V et al. Pressure overload-induced cardiac dysfunction in aged male adiponectin knockout mice is associated with autophagy deficiency. Endocrinology. 2015-05-11 [PMID: 25961840] (WB, Mouse)
  
  Details:
  IRE1 alpha [p Ser724] antibody used for WB analysis of mouse left ventricular (LV) heart tissue after 3 weeks of pressure overload (PO) induction (Fig 6).
• Shen Y, Yang J, Zhao J et al. The switch from ER stress-induced apoptosis to autophagy via ROS-mediated JNK/p62 signals: A survival mechanism in methotrexate-resistant choriocarcinoma cells Exp. Cell Res. 2015-04-24 [PMID: 25912909] (WB, Human)
• Hotokezaka Y, Katayama I, van Leyen K, Nakamura, T. GSK-3beta-dependent downregulation of gamma-taxilin and alphaNAC merge to regulate ER stress responses Cell Death Dis 2015-04-17 [PMID: 25880086] (WB, Mouse)
• Qiu B, Ackerman D, Sanchez DJ et al. HIF-2alpha dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma Cancer Discov 2015-03-31 [PMID: 25829424] (WB, Human)
• Narsale AA, Enos RT, Puppa MJ et al. Liver Inflammation and Metabolic Signaling in ApcMin/+ Mice: The Role of Cachexia Progression. PLoS ONE. 2015-03-20 [PMID: 25789991] (WB, Mouse)
• Ricci D, Tutton S, Marrocco I et al. The connector between the kinase and RNase domains of IRE1alpha transmits the conformational change that underlies ER stress-induced activation. bioRxiv 2020-01-01 (WB, Human)
• Jeon YJ, Khelifa S, Ratnikov B et al. Regulation of Glutamine Carrier Proteins by RNF5 Determines Breast Cancer Response to ER Stress-Inducing Chemotherapies. Cancer Cell. 2015-03-09 [PMID: 25759021] (WB, Human)
• Iwasaki N, Sugiyama Y, Miyazaki S et al. An ATF4-Signal-Modulating Machine Other Than GADD34 Acts in ATF4-to-CHOP Signaling to Block CHOP Expression in ER-Stress-Related Autophagy J. Cell. Biochem. 2015-03-03 [PMID: 25737469] (WB, Human)
• Wu H, Wei L, Fan F et al. Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. Nat Commun. [PMID: 25695629]
• Jaishy B, Zhang Q, Chung HS et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. [PMID: 25529920] (WB, Mouse)
• Kim DI, Park MJ, Choi JH et al. Hyperglycemia-induced GLP-1R downregulation causes RPE cell apoptosis. Int J Biochem Cell Biol. [PMID: 25483438] (WB, Human)
• Li E, Xu Z, Liu F et al. Continual exposure to cigarette smoke extracts induces tumor-like transformation of human nontumor bronchial epithelial cells in a microfluidic chip. J Thorac Oncol. [PMID: 25157762] (WB, Human)
• Kosakowska-Cholody T, Lin J, Srideshikan S M et al. HKH40A downregulates GRP78/BiP expression in cancer cells. Cell Death Dis. 2014-05-22 [PMID: 24853418] (WB)
• Shao Mengle, Shan Bo, Liu Yang et al. Hepatic IRE1alpha regulates fasting-induced metabolic adaptive programs through the XBP1s-PPARalpha axis signalling. Nat Commun. 2014-03-27 [PMID: 24670948] (WB)
• Zhao M, Zang B, Cheng M et al. Differential responses of hepatic endoplasmic reticulum stress and inflammation in diet-induced obese rats with high-fat diet rich in lard oil or soybean oil. PLoS One. [PMID: 24223162] (WB, Rat)
• Wang YI, Bettaieb A, Sun C et al. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress. PLoS One. [PMID: 24205197] (WB, Human)
• Adolph TE, Tomczak MF, Niederreiter L et al. Paneth cells as a site of origin for intestinal inflammation. Nature. [PMID: 24089213]
