Western Blot: Glutaminase Antibody (3211) [NBP2-43762] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody  diluted at ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (3211) [NBP2-43762] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody  ...read more
Immunohistochemistry-Paraffin: Glutaminase Antibody (3211) [NBP2-43762] - Paraffin-embedded mouse liver. Glutaminase C antibody  diluted at 1:250.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP2-43762 in the following applications:
FAQs for Glutaminase Antibody (NBP2-43762). (Showing 1 - 1 of 1 FAQ).
I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.
Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Glutaminase Antibody (3211) and receive a gift card or discount.