Glut1 Antibody [FITC]

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Flow Cytometry: Glut1 Antibody [FITC] [NB110-39113F] - An intracellular stain was performed on HepG cells with Glut1 Antibody NB110-39113F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, Bv, PmSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

Glut1 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166]
Localization
Cell membrane, Cytoplasm (near membranes), Melanosome
Marker
Plasma Membrane Marker
Predicted Species
Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This GLUT1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 29456650). 100% sequence identity with primate, 93% sequence identity with bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Glut1 Antibody [FITC]

  • DYT17
  • DYT18
  • DYT18DYT17
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • GLUT1GLUT
  • HepG2 glucose transporter
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

GLUT1 is a major glucose transporter in the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are seven types of glucose transport carrier proteins, designated as Glut 1 to 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut1 Antibody (NB110-39113F) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Glut1 Antibody (NB110-39113F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-39113AF350
Alexa Fluor 405 NB110-39113AF405
Alexa Fluor 488 NB110-39113AF488
Alexa Fluor 532 NB110-39113AF532
Alexa Fluor 594 NB110-39113AF594
Alexa Fluor 647 NB110-39113AF647
Alexa Fluor 700 NB110-39113AF700
Alexa Fluor 750 NB110-39113AF750
Allophycocyanin/Cy7 NB110-39113APCCY7
Biotin NB110-39113B
DyLight 350 NB110-39113UV
DyLight 405 NB110-39113V
DyLight 488 NB110-39113G
DyLight 550 NB110-39113R
DyLight 594 NB110-39113DL594
DyLight 650 NB110-39113C
DyLight 680 NB110-39113FR
DyLight 755 NB110-39113IR
FITC NB110-39113F
HRP NB110-39113H
Janelia Fluor 549 NB110-39113JF549
Janelia Fluor 646 NB110-39113JF646
PE NB110-39113PE
PE/Atto594 NB110-39113PEATT594
PE/Cy5.5 NB110-39113PECY55
PE/Cy7 NB110-39113PECY7

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Bioinformatics Tool for Glut1 Antibody (NB110-39113F)

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Blogs on Glut1.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A1