• Xu B, Shan M, Wang F et al. Endoplasmic reticulum stress signaling involvement in manganese-induced nerve cell damage in organotypic brain slice cultures. Toxicol Lett. [PMID: 23948121] (WB, Rat)
• Lind KR, Ball KK, Cruz NF, Dienel GA. The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes. Neurochem Int. [PMID: 23411409] (WB, Rat)
• Han J, Murthy R, Wood B et al. ER stress signalling through eIF2alpha and CHOP, but not IRE1alpha, attenuates adipogenesis in mice Diabetologia 2013-01-12 [PMID: 23314846] (WB, Mouse)
• Otoda T, Takamura T, Misu H et al. Proteasome Dysfunction Mediates Obesity-Induced Endoplasmic Reticulum Stress and Insulin Resistance in the Liver Diabetes 2012-12-03 [PMID: 23209186] (WB, Mouse, Rat)
• Cha SI, Ryerson CJ, Lee JS et al. Cleaved cytokeratin-18 is a mechanistically informative biomarker in idiopathic pulmonary fibrosis Respir Res 2012-11-20 [PMID: 23167970] (WB, Human)
• Bartolome A, Guillen C, Benito M et al. Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic beta cell death. Autophagy 2012-12-01 [PMID: 22951927]
• Ri M, Tashiro E, Oikawa D et al. Identification of Toyocamycin, an agent cytotoxic for multiple myeloma cells, as a potent inhibitor of ER stress-induced XBP1 mRNA splicing Blood Cancer J 2012-07-01 [PMID: 22852048] (WB, Human)
• Kawabata S, Gills JJ, Mercado-Matos JR et al. Synergistic effects of nelfinavir and bortezomib on proteotoxic death of NSCLC and multiple myeloma cells. Cell Death Dis. [PMID: 22825471] (WB, Human)
• Dickie LJ, Aziz AM, Savic S et al. Involvement of X-box binding protein 1 and reactive oxygen species pathways in the pathogenesis of tumour necrosis factor receptor-associated periodic syndrome. Ann Rheum Dis. [PMID: 22679299]
• Jheng JR, Lin CY, Horng JT, Lau KS. Inhibition of enterovirus 71 entry by transcription factor XBP1. Biochem Biophys Res Commun. [PMID: 22469468] (WB, Human)
• Ota A, Wang Y. Cdc37/Hsp90-mediated regulation of IRE1[alpha] activity in ER stress response and insulin synthesis in INS-1 cells. The Journal of Biological Chemistry. 2011-12-23 [PMID: 22199355] (WB, Human)
• Kimura K, Yamada T, Matsumoto M et al. Endoplasmic reticulum stress inhibits STAT3-dependent suppression of hepatic gluconeogenesis via dephosphorylation and deacetylation Diabetes 2012-01-01 [PMID: 22124464] (WB, Mouse)
• Nijholt DA, van Haastert ES, Rozemuller AJ, Scheper W, Hoozemans JJ. The unfolded protein response is associated with early tau pathology in the hippocampus of tauopathies. J Pathol doi: 10.1002/path.3969. 2011-11-21 [PMID: 22102449] (IHC-P, Human)
• Michallet A-S, Mondiere P, Taillardet M et al. Compromising the unfolded protein response induces autophagy-mediated cell death in multiple myeloma cells. PLoS ONE;6(10). 2011-01-01 [PMID: 22028791] (WB, Human)
• Hage Hassan R, Hainault I, Vilquin J-T et al. Endoplasmic reticulum stress does not mediate palmitate-induced insulin resistance in mouse and human muscle cells. Diabetologia. 2011-10-18 [PMID: 22006247] (WB, Mouse)
• Moumen A, Virard I, Raoul C. Accumulation of wildtype and ALS-linked mutated VAPB impairs activity of the proteasome. PLoS One. 1905-07-03 [PMID: 21998752] (WB, Primate)
• Shimada Y, Kobayashi H, Kawagoe S et al. Endoplasmic reticulum stress induces autophagy through activation of p38 MAPK in fibroblasts from Pompe disease patients carrying c. 546G>T mutation. Molecular Genetics and Metabolism. 2011-09-14 [PMID: 21982629] (WB, Human)
