GnRH Antibody

Immunohistochemistry-Paraffin: GnRH Antibody [NB300-506] - Immunolocalization of GnRH in mouse embryo brain.

Immunohistochemistry-Paraffin: GnRH Antibody [NB300-506] - Analysis of Hyla cinerea (green treefrog) forebrain using GnRH Polyclonal Antibody. Tissues were blocked in 10% normal goat serum in 0.1M PBS and then stained. Staining was developed with DAB substrate for 10 minutes. Data courtesy of Innovators Program.

• Reactivity: Mu, Am
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GnRH Antibody Summary

• Description: Novus Biologicals Rabbit GnRH Antibody (NB300-506) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-GnRH Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.
• Specificity: GnRH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNRH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
• Application Notes: May be useful in IF and IHC-P. IF: Staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain. IHC: 4% paraformaldehyde is recommended as a fixative.
• Control:

  GnRHR Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literatures (PMID: 21303944 and 25958044).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GnRH Antibody

• GNRH
• GnRH-associated peptide 1
• gonadotropin-releasing hormone 1 (leutinizing-releasing hormone)
• gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
• GRH
• LHRH
• LNRH
• luliberin I
• Progonadoliberin I
• progonadoliberin-1
• prolactin release-inhibiting factor

Background

Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GnRH Antibody (NB300-506)(2)

Publications using NB300-506

• Li X, Xiao J, Fan Y et al. miR-29 family regulates the puberty onset mediated by a novel Gnrh1 transcription factor TBX21 Journal of Endocrinology 2019-09-01 [PMID: 31252405] (Mouse)
• Pandolfi EC, Hoffmann HM, Schoeller EL et al. Haploinsufficiency of SIX3 Abolishes Male Reproductive Behavior Through Disrupted Olfactory Development, and Impairs Female Fertility Through Disrupted GnRH Neuron Migration. Mol. Neurobiol. 2018-03-27 [PMID: 29589282] (Mouse)

Bioinformatics

• Gene Symbol: GNRH1
• Entrez:
  • 2796(Human)
  • 14715(Mouse)