PERK Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3999) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 130 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PERK knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PERK Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PERK
  Ala29-Gln230
  Accession # Q9NZJ5
• Specificity: Detects human PERK in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EIF2AK3

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Knockout Validated
• Control:

  PERK Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PERK Antibody [Unconjugated]

• DKFZp781H1925
• EC 2.7.11.1
• EIF2AK3
• eukaryotic translation initiation factor 2 alpha kinase 3
• eukaryotic translation initiation factor 2-alpha kinase 3
• hsPEK
• PEK
• PEKPERKPancreatic eIF2-alpha kinase
• PERK
• PRKR-like endoplasmic reticulum kinase
• WRS

Background

PERK, a type 1 ER membrane kinase, mediates eIF2 alpha phosphorylation at Ser51 during the UPR (unfolded protein response). Protein synthesis is inhibited, thereby reducing the burden of protein substrate for the ER folding and degradation mechanism. Phosphorylation of eIF2 alpha also selectively promotes the expression of UPR target genes such as Chop and BiP. PERK may also play a role in tumor cell adaptation to hypoxic stress by regulating the translation of angiogenic factors necessary for the development of functional microvessels. Mutations in PERK are responsible for the rare autosomal-recessive disorder, WRS (Wolcott-Rallison syndrome).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PERK Antibody (AF3999)(3)

Publications using AF3999

• Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. PLoS ONE, 2015;10(3):e0119738. 2015 [PMID: 25780921] (Western Blot, Mouse)
• Atkins C, Liu Q, Minthorn E, Zhang S, Figueroa D, Moss K, Stanley T, Sanders B, Goetz A, Gaul N, Choudhry A, Alsaid H, Jucker B, Axten J, Kumar R Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity. Cancer Res, 2013;73(6):1993-2002. 2013 [PMID: 23333938] (Western Blot, Human)
• Liang G, Yang J, Wang Z Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha. Hum. Mol. Genet., 2008;17(20):3254-62. 2008 [PMID: 18664456] (Western Blot, Human)

Bioinformatics

• Gene Symbol: EIF2AK3
• Uniprot:
  • Q9NZJ5()