• Merquiol E, Uzi D, Mueller T et al. HCV causes chronic endoplasmic reticulum stress leading to adaptation and interference with the unfolded protein response. PLoS One. 1905-07-03 [PMID: 21949742] (WB, Human)
• Castillo K, Rojas-Rivera D, Lisbona F et al. BAX inhibitor-1 regulates autophagy by controlling the IRE1? branch of the unfolded protein response. The EMBO Journal. 2011-09-16 [PMID: 21926971] (WB, Mouse)
• Chiang Chih-Kang, Hsu Shih-Ping, Wu Cheng-Tien et al. Endoplasmic reticulum stress implicated in the development of renal fibrosis. Mol Med. 2011-01-01 [PMID: 21863214] (WB, Rat)
• Chen K, Jin P, He HH et al. Overexpression of Insig-1 protects beta cell against glucolipotoxicity via SREBP-1c. J Biomed Sci 2011-01-01 [PMID: 21843373] (WB, Rat)
• Wang FM, Galson DL, Roodman GD, Ouyang H. Resveratrol triggers the pro-apoptotic endoplasmic reticulum stress response and represses pro-survival XBP1 signaling in human multiple myeloma cells. Exp Hematol. [PMID: 21723843] (WB, Human)
• Oslowski CM, Urano F. Measuring ER stress and the unfolded protein response using mammalian tissue culture system. Methods Enzymol 2011-01-01 [PMID: 21266244] (Human)
• Zheng Z, Zhang C, Zhang K. Measurement of ER stress response and inflammation in the mouse model of nonalcoholic fatty liver disease. Methods Enzymol. 1905-07-03 [PMID: 21266239] (WB, Mouse)
• Makioka K, Yamazaki T, Fujita Y et al. Involvement of endoplasmic reticulum stress defined by activated unfolded protein response in multiple system atrophy. J Neurol Sci 2010-10-15 [PMID: 20667553] (IF/IHC, Human)
• Langou K, Moumen A, Pellegrino C et al. AAV-mediated expression of wild-type and ALS-linked mutant VAPB selectively triggers death of motoneurons through a Ca2+-dependent ER-associated pathway. J Neurochem. [PMID: 20477942] (WB, ICC/IF, Mouse)
• Winnay JN, Boucher J, Mori MA et al. A regulatory subunit of phosphoinositide 3-kinase increases the nuclear accumulation of X-box-binding protein-1 to modulate the unfolded protein response. Nat Med 2010-04-01 [PMID: 20348923] (Mouse)
• Qiu Y, Mao T, Zhang Y et al. A crucial role for RACK1 in the regulation of glucose-stimulated IRE1alpha activation in pancreatic beta cells. Sci Signal;3(106):ra7. 2010-01-26 [PMID: 20103773] (WB, Human)
• Cretenet G, Le Clech M, Gachon F. Circadian clock-coordinated 12 Hr period rhythmic activation of the IRE1alpha pathway controls lipid metabolism in mouse liver. Cell Metab. [PMID: 20074527] (WB, Mouse)
• liu x, Huang S, Wan C et al. Melatonin Attenuates Scopolamine-induced Cognitive Dysfunction through SIRT1/IRE1?/XBP1 pathway Research Square 2023-04-20 (WB, IHC, Mouse)
• Walter F, Schmid J, Dussmann H et al. Imaging of single cell responses to ER stress indicates that the relative dynamics of IRE1/XBP1 and PERK/ATF4 signalling rather than a switch between signalling branches determine cell survival Cell Death Differ. 2015-01-30 [PMID: 25633195] (WB, Human)
• Clarke JR, Lyra E Silva NM, Figueiredo CP et al. Alzheimer-associated Abeta oligomers impact the central nervous system to induce peripheral metabolic deregulation. EMBO Mol Med 2015-02-01 [PMID: 25617315] (WB)
• Ishimura S, Furuhashi M, Mita T et al. Reduction of endoplasmic reticulum stress inhibits neointima formation after vascular injury Sci Rep et al. 2014-11-06 [PMID: 25373918] (IHC-P, WB, Mouse)
  
