gp96/HSP90B1/GRP94 Antibody

Images

 
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis using Anti-HSP90B1 antibody NBP1-81803 (A) shows similar pattern to independent antibody NBP1-81802 (B).
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Orthogonal Strategies: Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining in human thyroid gland and skeletal muscle tissues using anti-HSP90B1 antibody. Corresponding HSP90B1 ...read more
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human cell line SK-BR-3.
Genetic Strategies: Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows high expression.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

gp96/HSP90B1/GRP94 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
Marker
ER Stress Marker
Specificity
Specificity of human gp96/HSP90B1/GRP94 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSP90B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:20
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
gp96/HSP90B1/GRP94 Lysate (NBP2-65662)
Control Peptide
gp96/HSP90B1/GRP94 Protein (NBP1-81803PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81803 in the following applications:

Publications
Read Publications using NBP1-81803.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (87%). Reactivity reported in scientific literature (PMID: 22363530)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody

  • 94 kDa glucose-regulated protein
  • ECGP
  • Endoplasmin
  • endothelial cell (HBMEC) glycoprotein
  • glucose regulated protein, 94 kDa
  • gp96
  • GP96ECGP
  • Grp94
  • GRP-94
  • GRP94gp96 homolog
  • Heat shock protein 90 kDa beta member 1
  • heat shock protein 90kDa beta (Grp94), member 1
  • HSP90B1
  • TRA1endoplasmin
  • tumor rejection antigen (gp96) 1
  • Tumor rejection antigen 1
  • Tumor rejection antigen-1 (gp96)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-81803
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for gp96/HSP90B1/GRP94 Antibody (NBP1-81803)(2)

Review for gp96/HSP90B1/GRP94 Antibody (NBP1-81803) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-81803:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot gp96/HSP90B1/GRP94 NBP1-81803
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reviewed by:
Takayuki Marutani
WB Human 09/06/2017
View

Summary

ApplicationWestern Blot
Sample TestedNeutrophilic cells whole cell lysate
SpeciesHuman
LotA114214

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gp96/HSP90B1/GRP94 Antibody (NBP1-81803) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for gp96/HSP90B1/GRP94 Antibody (NBP1-81803)

Discover related pathways, diseases and genes to gp96/HSP90B1/GRP94 Antibody (NBP1-81803). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for gp96/HSP90B1/GRP94 Antibody (NBP1-81803)

Discover more about diseases related to gp96/HSP90B1/GRP94 Antibody (NBP1-81803).
 

Pathways for gp96/HSP90B1/GRP94 Antibody (NBP1-81803)

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PTMs for gp96/HSP90B1/GRP94 Antibody (NBP1-81803)

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Recent Reviews

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1
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1
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Takayuki Marutani
09/06/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSP90B1