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PERK Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity-purified ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PERK knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PERK ...read more
Activation of the ER stress signaling pathway leads to the LX-2 cell apoptosis induced by VP-16.(a) Western blotting analysis of ER stress-associated proteins in LX-2 cells after treatment with VP-16 for 72 h. (b) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

PERK Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PERK
Ala29-Gln230
Accession # Q9NZJ5
Specificity
Detects human PERK in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EIF2AK3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3999 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PERK Antibody [Unconjugated]

  • DKFZp781H1925
  • EC 2.7.11.1
  • EIF2AK3
  • eukaryotic translation initiation factor 2 alpha kinase 3
  • eukaryotic translation initiation factor 2-alpha kinase 3
  • hsPEK
  • PEK
  • PEKPERKPancreatic eIF2-alpha kinase
  • PERK
  • PRKR-like endoplasmic reticulum kinase
  • WRS

Background

PERK, a type 1 ER membrane kinase, mediates eIF2 alpha phosphorylation at Ser51 during the UPR (unfolded protein response). Protein synthesis is inhibited, thereby reducing the burden of protein substrate for the ER folding and degradation mechanism. Phosphorylation of eIF2 alpha also selectively promotes the expression of UPR target genes such as Chop and BiP. PERK may also play a role in tumor cell adaptation to hypoxic stress by regulating the translation of angiogenic factors necessary for the development of functional microvessels. Mutations in PERK are responsible for the rare autosomal-recessive disorder, WRS (Wolcott-Rallison syndrome).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PERK Antibody (AF3999)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
All Applications
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Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF3999 Applications Species
Montes de Oca R, Alavi AS, Vitali N et al. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo Molecular Cancer Therapeutics 2021-10-01 [PMID: 34253590]
Himanshu Soni, Julia Bode, Chi D. L. Nguyen, Laura Puccio, Michelle Ne beta ling, Rosario M. Piro et al. PERK-mediated expression of peptidylglycine alpha -amidating monooxygenase supports angiogenesis in glioblastoma Oncogenesis 2/13/2020 [PMID: 32054826]
Q Xu, C Chen, G Chen, W Chen, D Zhou, Y Xie Significance of calreticulin as a prognostic factor in endometrial cancer Oncol Lett, 2018-04-13;15(6):8999-9008. 4/13/2018 [PMID: 29844817]
Chen Wang, Feng Zhang, Yu Cao, Mingming Zhang, Aixiu Wang, Mingcui Xu et al. Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress Scientific Reports 2016-09-29 [PMID: 27680712]
Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. PLoS ONE, 2015-03-17;10(3):e0119738. 2015-03-17 [PMID: 25780921] (Western Blot, Mouse) Western Blot Mouse
Atkins C, Liu Q, Minthorn E, Zhang S, Figueroa D, Moss K, Stanley T, Sanders B, Goetz A, Gaul N, Choudhry A, Alsaid H, Jucker B, Axten J, Kumar R Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity. Cancer Res, 2013-01-18;73(6):1993-2002. 2013-01-18 [PMID: 23333938] (Western Blot, Human) Western Blot Human
Liang G, Yang J, Wang Z Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha. Hum. Mol. Genet., 2008-07-29;17(20):3254-62. 2008-07-29 [PMID: 18664456] (Western Blot, Human) Western Blot Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PERK Products

Blogs on PERK.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK3
Uniprot