Glutaminase Antibody (3211) - Azide and BSA Free

Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody [3211] diluted at a dilution of 1:1000.

Glutaminase C antibody [GT3211] detects Glutaminase C protein at mitochondria by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutaminase C protein stained by Glutaminase C antibody [GT3211] (NBP2-43762) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.

Glutaminase C antibody [GT3211] detects Glutaminase C protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Glutaminase C antibody [GT3211] (NBP2-43762) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC
• Clone: 3211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutaminase Antibody (3211) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Glutaminase Antibody (3211) - Azide and BSA Free (NBP2-43762) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Glutaminase Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.
• Specificity: Specific for GAC but not KGA isoform.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLS
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Glutaminase Antibody (3211) - Azide and BSA Free

• AAD20
• EC 3.5.1.2
• GLS
• GLS1DKFZp686O15119
• glutaminase C
• glutaminase kidney isoform, mitochondrial
• Glutaminase
• glutaminase, phosphate-activated
• K-Glutaminase
• KIAA0838FLJ10358
• L-Glutamine Amidohydrolase

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutaminase Antibody (NBP2-43762)(3)

Publications using NBP2-43762

• Mincheva G, Gimenez-Garzo C, Izquierdo-Altarejos P et al. Golexanolone, a GABAA receptor modulating steroid antagonist, restores motor coordination and cognitive function in hyperammonemic rats by dual effects on peripheral inflammation and neuroinflammation CNS neuroscience & therapeutics 2022-07-26 [PMID: 35880480] (WB, Rat)
  
  Details:
  1:1000 WB dilution
• Chen Y, Zhao Y, Bajor D et al. A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues J Genet Genomics 2020-10-02 [PMID: 33004309]
• Wang, Y, Bucher, M Et al. Use of Glucose, Glutamine and Fatty Acids for Trophoblast Respiration in Lean, Obese and Gestational Diabetic Women. J Clin Endocrinol Metab 2019-05-22 [PMID: 31116396] (ICC/IF, Porcine, Bovine, Sheep, Mouse)

FAQs for Glutaminase Antibody (NBP2-43762). (Showing 1 - 1 of 1 FAQs).

1. I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.
   • Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.

Bioinformatics

• Gene Symbol: GLS