  Details:
  IRE1 alpha [p Ser724] antibody used for WB and IHC-P on experiments involving mouse's wire injury model (endoluminal injury of the left femoral artery). IHC-P - femoral arteries fixed in 10% formalin, sectioned at ten locations at 100-um intervals, On 4C primary antibody incubation (Figure 1D). WB - Antibody used as ER stress marker in Xbp1-knockdown human coronary artery smooth muscle cells (CASMC) treated or not with PDGF-BB, 4-PBA and TUDCA under various in-vitro treatment conditions (Figure 2H, Figure 4A, Figure 4B, Figure S4, )
• Yang Y, Sun M, Shan Y et al. Endoplasmic Reticulum Stress-Mediated Apoptotic Pathway Is Involved in Corpus Luteum Regression in Rats. Reprod Sci. 2014-10-20 [PMID: 25332219] (IHC-P, Mouse)
• Wu Y, Yang M, Fan J et al. Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis. Cell Death Dis. 2014-10-17 [PMID: 25321471] (WB, Mouse)
• Park KS, Bayles I, Szlachta-McGinn A et al. Transcription Elongation Factor ELL2 Drives Ig Secretory-Specific mRNA Production and the Unfolded Protein Response. J Immunol. 2014-09-19 [PMID: 25238757]
• Zhu X, Zhang J, Sun H et al. Ubiquitination of inositol-requiring enzyme 1 (IRE1) by the E3 ligase CHIP mediates the IRE1/TRAF2/JNK pathway. J Biol Chem. 2014-09-15 [PMID: 25225294]
• Kurahashi T, Kwon M, Homma T et al. Reductive detoxification of acrolein as a potential role for aldehyde reductase (AKR1A) in mammals. Biochem. Biophys. Res. Commun. 2014-08-21 [PMID: 25152401] (WB, Mouse)
• Kohler C, Dinekov M, Gotz J. Granulovacuolar degeneration and unfolded protein response in mouse models of tauopathy and Ab amyloidosis. Neurobiol. Dis. 2014-07-27 [PMID: 25073087] (IHC-P, Mouse)
  
