GnRHR Overexpression Lysate

Western Blot: Gonadotropin-releasing hormone receptor Overexpression Lysate (Adult Normal) [NBL1-11192] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Gonadotropin-releasing hormone receptor.

• Reactivity: Hu
• Applications: WB

GnRHR Overexpression Lysate Summary

• Description: Transient overexpression lysate of gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC214403 and based on accession number NM_000406. The protein contains a C-MYC/DDK Tag.
• Gene: GNRHR

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 37.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GnRHR Overexpression Lysate

• GnRH receptor
• GnRHR
• GnRH-R
• GNRHR1
• gonadotropin-releasing hormone (type 1) receptor 1
• gonadotropin-releasing hormone receptor
• GRHR
• HH7
• leutinizing-releasing hormone receptor
• LHRHR
• LHRHRleutinizing hormone releasing horomone receptor
• LRHR
• luliberin receptor
• Luliberin
• type I GnRH receptor

Background

This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GNRHR