LSM4 Antibody (HL3172)

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LSM4 antibody [HL3172] detects LSM4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.LSM4 stained by LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LSM4 antibody [HL3172] detects LSM4 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LSM4 stained by LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

LSM4 antibody [HL3172] detects LSM4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.LSM4 stained by LSM4 antibody [HL3172] (NBP3-48776) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL3172
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

LSM4 Antibody (HL3172) Summary

• Description: Novus Biologicals Rabbit LSM4 Antibody (HL3172) (NBP3-48776) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human LSM4.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LSM4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for LSM4 Antibody (HL3172)

• Glycine-rich protein
• GRP
• LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• U6 snRNA-associated Sm-like protein LSm4
• YER112W

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LSM4