  Details:
  IRE1 alpha (phospho-Ser 724) antibody used for IHC-P in transgenic pR5 mice (which express the longest human tau isoform, htau40, together with the pathogenic mutation P301L). Brains fixed in 4%PFA, antigen retrieval performed by boiling the sections 3 times for 5 min in citrate buffer pH 6.0 using microwave, primary dilution used - 1:3500, detection by avidin-biotin peroxidase - DAB/0.6% ammonium nickel sulfate - deep blue-gray reaction product (Fig 1d).
• Negroni A, Prete E, Vitali R et al. Endoplasmic reticulum stress and unfolded protein response are involved in paediatric inflammatory bowel disease Dig Liver Dis. 2014-06-19 [PMID: 24953208] (WB, Human)
  
  Details:
  phospho Ser724 - IRE1 alpha antibody used for WB (1:500 dilution, ON 4C incubation in 3% milk) on 20ug lysates of mucosal biopsy specimens collected from ileal and colonic district of inflammatory bowel disease paediatric patients and controls. See full text for details on tissue lysate preparation and WB analysis. Fig. 4 : WB image shows an increased pIRE expression in tissue lysates of inflamed colon of ulcerative colitis (UC) and Crohn's disease (CD) patients when compared to control subjects (Ctrl).
• Liew CW, Assmann A, Templin AT et al. Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic beta cells. Proc. Natl. Acad. Sci. U.S.A. 2014-05-19 [PMID: 24843127] (WB, Mouse)
• Bonnet Wersinger D, Benkafadar N, Jagodzinska J et al. Impairment of Visual Function and Retinal ER Stress Activation in Wfs1-Deficient Mice. PLoS ONE. 2014-05-14 [PMID: 24823368] (IHC-Fr, Mouse)
• Cai XH, Li XC, Jin SW et al. Endoplasmic reticulum stress plays critical role in brain damage after chronic intermittent hypoxia in growing rats. Exp. Neurol. 2014-05-05 [PMID: 24810321] (WB, Rat)
• Hosoi T, Inoue Y, Nakatsu K et al. TERT attenuated ER stress-induced cell death. Biochem. Biophys. Res. Commun. 2014-04-16 [PMID: 24746472] (WB, Human)
• Koyama M, Furuhashi M et al. Reduction of endoplasmic reticulum stress by 4-phenylbutyric acid prevents the development of hypoxia-induced pulmonary arterial hypertension. Am J Physiol Heart Circ Physiol 2014-01-05 [PMID: 24610918] (WB, Human)
• Suh HN, Lee YJ, Kim MO, Han HJ. Glucosamine-Induced Sp1 O-GlcNAcylation Ameliorates Hypoxia-Induced SGLT Dysfunction in Primary Cultured Renal Proximal Tubule Cells. J. Cell. Physiol. 2014-03-04 [PMID: 24591095] (WB, Rabbit)
• Lu G, Ota A, Ren S et al. PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response. Mol Metab 2013-01-01 [PMID: 24327956]
• Liu L, Fan Z, Tang Y, Ke Z. The Resveratrol Attenuates Ethanol-Induced Hepatocyte Apoptosis Via Inhibiting ER-Related Caspase-12 Activation and PDE Activity In Vitro. Alcohol Clin Exp Res. 2013-11-13 [PMID: 24224909] (WB, Human)
• Matsumoto H, Miyazaki S, Matsuyama S et al. Selection of autophagy or apoptosis in cells exposed to ER-stress depends on ATF4 expression pattern with or without CHOP expression. Biol Open. 2013-08-27 [PMID: 24167719] (WB, Human)
• Jung DY, Chalasani U, Pan N et al. KLF15 Is a Molecular Link between Endoplasmic Reticulum Stress and Insulin Resistance. PLoS One. 2013-10-22 [PMID: 24167585] (WB, Mouse)
• Omikorede O, Qi C, Gorman T et al. ER stress in rodent islets of Langerhans is concomitant with obesity and beta-cell compensation but not with beta-cell dysfunction and diabetes. Nutr Diabetes. 2013-10-21 [PMID: 24145577] (WB, Mouse)
