Glypican 3 Antibody (024) - Azide and BSA Free

Flow Cytometry: Glypican 3 Antibody (024) [NBP3-23451] - Flow cytometric analysis of Human Glypican 3 expression on HepG2 cells. Cells were stained with NBP3-23451, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

• Reactivity: Hu
• Applications: Flow
• Clone: 024
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glypican 3 Antibody (024) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Glypican 3 Antibody (024) - Azide and BSA Free

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPC3