Glutaminase Antibody (3211)

Images

 
Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [3211] ...read more
Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762] - Paraffin-embedded mouse liver. Glutaminase C antibody [3211] diluted at 1:250.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3211
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Glutaminase Antibody (3211) Summary

Immunogen
The exact sequence is proprietary.
Specificity
Specific for GAC but not KGA isoform.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GLS
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-43762 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Glutaminase Antibody (3211)

  • AAD20
  • EC 3.5.1.2
  • GLS
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • Glutaminase
  • glutaminase, phosphate-activated
  • K-Glutaminase
  • KIAA0838FLJ10358
  • L-Glutamine Amidohydrolase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutaminase Antibody (NBP2-43762)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(1)
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Filter By Species
Human
(1)
All Species
Showing Publication 1 - 1 of 1.
Publication using NBP2-43762 Applications Species
Chen Y, Zhao Y, Bajor D et al. A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues Journal of Genetics and Genomics Jan 1 2020 (ELISA, Human) ELISA Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glutaminase Antibody (NBP2-43762). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.
    • Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GLS