• Lim SK, Choi H, Park MJ et al. The ER stress-mediated decrease in DDAH1 expression is involved in formaldehyde-induced apoptosis in lung epithelial cells. Food Chem Toxicol. 2013-10-16 [PMID: 24140967] (WB, Human)
• Yang C, Diiorio P, Jurczyk A et al. Pathological endoplasmic reticulum stress mediated by the IRE1 pathway contributes to pre-insulitic beta cell apoptosis in a virus-induced rat model of type 1 diabetes. Diabetologia 2013-12-01 [PMID: 24121653] (WB, Rat)
• Kemp KL, Lin Z, Zhao F et al. The Serine-threonine Kinase Inositol-requiring Enzyme 1alpha (IRE1alpha) Promotes IL-4 Production in T Helper Cells. J Biol Chem. 2013-11-15 [PMID: 24100031] (WB, Mouse)
• Chen YC, Zeng XY, He Y et al. Rutaecarpine Analogues Reduce Lipid Accumulation in Adipocytes via Inhibiting Adipogenesis/Lipogenesis with AMPK Activation and UPR Suppression. ACS Chem Biol. 2013-10-18 [PMID: 23962138] (WB, Mouse)
• Kujiraoka T, Satoh Y, Ayaori M et al. Hepatic extracellular signal-regulated kinase 2 suppresses endoplasmic reticulum stress and protects from oxidative stress and endothelial dysfunction. J Am Heart Assoc. 2013-08-16 [PMID: 23954796] (WB, Mouse)
• Stahl S, Burkhart JM, Hinte F et al. Cytomegalovirus Downregulates IRE1 to Repress the Unfolded Protein Response. PLoS Pathog. 2013-08-01 [PMID: 23950715] (WB, Human)
• Kitai Y, Ariyama H, Kono N et al. Membrane lipid saturation activates IRE1alpha without inducing clustering. Genes Cells 2013-06-27 [PMID: 23803178] (WB, Human)
• Song Y, Shen H, Du W, Goldstein DR. Inhibition of x-box binding protein 1 reduces tunicamycin-induced apoptosis in aged murine macrophages. Aging Cell 2013-05-28 [PMID: 23711292] (WB, Mouse)
• Koh HJ, Toyoda T, Didesch MM et al. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat Commun 2013-01-01 [PMID: 23695665] (WB, Mouse)
• Akiyama M, Liew CW, Lu S et al. X-box binding protein 1 is essential for insulin regulation of pancreatic alpha cell function Diabetes 2013-03-14 [PMID: 23493568]
• Zheng M, Zhang Q, Joe Y et al. Curcumin induces apoptotic cell death of activated human CD4+ T cells via increasing endoplasmic reticulum stress and mitochondrial dysfunction. Int Immunopharmacol 2013-02-15 [PMID: 23415873] (WB, Human)
• Arshad M, Ye Z, Gu X et al. RNF13, a RING Finger Protein, Mediates Endoplasmic Reticulum Stress-induced Apoptosis through the Inositol-requiring Enzyme (IRE1alpha)/c-Jun NH2-terminal Kinase Pathway. J Biol Chem 2013-03-22 [PMID: 23378536] (WB, Human)
• Toosi S, Orlow SJ, Manga P. Vitiligo-Inducing Phenols Activate the Unfolded Protein Response in Melanocytes Resulting in Upregulation of IL6 and IL8 J Invest Dermatol 2012-06-14 [PMID: 22696056] (WB, Human)
• Zheng X, Zheng X, Wang X et al. Acute hypoxia induces apoptosis of pancreatic B-cell by activation of the unfolded protein response and upregulation of CHOP. Cell Death Dis. 2012-06-14 [PMID: 22695615] (WB, Mouse)
• Zheng M, Kim SK, Joe Y, Back SH, Cho HR, Kim HP, Ignarro LJ, Chung HT. Sensing endoplasmic reticulum stress by protein kinase RNA-like endoplasmic reticulum kinase promotes adaptive mitochondrial DNA biogenesis and cell survival via heme oxygenase-1/carbon monoxide activity. FASEB J;26(6):2558-68. 2012-06-01 [PMID: 22391129] (WB, Mouse)
