The Use of RNA Polymerase II Antibodies In Proteasome Regulation Studies

Wed, 05/19/2010 - 07:49

At Novus Biologicals, we recently added a new RNA Polymerase II antibody (clone 4H8) to our antibody catalog. RNAPII is an essential transcription enzyme, catalyzing the transcription of DNA during the elongation stage of mRNA synthesis (known as the RNA polymerase II elongation complex.) RNAPII transcription is commonly recognized as the primary site of gene expression in eukaryotes, and is the most studied of the RNA polymerase enzymes.

Western Blot: RNA polymerase II Western Blot: RNA polymerase II

The initiation phase of RNAP II requires numerous transcription factors, to enable it to bind to promoter sites and thus begin RNA transcription. The enzyme co-ordinates RNA processing via Serine-2 and Serine-5 phosphorylation at the largest subunit C-terminal repeat. The new RNAPII antibody that we supply targets this region, in both the phosphorylated and unphosphorylated forms. It is effective in immunoblotting, immunprecipitation and ELISA antibody assays, and can also be used in ChIP tests.

In the past 15 years, numerous transcription factors have been discovered that play a pivotal role in RNAP II regulation, and which themselves have a diverse number of control mechanisms. Additionally, the catalytic mechanism of RNAP II has thrown new light on polymerase function.

Recently, RNAP II antibody products were used in ChIP studies into transcriptional inhibition of the luteinizing hormone beta (LHb) gene by proteasome blockade. LH release is controlled by Gonadotropin-releasing hormone (GnRH). GnRH disruption prevented recruitment of RNAP II to the LH-b promoter site, preventing release of LH. This proved that gene expression is highly dependent on transcription factor function, reinforcing the importance of RNAPII to our antibody database.

Novus Biologicals offers many RNA polymerase II reagents for your research needs including:

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