Glypican 1 Antibody [Unconjugated]

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Glypican 1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519) at 10 µg/mL for 3 hours at room ...read more
MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4519, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Glypican 1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 1
Asp24-Ser530
Accession # P35052
Specificity
Detects human Glypican 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPC1
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using AF4519.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 1 Antibody [Unconjugated]

  • FLJ38078
  • Glypican 1
  • glypican proteoglycan 1
  • glypican
  • glypican-1
  • GPC1

Background

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 1 (GPC-1) is synthesized as a 558 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked and four potential O-linked sites for glycosylation or glycanation. There are potentially two heparan sulfate (HS) modifications on GPC-1 that could contribute to a native molecular weight of approximately 200 kDa (7, 8, 9). Mature human GPC-1 shares 91% aa identity with mature mouse GPC-1. There are two potential splice variants of human GPC-1. Both show an alternate start site at Met73, while one has an additional 65 aa substitution for the C-terminal 264 amino acids (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12‑15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). This is accomplished by having juxtramembrane HS attachment sites, and a flexible, GPI-linkage (17). Data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contract, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Lamoureux, F. et al. (2007) BioEssays 29:758.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. GenBank Accession # P35052.
  7. David, G. et al. (1990) J. Cell Biol. 111:3165.
  8. Lories, V. et al. (1992) J. Biol. Chem. 267:1116.
  9. Lories, V. et al. (1989) J. Biol. Chem. 264:7009.
  10. GenBank Accession # EAW71184.
  11. GenBank Accession # EAW71183.
  12. Chernousov, M.A. et al. (2006) J. Neurosci. 26:508.
  13. Wegrowski, Y. et al. (2006) Clin. Exp. Immunol. 144:485.
  14. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  15. Kayed, H. et al. (2006) Int. J. Oncol. 29:1139.
  16. Selleck, S.B. (2006) SciSTKE, April 4:pe17.
  17. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  18. Su, G. et al. (2006) Am. J. Pathol. 168:2014.
  19. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GPC1
Entrez
Uniprot