• Wu LL, Russell DL, Norman RJ, Robker RL. Endoplasmic Reticulum (ER) Stress in Cumulus-Oocyte Complexes Impairs Pentraxin-3 Secretion, Mitochondrial Membrane Potential ({Delta}{Psi}m), and Embryo Development. Mol Endocrinol;26(4):562-73. 2012-04-01 [PMID: 22383462] (WB, Mouse)
• Morcavallo A, Genua M, Palummo A et al. Insulin and insulin-like growth factor II differentially regulate endocytic sorting and stability of the insulin receptor isoform A J Biol Chem 2012-02-08 [PMID: 22318726] (ELISA, Mouse)
• Teodoro T, Odisho T, Sidorova E, Volchuk A. Pancreatic beta-cells depend on basal expression of active ATF6?-p50 for cell survival even under nonstress conditions. Am J Physiol Cell Physiol;302(7):C992-C1003. 2012-04-01 [PMID: 22189555] (WB, Human)
• Yamagishi N, Ueda T, Mori A et al. Decreased expression of endoplasmic reticulum chaperone GRP78 in liver of diabetic mice. Biochemical and Biophysical Research Communications. 2011-12-01 [PMID: 22155243] (Mouse)
• Wang C-T, Jetzt AE, Cheng J-S, Cohick WS. Inhibition of the unfolded protein response by ricin a-chain enhances its cytotoxicity in Mammalian cells. Toxins (Basel)3(5):453-68. 2011-05-01 [PMID: 22069719] (WB, Human)
• Chuang T-C, Hsu S-C, Cheng Y-T et al. Magnolol down-regulates HER2 gene expression, leading to inhibition of HER2-mediated metastatic potential in ovarian cancer cells. Cancer Lett;311(1):11-9. 2011-12-01 [PMID: 21757288]
• Lim MP, Devi LA, Rozenfeld R. Cannabidiol causes activated hepatic stellate cell death through a mechanism of endoplasmic reticulum stress-induced apoptosis. Cell Death Dis;2:e170. 2011-06-09 [PMID: 21654828] (WB, Rat, Mouse)
• Wang N, Zhang D, Mao X et al. Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes. Mol Cell Endocrinol. [PMID: 19524131] (WB, Rat)
• Kashiwakura Y, Ochiai K, Watanabe M et al. Down-regulation of Inhibition of Differentiation-1 via Activation of Activating Transcription Factor 3 Smad Regulates REIC/Dickkopf-3-Induced Apoptosis. Cancer Res;68(20):8333-8341. 2008-01-01 [PMID: 18922905] (WB, Human)
• Sugeno N, Takeda A, Hasegawa T et al. Serine 129 Phosphorylation of {alpha}-Synuclein Induces Unfolded Protein Response-mediated Cell Death. J Biol Chem;283(34):23179-23188. 2008-01-01 [PMID: 18562315] (WB, Human)
• Lipson, KL et al. Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IER1. Cell Metabolism 4: 245-254. 2006-01-01 [PMID: 16950141] (WB, IP, Mouse, Rat, Human)
• Imbernon Piedra M. Efectos centrales de MCH sobre el metabolismo periferico. Thesis 2016-06-01 (IF/IHC, WB)
• Kim J, Youn I, Seo E, Kim C Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice Research Square 2023-06-27 (WB, Mouse)
• Hong M. Regulation of Human Pancreatic B-cell Proliferation by Localization, Interaction, and Transcription of Endogenously Expressed Cell Cycle Molecules Thesis 2017-01-01 (Human)
• Hoozemans JJ, van Haastert ES, Nijholt DA et al. The Unfolded Protein Response Is Activated in Pretangle Neurons in Alzheimer';s Disease Hippocampus. Am J Pathol. 2009-03-05 [PMID: 19264902] (IHC-P, Human)
• Luo D, He Y, Zhang H et al. AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response. J Biol Chem;283(18):11905-12. 2008-05-02 [PMID: 18281285] (WB, IP, Mouse)

