GM-CSF Antibody - Azide and BSA Free

Western Blot: GM-CSF Antibody [NBP1-42743] - Analysis of a band 14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer.

Western blot using anti-Mouse GM-CSF antibody shows detection of a band ~14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight(TM)649 conjugated anti-Rabbit IgG

• Reactivity: Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GM-CSF Antibody - Azide and BSA Free Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  Purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment.
• Immunogen: This purified GM-CSF Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein. (Uniprot: P01587)
• Specificity: In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSF2
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:500-1:2000
• Application Notes: This purified antibody has been tested in western blot and is suitable for use in neutralizations and ELISA. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.3 kDa in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for GM-CSF Antibody - Azide and BSA Free

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor; CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF2
• Uniprot:
  • P01587()
  • P01587()
  • P01587()
  • P01587()