GM-CSF Antibodies

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_lightbox"> 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_thumb" />

215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND₅₀ is typically 0.03-0.09 µg/mL." class="big_lightbox"> 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND₅₀ is typically 0.03-0.09 µg/mL." alt="Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND₅₀ is typically 0.03-0.09 µg/mL." class="big_thumb" />

415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox"> 210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." alt="Human dendritic cells treated with LPS, Recombinant Human TNF-a (210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_thumb" />

215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-215-NA). The ND₅₀ is typically 0.08-0.16 µg/mL." class="big_lightbox"> 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-215-NA). The ND₅₀ is typically 0.08-0.16 µg/mL." alt="Recombinant Human GM-CSF (Catalog # 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-215-NA). The ND₅₀ is typically 0.08-0.16 µg/mL." class="big_thumb" />

415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA). The ND₅₀ is typically 0.01‑0.03 µg/mL." class="big_lightbox"> 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA). The ND₅₀ is typically 0.01‑0.03 µg/mL." alt="Recombinant Mouse GM-CSF (415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA). The ND₅₀ is typically 0.01‑0.03 µg/mL." class="big_thumb" />

415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1546-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine GM-CSF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1546). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> 1546-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine GM-CSF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1546). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Canine GM-CSF (Catalog # 1546-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine GM-CSF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1546). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1546-GM) was serially diluted and captured by Mouse Anti-Canine GM‑CSF Monoclonal Antibody (Catalog # MAB1546) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1546) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1546-GM) was serially diluted and captured by Mouse Anti-Canine GM‑CSF Monoclonal Antibody (Catalog # MAB1546) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1546) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM-CSF Monoclonal Antibody (Catalog # MAB5181). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM-CSF Monoclonal Antibody (Catalog # MAB5181). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Rat GM-CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM-CSF Monoclonal Antibody (Catalog # MAB5181). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="Recombinant Rat GM‑CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

518-GM) was serially diluted and captured by Mouse Anti-Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF518) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 518-GM) was serially diluted and captured by Mouse Anti-Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF518) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

711-PG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM-CSF (40 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine GM-CSF Monoclonal Antibody (Catalog # MAB711). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 711-PG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM-CSF (40 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine GM-CSF Monoclonal Antibody (Catalog # MAB711). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Porcine GM-CSF (Catalog # 711-PG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM-CSF (40 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine GM-CSF Monoclonal Antibody (Catalog # MAB711). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="GM-CSF was detected in immersion fixed differentiated human dendritic cells using Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB215R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215R). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_lightbox"> 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215R). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_thumb" />