GM-CSF Products

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">

215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_lightbox" target="_blank">

215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND₅₀ is typically 0.03-0.09 µg/mL." class="big_lightbox" target="_blank">

415-ML) was serially diluted and captured by Rat Anti-Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-415-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox" target="_blank">

215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">