Reviews for IRE1 alpha Antibody (NB100-2323) (9)

Average Rating: 4.4

(Based on 9 reviews)

We have 9 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Takujiro Homma WB Human 03/19/2021

Summary

• Application: Western Blot
• Sample Tested: hela cell
• Species: Human
• Lot: AB-1

Comments

• Comments: Reduced Denaturing (8% gel)
  Milk as blocking agent for 1 h; Concentration: 5 %; Temperature: 25°C
  1st Ab Incubation time: 16 h; Temperature: 4°C; Diluent: 1% skim milk in TBST

reviewed by: Verified Customer WB Rat 10/05/2020

Summary

• Application: Western Blot
• Sample Tested: Placental tissue
• Species: Rat
• Lot: AW

Comments

• Comments: Rat placenta lysates, reduced and denatured. 100 ug total protein resolved on 7.5% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Block with 50% Rockland blocking solution. Incubate membrane overnight at 4C with 1:500 dilution of pIRE1a antibody. Washes with PBS-0.1% tween 20. Incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-rabbit IgG. Images taken with Odyssey V3.0.

reviewed by: Zeyuan Zhang WB Mouse 09/25/2020

Summary

• Application: Western Blot
• Sample Tested: Liver
• Species: Mouse
• Lot: AM

reviewed by: Jennifer Fox WB Mouse 10/22/2019

Summary

• Application: Western Blot
• Sample Tested: macrophage whole cell lysate
• Species: Mouse
• Lot: AW

Comments

• Comments: 1:1000 dilution

reviewed by: Shinobu Miyazaki ICC Mouse 11/19/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse vascular smooth muscle cell
• Species: Mouse
• Lot: AI-1

Comments

• Comments: 0.1 µM Thapsigargin for 2 hours
  Fixation: 4%PFA/PBS for 10 minutes RT
  Permeabilization: 50 µg/ml digitonin PBS for 10 minutes RT,
  Blocking: 0.1% gelatin/PBS for 30minutes RT
  primary antibody for 1 hour RT
  secondary anti rabbit IgG Alexa 488 1:200 against p-IRE1 (ser724) for 1 hour RT

reviewed by: Verified Customer WB Mouse 04/03/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: AO-1

Comments

• Comments: C57BL/6 Mice

reviewed by: Verified Customer WB Mouse 03/15/2017

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates: Hamster, Mouse, Human, Monkey
• Species: Mouse
• Lot: AO-1

Comments

• Comments: 6132 Pro cells (mouse fibrosarcoma) were treated with or without 10 mM DTT for 3 hr and total lysates were prepared. The membrane blot was incubated with 5 ul of primary antibody (IRE1 alpha [p Ser724]) in 10 ml of blocker (2% BSA in TBS containing 0.05% Tween20) for 2hr at RT, followed by incubation with HRP-conjugated secondary antibody (anti-rabbit IgG H+L, from Pierce) for 1hr. The signal was visualized using chemilluminedcent substrate (Pico, from Pierce).

reviewed by: cynthia morimoto WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 22951927
• Species: Mouse

reviewed by: System Admin WB Mouse 05/27/2009

Summary

• Application: Western Blot
• Sample Tested: Mouse lens tissue, Sample Amount: 40ug
• Species: Mouse
• Lot: I

FAQs for IRE1 alpha Antibody (NB100-2323). (Showing 1 - 4 of 4 FAQs).

1. Can you please tell me the immunogen sequence of this antibody?
   • The immunogen of NB100-2323 is a synthetic peptide from an amino acids sequence surrounding the phosphorylated serine 724 of the human IRE1 alpha protein (Swiss-Prot #O75460). Unfortunately, I cannot provide any additional detail, as that is proprietary information.
2. What is the size of the western blot band?
   • The weight observed in our in-house testing is typically 100-110kDa
3. Can this antibody detect non-phosphorylated IRE1a?
   • NB100-2323 is phospho-specific. In all of our testing done with this antibody, we have only detected the phosphorylated IRE1a protein. There has not been any observed cross reactivity with the non-phosphorylated protein.
4. I wish to use this antibody for cultured cardiomyocytes /Immunofluorescence. I am wondering if this antibody works for Immunocytochemistry/Immunofluorescence for cultured cells? Specifically, we wish to compare IRE1 phosphorylation under stress condition to non-stress condition. Because the nature of IRE1 phosphorylation is part of stress response pathway. I am wondering if the immunofluorescence with your antibody is sensitive enough to distinguish p-IRE1 signal under stress condition to the endogenous stress (no stress inducer treatment) in the cell?
   • IRE1 p Ser724 antibody (NB100-2323) antibody has been validated in Immunocytochemistry/Immunofluorescence (ICC/IF) and we guarantee it 100% for the applications and species listed on our datasheet. As long as your cultured cardiomyocytes express the protein target that is phosphorylated at Ser 724 amino acid residue, our antibody will detect it.  You may learn more about our guaranteee policy here

Bioinformatics

• Gene Symbol: ERN1