PADI1 Antibody (8M9P3)

Western Blot: PADI1 Antibody (8M9P3) [NBP3-33463] - Western blot analysis of lysates from HaCaT cells using PADI1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Western Blot: PADI1 Antibody (8M9P3) [NBP3-33463] - Western blot analysis of lysates from Mouse ovary using PADI1 Rabbit mAb at 1:1400 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 60 s.

Western Blot: PADI1 Antibody (8M9P3) [NBP3-33463] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PADI1 using PADI1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1 s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 8M9P3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PADI1 Antibody (8M9P3) Summary

• Description: Novus Biologicals Rabbit PADI1 Antibody (8M9P3) (NBP3-33463) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human PADI1.2).
  
  Sequence:
  MAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEVFMVYNRTRVKEPIGKARWPLDTDADMVVSVGTASKELKDFKVRVSYFGEQEDQALGRSVLYLTGVDISLEVDTGRTGKVKRSQGDKKTWRWGPEGYGAILLVNCDRDNHRSAEPDLTHSWLMSLADLQDMSPMLLSCNGPDKLFDSHKLVLNVPFSDSKRVRVFCARGGNSLSDYKQVLGPQCLSYEVERQPGEQEIKFYVEGLTFPDADFLGLVSLSVSLVDPGTL
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PADI1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:1000 - 1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for PADI1 Antibody (8M9P3)

• EC 3.5.3.15
• HPAD10
• hPAD-colony 10
• PAD1
• PDI
• PDI1Protein-arginine deiminase type I
• peptidyl arginine deiminase, type I
• Peptidylarginine deiminase I
• protein-arginine deiminase type-1

Background

Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI1